(a–c) Immunolabeled repo>GFP wing (glia in green: anti-GFP; neurons in red: anti-22c10) at different stages. (a) Initiation of migration, (b) reaching the level of the costa, and (c) migration …
(a–f) 18 hAPF repo>GFP/+ wing, immunolabeled with anti-22c10 (neurons in red), anti-Fra (gray) and anti-GFP (glia in green) shows the presence of Fra in the glial soma (white arrowheads) at the …
(a, b) 28 hAPF wings labeled with anti-Repo (glial nuclei): (a) WT wing showing complete migration;(b) fra3/+ wing showing incomplete migration (dashed box). (c) Histogram representing the migratory …
Migratory index and repo count of of fra3 wings in WT background.
This file contains underlying raw data for Figure 2.
(a–b) Histogram representing the MI upon fra knock down (fra KD) or overexpression (fra GOF) using the gcm>GFP/+ line. In (b), the MI was calculated at 24 hAPF. The MI was calculated using the …
Migratory index and time-lapse analysis of fra conditional mutants in the gcm>GFP/+ background.
This file contains underlying raw data for Figure 3.
(a) Histogram representing the MI of the indicated genotypes, calculated using the membrane GFP transgenic line (UAS-mCD8-GFP). (b) Histogram showing the percentage of wings with axonal delay in gcm>…
Summarizing the role of neurons in glia migration and the genetic interaction between gcm and fra in different transheterozygote combinations.
This file contains underlying source data for Figure 3—figure supplement 1 and 2.
(a) Graph showing the speed of the most-proximal glial cell of the chain (μm/h, y-axis; hAPF, x-axis) in gcm>GFP/+ and in gcm>fra GOF glia. The distance covered by the front cell is measured by …
(a) MI in fra mutants, gcm LOF and rescues of the indicated genotypes: gcm>GFP/+, gcm/fra LOF, gcm>gcm LOF, gcm>gcm KD and gcm>gcm GOF wings. (b) MI calculated at 24 hAPF in gcm>gcm GOF wings. The …
Summary of the role of gcm in glia migration.
This file contains underlying raw data for Figure 4.
(a) Bar chart showing the numbers of L1 glial nuclei in the various genotypes. (b) MI in the various genotypes, calculated using the membrane GFP transgenic line.
Effects of gcm on glia migration are independent of any change in the number of repo+ cells.
This file contains underlying raw data for Figure 5.
(a–a”) gcm>GFP/+ and (b–b”) gcm>GFP/+;UAS-gcm-RNAi/+ 17 hAPF wings immunolabeled with anti-Elav (neuronal nuclei in blue) and anti-Repo (glial nuclei in red). Note the glial cells converted into …
(a) Schematic representation of the fra gene indicated in blue. Lines indicate the introns, thin regions indicate the untranslated sequences, thick regions indicate the coding exons, pale blue …
(a) Western blot analysis on total protein extracts of transfected S2 cells showing the GFP levels from the fra-GFP reporter plasmid (representative Western Blot shows one out of a sample of three). …
(a) MI of the indicated genotypes. Histogram shows the MIs quantified for WT, for NetAΔ or NetBΔ single mutant wings and for NetAΔNetBTM or NetAΔNetBmyc wings. (b) NP4151-Gal4 driven GFP expression …
Summary of the role of Netrins in glia migration.
This file contains underlying raw data for Figure 7.
(a–c) Graphs representing the MI of the indicated genotypes, calculated by nuclear labeling.
(a) Schematic of axonal bundles in a 10 hAPF wing, the green rectangle indicates the region shown in (b). (b) NP4151-Gal4-driven GFP expression of NetB in a 10 hAPF wing. Proximal NetB expression as …
(a–d”’) Wing immunolabeled with anti-22c10 (red), anti-Unc5 (gray) and anti-GFP (green) in the transgenic line gcm>GFP/+ at different migratory stages. Unc5 starts being expressed in glia and …
Summary of role of unc5 in glia migration.
This file contains underlying raw data for Figure 8.
(a–a”) gcm>GFP/+ wing immunolabeled at 15 hAPF shows that the Unc5 protein cannot be detected at this stage. Note that (a–a”) are comprised of a few sections rather than maximum confocal …
(a) Histogram showing the endogenous expression of fra upon S2 cell transfection with a Repo expression vector. The y-axis represents the relative expression levels in cells transfected with Repo …
Summary of role of repo in glia migration.
This file contains underlying raw data for Figure 9.
(a) Graphical representation of the migratory behavior of gcm>GFP/+ and gcm>unc5 GOF wings at the Initiation phase (n=9). (b) Box plot showing the raw data, the mean and the standard errors for the …
Note the presence of the mCD8GFP in the axons, in the glia (Repo-positive cells in red, arrows) and in the epithelium (asterisk).