An ancient yet flexible cis-regulatory architecture allows localized Hedgehog tuning by patched/Ptch1
- University of Michigan Medical School, United States
- Harvard University, United States
- National Institutes of Health, United States
- University of Southern California Keck School of Medicine, United States
- Keck School of Medicine of the University of Southern California, United States
Abstract
The Hedgehog signaling pathway is part of the ancient developmental-evolutionary animal toolkit. Frequently co-opted to pattern new structures, the pathway is conserved among eumetazoans yet flexible and pleiotropic in its effects. The Hedgehog receptor, Patched, is transcriptionally activated by Hedgehog, providing essential negative feedback in all tissues. Our locus-wide dissections of the cis-regulatory landscapes of fly patched and mouse Ptch1 reveal abundant, diverse enhancers with stage- and tissue-specific expression patterns. The seemingly simple, constitutive Hedgehog response of patched/Ptch1 is driven by a complex regulatory architecture, with batteries of context-specific enhancers engaged in promoter-specific interactions to tune signaling individually in each tissue, without disturbing patterning elsewhere. This structure-one of the oldest cis-regulatory features discovered in animal genomes-explains how patched/Ptch1 can drive dramatic adaptations in animal morphology while maintaining its essential core function. It may also suggest a general model for the evolutionary flexibility of conserved regulators and pathways.
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Reviewing Editor
- Janet Rossant, University of Toronto, Canada
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, and with the institutional animal care protocols of Harvard University and The Jackson Laboratories, where the animal experimentation was performed.Animal husbandry and all experiments were performed in accordance with the National Institute of Health guidelines and the Institutional Animal Care and Use Committee of the University of Southern California (protocol #11867).
Version history
- Received: December 4, 2015
- Accepted: May 3, 2016
- Accepted Manuscript published: May 5, 2016 (version 1)
- Version of Record published: May 31, 2016 (version 2)
Copyright
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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An ancient yet flexible cis-regulatory architecture allows localized Hedgehog tuning by patched/Ptch1
eLife 5:e13550.
https://doi.org/10.7554/eLife.13550
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