(A–J) Adult wings. (A,F) Control, phenotypically wild type nub-gal4 wing. The posterior compartment is marked in grey, and the bar marks the distance between anterior and posterior crossveins. (B,G). dlish04/Df(2R)Exel7150 wing with reduced crossvein spacing (B) and proximal hair PCP defects (G). (C,H) nub-gal4 UAS-dlish-RNAi (VDRC) wing with reduced area (significant by Student’s T and Whitney-Mann tests; see Figure 7—source data 1 for data analysis) and PCP defects (H). (D) Posterior-specific, hh-gal4-driven UAS-dlish-RNAi (VDRC) reduces posterior size compared to grey control area from A. (E) dlish4506 homozygous wing showing reduced crossvein spacing; hair PCP defects are similar to dlish04 (detail not shown). (I,J) Posterior, hh-gal4-driven expression of UAS-dlish-FLAG (I) or UAS-dlish (J) increases posterior size compared to grey control area from A. (K1,K2) Reduction of subapical anti-Dlish staining in dlish04 clone marked by absence of GFP (red). Because the GFP is in nuclei, the clone marker image is from the more basal, nuclear focal plane and slightly out of register with subapical Dlish. (L–N2) Posterior, hh-gal4 driven expression of UAS-dlish-RNAi decreases cortical, subapical Dachs (L) and increases basolateral, cytoplasmic Dachs (M); N1 and N2 show the Dachs changes in the same cross-section Z series projection that shows Dlish in Figure 6B2, with Engrailed (En) marking the posterior (p) versus anterior (a). (O1,O2) dlish04 homozygous clone, marked by absence of GFP, increases basolateral, cytoplasmic Dachs. (P1–Q2) Posterior, hh-gal4-driven expression of UAS-dlish-FLAG increases expression of the Yorkie activity reporter ex-lacZ (P1,P2) and increases subapical Dachs (Q1,Q2); anterior marked with Ci (P1) or posterior with GFP (Q1).