(a) Reconstructed morphologies and distribution of apical dendritic gemmules across the MCL (black bars), EPL (grey bars), and GL (red bars) of 3 representative sGCs. Grey/red ticks represents the midpoint of the EPL/mean of the cell’s gemmule distribution. (b,c) Spiking response (b) and synaptic input (c) of the 3 sGCs shown in a following activation of a single glomerulus superficial to the targeted GC. (d−f) Same as a-c for 3 representative dGCs. (g) Distribution of apical dendritic gemmules across reconstructed sGCs and dGCs. (h) A greater proportion of sGCs than dGCs fired in response to glomerular activation (Chi-square test, p = 4.2 × 10–3). (i) Excitatory input to sGCs exhibited larger peak currents (rank-sum test, p = 8.5 × 10–3) and charge transferred (rank-sum test, p = 0.046) than excitatory input to dGCs. No difference in excitation latency was observed (6.6 ± 11.6 vs. 9.7 ± 11.4 ms; rank-sum test, p = 0.12). Scalebar: 0.2 s/10 pA (inset: 40 ms/20 pA). (j,k) sGCs and dGCs showed significantly different firing rate-current (FI) curves in response to somatic step current injection (2-way ANOVA, p = 4.1 × 10–3). Individual (j) and mean (k) FI curves shown. Dashed lines show diminished firing due to depolarization block. (l) sGC action potentials exhibited more hyperpolarized thresholds (unpaired t test, p = 4.8 × 10–3), larger amplitudes (unpaired t test, p = 1.1 × 10–4), and faster rising slopes (unpaired t test, p = 2.9 × 10–4) than dGC action potentials. Inset: action potential phase plot. Scalebar: 30mV/100 mVms−1; dashed lines show origin. Shaded regions show mean ± SEM.