mRNA levels of genes inhibited by insulin in skeletal muscle (gastrocnemius), relative to those of untreated Gcgr+/- females (normalized to Actb, Gapdh, and Gusb) (N=4–6). Irs2, Insulin receptor substrate 2; Fbxo32, F-box only protein 32 (Atrogin-1); Trim63, Tripartite motif-containing 63 (MuRF1); 4e-bp1, Eukaryotic translation initiation factor 4E binding protein 1 (Eif4ebp1); Gadd45a, Growth arrest and DNA-damage-inducible 45 alpha; p21, Cyclin-dependent kinase inhibitor 1A (Cdkn1a). p27, Cyclin-dependent kinase inhibitor 1B (Cdkn1b). *p<0.05; **p<0.01; Mann-Whitney U test. Only groups that exhibited a > twofold regulation as compared to controls (dashed lines) were tested.