Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice

  1. Sunmee Park
  2. Akhil Bandi
  3. Christian R Lee
  4. David J Margolis  Is a corresponding author
  1. Rutgers, The State University of New Jersey, United States
4 figures and 3 videos

Figures

Figure 1 with 2 supplements
ChR2/tdTomato expression in whisker pad muscles and optical activation of whisker movements in Emx1-Cre;Ai27D mice.

(A) Illustration of experimental setup showing the position of 460 nm light spot on the whisker pad used for optogenetic stimulation. (B) Example color map from one mouse (isoflurane anesthesia, …

https://doi.org/10.7554/eLife.14140.003
Figure 1—figure supplement 1
Analysis of retraction and protraction movements for individual whiskers.

(A) Schematic of experiment as in Figure 1, with video image of tracked whiskers. (B) Color maps showing the peak whisker movement evoked by a 20 ms, 460 nm light spot located at various positions …

https://doi.org/10.7554/eLife.14140.004
Figure 1—figure supplement 2
Absence of ChR2/tdTomato expression in vibrissal nerve fibers of EMX-cre;Ai27D mice.

(A) Example images of two whisker follicles in an Emx1-Cre;Ai27D mouse. Expression of ChR2/tdTomato was present in intrinsic muscle (m.) surrounding the follicle, but was not observed in nerve …

https://doi.org/10.7554/eLife.14140.005
Characterization of whisker movements evoked by optogenetic whisker pad stimulation.

(A) Top: Illustration of experiment setup (isoflurane anesthesia, 0.8–1.5%). Rostral whisker pad illumination (460 nm) was used to evoke whisker protractions (positive angle values). Bottom: Image …

https://doi.org/10.7554/eLife.14140.006
Extracellular recordings of S1 activity in response to optogenetic whisker pad stimulation.

(A) Illustration of experiment setup (isoflurane anesthesia, 0.8–1.5%), including chronically implanted microwire array. (B) Example peri-stimulus time histograms (PSTHs; bin size, 10 ms) for one …

https://doi.org/10.7554/eLife.14140.010
Behavioral performance in mice trained to detect optogenetic stimulation of the whisker pad.

(A) Illustration of the behavioral task. Water deprived, head-fixed mice were rewarded with water for licking within a 2 s response window (gray boxes) after optical stimulation (460 nm) of the …

https://doi.org/10.7554/eLife.14140.012

Videos

Video 1
Whisker movements in response to a light pulse at whisker pad position (0, 0), as in Figure 1—figure supplement 1.

The blue square at the upper right indicates the timing of optogenetic stimulation (20 ms, 460 nm). The original sampling rate of 500 frames per second was slowed to 25 frames per second for display.

https://doi.org/10.7554/eLife.14140.015
Video 2
Whisker movements in response to a light pulse at whisker pad position (-3, -2), as in Figure 1—figure supplement 1.

The blue square at the upper right indicates the timing of optogenetic stimulation (20 ms, 460 nm). The original sampling rate of 500 frames per second was slowed to 25 frames per second for display.

https://doi.org/10.7554/eLife.14140.016
Video 3
Whisker movements in response to a light pulse at whisker pad position (-2, -2), as in Figure 1—figure supplement 1.

The blue square at the upper right indicates the timing of optogenetic stimulation (20 ms, 460 nm). The original sampling rate of 500 frames per second was slowed to 25 frames per second for display.

https://doi.org/10.7554/eLife.14140.017

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