Mitochondrial support of persistent presynaptic vesicle mobilization with age-dependent synaptic growth after LTP
- University of Texas at Austin, United States
- University of Colorado Denver, United States
- New York University, United States
- QPS, LLC, United States
- The University of Texas at Austin, United States
Abstract
Mitochondria support synaptic transmission through production of ATP, sequestration of calcium, synthesis of glutamate, and other vital functions. Surprisingly, less than 50% of hippocampal CA1 presynaptic boutons contain mitochondria, raising the question of whether synapses without mitochondria can sustain changes in efficacy. To address this question, we analyzed synapses from postnatal day 15 (P15) and adult rat hippocampus that had undergone theta-burst stimulation to produce long-term potentiation (TBS-LTP) and compared them to control or no stimulation. At 30 and 120 minutes after TBS-LTP, vesicles were decreased only in presynaptic boutons that contained mitochondria at P15, and vesicle decrement was greatest in adult boutons containing mitochondria. Presynaptic mitochondrial cristae were widened, suggesting a sustained energy demand. Thus, mitochondrial proximity reflected enhanced vesicle mobilization well after potentiation reached asymptote, in parallel with the apparently silent addition of new dendritic spines at P15 or the silent enlargement of synapses in adults.
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Funding
National Institutes of Health (NS201184)
- Kristen M Harris
Texas Emerging Technology Fund
- Kristen M Harris
National Institutes of Health (MH095980)
- Kristen M Harris
National Institutes of Health (NS074644)
- Kristen M Harris
National Institutes of Health (MH096459)
- Deborah J Watson
Brain Research Foundation
- Kristen M Harris
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2016, Smith et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Mitochondrial support of persistent presynaptic vesicle mobilization with age-dependent synaptic growth after LTP
eLife 5:e15275.
https://doi.org/10.7554/eLife.15275
