Relative amount of innexin mRNA in the manipulated flies (elav-GAL4 > UAS-inx RNAi) comparing to the control flies (elav-GAL4/+ and UAS-inx RNAi/+) is shown. The results were normalized to the relative amount of ribosomal protein 49 (Rp49). The following primers were used (forward and reverse, respectively):
Inx1: 5'-GCATCAACATTATCGTGCAGAT-3' and 5'-ACACATAGACCATGGGATCCA-3'
Inx2: 5'-ATTGTATTGTGGACGAGATCCC-3' and 5'-TTGTGGTACTTCACCTCGTCC-3'
Inx3: 5'-GATCCTCAAGTATTTCGTGAACAGT-3' and 5'-ACATCCGTACCGTAGGACATG-3'
Inx4: 5'-TGCTGCCCCTGAATATACTGA-3' and 5'-GAAGCAACTGAAGACGCATTC-3'
Inx5: 5'-GATGTCCGGTTTGAACCTTCT-3' and 5'-GTTTGGTCATGAAACGCAACT-3'
Inx6: 5'-CTGCAGATGCTCACCAGGTAT-3' and 5'-CTGATGAACACATTGAGCAGCT-3'
Inx7: 5'-ATCAAGCGGCATGCATACTAC-3' and 5'-GTTAGACCCACCATTCGAAGG-3'
Inx8: 5'-TACAATTTGCACAACTTGCCC-3' and 5'-AGTTGTCGGTATTTTCTTTTGCC-3'
Rp49: 5'-CCAAGGACTTCATCCGCCACC-3' and 5'-GCGGGTGCGCTTGTTCGATCC-3'