(a) Low magnification images of sagittal sections through the entire cerebellum from postnatal day (P) 2 through P21 showing the abundant immunolabeling for p75NTR in the EGL, which decreases by …
Sections from WT or Ngfr-/- mouse P7 cerebellum were processed for TUNEL labeling. Few labeled cells were detected and no difference between genotypes was observed.
(a) Confocal image of p75NTR and the proliferation marker Ki67 showing colocalization in the external EGL. Note that p75NTR is also expressed in developing Purkinje cells. (b) p75NTR is …
(a) Low magnification images of the entire cerebellum showing incorporation of EdU at postnatal ages from P5 to P14. GCPs in WT mice began to decrease EdU incorporation at P7, which continued …
Mean number of labeled cells of 3 slides for each animal, and statistical analysis for the graphs shown in 3c, 3e, and 3g.
GCPs from P7 rat were cultured with BrdU for 48 hr in the absence or presence of Shh with or without the different mature neurotrophins. BrdU labeling was analyzed by in-cell Western on the LiCor …
Mean values for each experiment and statistical analysis for all graphs.
(a) GCPs were cultured from P7 rat cerebella with BrdU in the absence or presence of Shh with or without 4 or 8 ng/ml of proNGF, proBDNF or proNT3 for 48 hr. Cells were analyzed by in-cell Western …
Mean values for each experiment and statistical analysis for all graphs in Figure 5.
Cerebellar neurons were cultured from P7 rat and treated overnight with proNT3. Cells were labeled with TUNEL and Dapi, and expressed as the ratio of TUNEL-positive cells to total cell number.
Hippocampal neurons were cultured from E18 rat embryos and treated overnight with 4 ng/ml of the indicated proneurotrophin, eliciting loss of approximately 30% of the cells, consistent with previous …
(a) GCPs from wild type or Ngfr-/- mice were cultured with Shh, proNT3 or Shh+proNT3 for 48 hr in the presence of BrdU and analyzed by in-cell Western analysis of anti-BrdU. (b) Immunostaining for …
Mean values for each experiment and statistical analysis for graphs in 6A and 6c.
(a) Immunostaining of P7 and P14 rat cerebellum with an antibody to the pro domain of proNT3 shows the presence of proNT3 in Purkinje cells, labeled with calbindin. Note the abundant proNT3 labeling …
Total brain lysates from Ntf3+/+, Ntf3+/-, and Ntf3-/- mice were immunoprecipitated with goat anti-proNT3 or goat control IgG, and probed with anti-proNT3 on a Western blot. ProNT3 produced from HEK …
(a) Immunostaining for HDAC1 in WT and Ngfr-/- mice at P14. Size bar is 20 µm. (b) High magnification images of HDAC1 staining in the EGL in WT and Ngfr-/- mice at P14. Size bar is 10 µm. (c) …
Mean values for each experiment and statistical analysis for all graphs in Figure 8.
(a) Ngfrfl/fl mice crossed with the Atoh1-Cre show lack of p75NTR specifically in the EGL while retaining p75NTR expression in Purkinje cells and meninges. Size bar indicates 20 µm. (b) Both Ngfr-/- …
Mean number of labeled cells of 3 slides for each animal, and statistical analysis for all graphs in Figure 9.
(a) Cerebellar size of both Ngfr-/- and Ngfrfl/fl-Atoh1-Cre was increased in the adult compared to WT mice. * indicates significance at p=0.0003. (b) Motor balance on the rotarod was impaired in …
Mean values for each experiment and statistical analysis for all graphs in Figure 10.