(A) Schematic of multi-area two-photon microscope. Light from a Ti:sapphire laser is split into two beams and one beam sent to a delay line. Each beam then enters a focal plane unit (FPU), which …
The point-spread function (PSF) was measured using 200 nm beads (Fluoresbrite Plain YG Microspheres, Polysciences Inc) at 840 nm excitation. Off-axis positions were accessed by translating a focal …
Neurons expressing YC-Nano140 were imaged with a single beam exciting fluorescence either in sub-area 1 or 2 (Average of n = 49 frames with motion correction). The detected signal in the …
(A) Viral injection scheme for simultaneous labeling of feedforward and feedback neurons and YC-Nano140 expression. (B) Functional mapping of S1 and S2 through optical intrinsic signal imaging. …
Optimized low tensor rank across animals.
Table of optimum column size of each factor matrices related to neurons (N’ + N’offset), time points (T’), and trial conditions (C’) determined after cross-validation and cost function procedures for each animal used for denoising. Total possible column sizes are also indicated along with number of active neurons.
(A) Calcium responses from one animal across multiple sessions are organized into a data tensor. The tensor is decomposed and a low-rank tensor representing denoised calcium responses is generated. …
(A) General linear model (GLM) of behavior-related responses. Example of GLM fit for one neuron of calcium responses against touch, licking, and whisking as behavior events. Single-trial calcium …
(A–B) Example of GLM fit for neurons showing prominent whisking [A] and licking [B] related calcium responses against touch, licking, and whisking behavior events. Single-trial calcium responses are …
(A) While LDA is performed on multiple simultaneously imaged neurons, for demonstration purposes, here calcium transients of two simultaneously imaged neurons within an imaging plane are plotted and …
(A) Analysis of coordinated activity across S1 and S2. Left panel shows example of single-trial population responses for Hit trials projected along Hit vs. CR axis for simultaneously imaged S1 (LDS1)…
Average S1 or S2 population responses across the first second prior to and following whisker touch onset for: (A) FA vs. CR trials; (C) high- vs. low-amplitude whisking CR trials; (E) target vs. …
(A) LDCCS1:S2 for target vs. non-target textures under non-task condition trials. (B) LDCCS1:S2 for P280 vs. P1200 textures for CR trials. We observed no increased or different LDCCS1:S2 between …
(A) The contribution of specific cell types to coordinated activity across S1 and S2 is measured by trial-shuffling responses for those cell types prior to calculating the LDCCS1:S2. The resulting …
(A), An example of population response after trial shuffling. Trial responses of neuronal subpopulations were shuffled in order to determine their contribution to the population response. For the …
(A) Cumulative distribution of average peak calcium responses for each cell type. (B) Average S1 (top) or S2 (bottom) population responses projected along the Hit vs. CR axis across the first second …
(A) Contribution of S1S2 and S2S1 neurons or S1ND and S2ND neurons to coordinated S1 and S2 activity for Hit trials along the Hit vs. CR axis after aligning to whisker-touch onset (dotted line). (B) …
Our results identify coordinated activity patterns across S1 and S2 that are related to motor behaviors, which could arise from common input from M1 or POm or indirect cortico-thalamocortical (CTC) …
Single area images from the multi-area two-photon microscope of L2/3 neurons were taken from 70-210 µm below the pial surface at 1 µm z-step resolution. Sub-area excitation beam was delivered …
Single trial video of calcium responses during texture discrimination acquired at 7 Hz with the multi-area two-photon microscope (1x playback speed). YFP (green) and CFP (blue) fluorescence from …
Axes used for linear discriminant analysis. Summary of trial conditions compared and used for linear discriminant analysis. For each axes, noted are potential differences in texture, licking, and …
Axes for LDA | Texture | Licking | Whisking | Utility in analysis |
---|---|---|---|---|
Hit vs. CR | Different | Different (Hit) | Same | Cannot isolate sensory, decision, or action-related responses |
FA vs. CR | Same | Different (FA) | Same | Isolate decision and action-related responses |
Pre- vs. post-touch licking (FA trials) | Same | Different | Same | Isolate licking-related responses |
High vs. Low Whisking (CR trials) | Same | None | Different | Isolate whisking-related responses |
P280 vs. P1200 (CR trials) | Different | None | Same | Isolate sensory-related responses |
Target vs. Non-target (Non-task) | Different | None | None | Isolate sensory-related responses |