Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
- Rutgers, The State University of New Jersey, United States
Figures
Figure 1 with 2 supplements
ChR2/tdTomato expression in whisker pad muscles and optical activation of whisker movements in Emx1-Cre;Ai27D mice.
(A) Illustration of experimental setup showing the position of 460 nm light spot on the whisker pad used for optogenetic stimulation. (B) Example color map from one mouse (isoflurane anesthesia, …
Figure 1—figure supplement 1
Analysis of retraction and protraction movements for individual whiskers.
(A) Schematic of experiment as in Figure 1, with video image of tracked whiskers. (B) Color maps showing the peak whisker movement evoked by a 20 ms, 460 nm light spot located at various positions …
Figure 1—figure supplement 2
Absence of ChR2/tdTomato expression in vibrissal nerve fibers of EMX-cre;Ai27D mice.
(A) Example images of two whisker follicles in an Emx1-Cre;Ai27D mouse. Expression of ChR2/tdTomato was present in intrinsic muscle (m.) surrounding the follicle, but was not observed in nerve …
Figure 2
Characterization of whisker movements evoked by optogenetic whisker pad stimulation.
(A) Top: Illustration of experiment setup (isoflurane anesthesia, 0.8–1.5%). Rostral whisker pad illumination (460 nm) was used to evoke whisker protractions (positive angle values). Bottom: Image …
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Figure 2—source data 1
Data for Figure 2B.
- https://doi.org/10.7554/eLife.14140.007
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Figure 2—source data 2
Data for Figure 2C.
- https://doi.org/10.7554/eLife.14140.008
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Figure 2—source data 3
Data for Figure 2D.
- https://doi.org/10.7554/eLife.14140.009
Figure 3
Extracellular recordings of S1 activity in response to optogenetic whisker pad stimulation.
(A) Illustration of experiment setup (isoflurane anesthesia, 0.8–1.5%), including chronically implanted microwire array. (B) Example peri-stimulus time histograms (PSTHs; bin size, 10 ms) for one …
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Figure 3—source data 1
Data for Figure 3D.
- https://doi.org/10.7554/eLife.14140.011
Figure 4
Behavioral performance in mice trained to detect optogenetic stimulation of the whisker pad.
(A) Illustration of the behavioral task. Water deprived, head-fixed mice were rewarded with water for licking within a 2 s response window (gray boxes) after optical stimulation (460 nm) of the …
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Figure 4—source data 1
Data for Figure 4B.
- https://doi.org/10.7554/eLife.14140.013
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Figure 4—source data 2
Data for Figure 4C.
- https://doi.org/10.7554/eLife.14140.014
Videos
Video 1
Whisker movements in response to a light pulse at whisker pad position (0, 0), as in Figure 1—figure supplement 1.
The blue square at the upper right indicates the timing of optogenetic stimulation (20 ms, 460 nm). The original sampling rate of 500 frames per second was slowed to 25 frames per second for display.
Video 2
Whisker movements in response to a light pulse at whisker pad position (-3, -2), as in Figure 1—figure supplement 1.
The blue square at the upper right indicates the timing of optogenetic stimulation (20 ms, 460 nm). The original sampling rate of 500 frames per second was slowed to 25 frames per second for display.
Video 3
Whisker movements in response to a light pulse at whisker pad position (-2, -2), as in Figure 1—figure supplement 1.
The blue square at the upper right indicates the timing of optogenetic stimulation (20 ms, 460 nm). The original sampling rate of 500 frames per second was slowed to 25 frames per second for display.
