Direct neural pathways convey distinct visual information to Drosophila mushroom bodies

  1. Katrin Vogt
  2. Yoshinori Aso
  3. Toshihide Hige
  4. Stephan Knapek
  5. Toshiharu Ichinose
  6. Anja B Friedrich
  7. Glenn C Turner
  8. Gerald M Rubin  Is a corresponding author
  9. Hiromu Tanimoto  Is a corresponding author
  1. Max-Planck Institut für Neurobiologie, Germany
  2. Tohoku University Graduate School of Life Sciences, Japan
  3. Janelia Research Campus, Howard Hughes Medical Institute, United States
  4. Cold Spring Harbor Laboratory, United States
4 figures

Figures

Figure 1 with 1 supplement
The γd KCs are required for visual memory.

(A) γd neurons labeled by MB607B-GAL4. (B) 3D reconstruction of γd neurons labeled by MB607B-GAL4 (purple) in the entire MB (yellow). Arrow indicates atypical dendritic protrusion of the γd neurons. …

https://doi.org/10.7554/eLife.14009.002
Figure 1—figure supplement 1
Behavioral screen identifies the requirement of the γd KCs in aversive visual conditioning but not olfactory conditioning.

(A) Subsets of KC types labelled in GAL4 lines and the resulting learning defects in the behavioral screen using UAS-shits1. Expression levels (represented in the gray scale) are determined as …

https://doi.org/10.7554/eLife.14009.003
γd neurons are atypical KCs and respond to visual stimuli.

(A–B) Main output and input sites labeled by Syt::GFP (green) and DenMark::mCherry (red) are differentially localized to the dorsal γ lobe and the vAC (arrow). The MB lobe (A) and main calyx (B) are …

https://doi.org/10.7554/eLife.14009.004
Figure 3 with 3 supplements
VPNs directly convey optic lobe inputs to the MB vAC.

(A) VPN-MB1 neurons labeled by MB425B-GAL4. (B) A single VPN-MB1 neuron, generated by heat shock flip out, connects the medulla and the central brain. (C) VPN-MB2 neurons labeled by MB334C-GAL4. (D) …

https://doi.org/10.7554/eLife.14009.005
Figure 3—figure supplement 1
3D reconstruction of VPNs and γd neurons (purple: MB419B-GAL4) registered in a standard brain reveals overlapping processes in the vAC (arrow).

Yellow: entire MB. (A) Blue: MB425B-GAL4. (B) Green: MB334C-GAL4. Scale bar: 50 µm.

https://doi.org/10.7554/eLife.14009.006
Figure 3—figure supplement 2
VPN axons overlap with KC processes in the vAC labeled with DC0.

(A–C) The DC0 antibody highlights KCs including the γd neurons labeled by MB419B-GAL4. (D and E) The γd dendrites overlap with the processes of VPN-MB1 (MB425B-GAL4) (D) and VPN-MB2 (MB334C-GAL4) (E)…

https://doi.org/10.7554/eLife.14009.007
Figure 3—figure supplement 3
VPNs connect to the γd vAC.

(A–A”) Double labeling of VPN-MB1 (MB425B-GAL4, red) and KCs (R13F02-LexA for labeling most KCs, green) reveals overlap in the vAC (arrows). (B–B”) Double labeling of VPN-MB2 (MB334C-GAL4, red) and …

https://doi.org/10.7554/eLife.14009.008
Figure 4 with 2 supplements
VPN-MB1 and VPN-MB2 convey distinct visual features.

(A) OPNs labeled by GH146-GAL4 are not required for visual color conditioning (one-way ANOVA, p>0.05), n = 8. (B–C) VPN-MB1 (MB425B-GAL4; B), but not VPN-MB2 (MB334C-GAL4; C), are required for color …

https://doi.org/10.7554/eLife.14009.009
Figure 4—figure supplement 1
The blockade of similar VPNs without vAC connection does not impair color discrimination learning.

(A and B) MB425B-GAL4 (A) and VT008475-GAL4 label VPN-MB1 and similar VPNs that do not extend to the vAC (arrows). Scale-bars represents 50 µm. (C) Blocking output of neurons labeled by VT008475-GAL4

https://doi.org/10.7554/eLife.14009.010
Figure 4—figure supplement 2
The blockade of MBON-a1 does not impair intensity discrimination learning.

(A) MB331C-GAL4 labels MBON-a1 (arrows) in addition to VPN-MB2. (B) MB310C-GAL4 labels MBON-a1 (arrows) but not VPN-MB2. Scale-bars represents 50 µm. (C) Blocking output of neurons labeled by MB310C-…

https://doi.org/10.7554/eLife.14009.011

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