Adenosine diphosphate restricts the protein remodeling activity of Hsp104 chaperone to Hsp70 assisted disaggregation

  1. Agnieszka Kłosowska
  2. Tomasz Chamera
  3. Krzysztof Liberek  Is a corresponding author
  1. University of Gdansk, Poland

Abstract

Hsp104 disaggregase provides thermotolerance in yeast by recovering proteins from aggregates in cooperation with the Hsp70 chaperone. Protein disaggregation involves polypeptide extraction from aggregates and its translocation through the central channel of Hsp104 hexamer. This process relies on adenosine triphosphate (ATP) hydrolysis. Considering that Hsp104 is characterized by low affinity towards ATP and is strongly inhibited by adenosine diphosphate (ADP), we asked how Hsp104 functions at the physiological levels of adenine nucleotides. We demonstrate that physiological levels of ADP highly limit Hsp104 activity. This inhibition, however, is moderated by the Hsp70 chaperone, which allows for efficient disaggregation by supporting Hsp104 binding to aggregates but not to non-aggregated, disordered protein substrates. Our results point to an additional level of Hsp104 regulation by Hsp70, which restricts the potentially toxic protein unfolding activity of Hsp104 to the disaggregation process, providing the yeast protein-recovery system with substrate specificity and efficiency in ATP consumption.

Article and author information

Author details

  1. Agnieszka Kłosowska

    Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdańsk, Poland
    Competing interests
    The authors declare that no competing interests exist.
  2. Tomasz Chamera

    Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdańsk, Poland
    Competing interests
    The authors declare that no competing interests exist.
  3. Krzysztof Liberek

    Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdańsk, Poland
    For correspondence
    liberek@biotech.ug.edu.pl
    Competing interests
    The authors declare that no competing interests exist.

Copyright

© 2016, Kłosowska et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,810
    views
  • 418
    downloads
  • 20
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Agnieszka Kłosowska
  2. Tomasz Chamera
  3. Krzysztof Liberek
(2016)
Adenosine diphosphate restricts the protein remodeling activity of Hsp104 chaperone to Hsp70 assisted disaggregation
eLife 5:e15159.
https://doi.org/10.7554/eLife.15159

Share this article

https://doi.org/10.7554/eLife.15159