Pro-death NMDA receptor signaling is promoted by the GluN2B C-terminus independently of Dapk1
- University of Edinburgh, United Kingdom
- Trinity College Dublin, Ireland
- University of Melbourne, Australia
- Wellcome Trust Sanger Institute, United Kingdom
Figures
Figure 1 with 1 supplement
Neither Dapk1 nor excitotoxic insults increase GluN2B phosphorylation on Ser-1303.
(A,B) Strong excitotoxic insults induce GluN2B Ser-1303 dephosphorylation at later timepoints. Western analysis of extracts from cortical neurons treated as indicated with NMDA or bicuculline (50 …
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Figure 1—source data 1
Data relating to Figure 1—figure supplement 1a.
- https://doi.org/10.7554/eLife.17161.003
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- https://doi.org/10.7554/eLife.17161.004
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- https://doi.org/10.7554/eLife.17161.005
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- https://doi.org/10.7554/eLife.17161.006
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- https://doi.org/10.7554/eLife.17161.007
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Data relating to Figure 1f.
- https://doi.org/10.7554/eLife.17161.008
Figure 1—figure supplement 1
(A,B) Neurons were treated with staurosporine (STS, 1 µM) or FK-506 (FK, 5 µM)+okadaic acid (OA, 10 µM) for one hour, after which protein was harvested and western analysis for Phospho- (GluN2B Ser-1303) levels performed.
p=0.0023 (1-way ANOVA). Individual P-values left-to-right: 0.026, 0.047 (n = 8 (FK +OA); n = 4 (STS). (C) Schematic depicting the amino-acid changes resulting from mutations of the Grin2b gene in …
Figure 2
Excitotoxic and ischemic insults are not ameliorated by Dapk1 deficiency.
(A, B) NMDA-induced neuronal death is independent of Dapk1. Cortical neurons at DIV10 (A) or DIV16 (B) were treated as indicated for 1 hr, with neuronal death assessed at 24 hr. The p values relate …
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Figure 2—source data 1
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- https://doi.org/10.7554/eLife.17161.011
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- https://doi.org/10.7554/eLife.17161.012
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- https://doi.org/10.7554/eLife.17161.013
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- https://doi.org/10.7554/eLife.17161.014
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Data relating to Figure 2e–g.
- https://doi.org/10.7554/eLife.17161.015
Figure 3 with 1 supplement
Both TAT-NR2BCT and TAT-sNR2BsCT are direct NMDAR antagonists.
(A–D) Both TAT-NR2BCT and TAT-sNR2BCT (scrambled version of TAT-NR2BCT) immediately antagonize NMDAR currents upon extracellular exposure. NMDA-induced currents were recorded under whole-cell …
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Figure 3—source data 1
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- https://doi.org/10.7554/eLife.17161.017
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- https://doi.org/10.7554/eLife.17161.018
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- https://doi.org/10.7554/eLife.17161.019
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- https://doi.org/10.7554/eLife.17161.020
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Figure 3—source data 5
Data relating to Figure 3f.
- https://doi.org/10.7554/eLife.17161.021
Figure 3—figure supplement 1
(A) Neurons were treated where indicated with 50 µM TAT-NR2BCT for 1 hr, with death assessed after 24 hr.
(B) Neurons were pre-treated where indicated with TAT-NR2BCT or TAT-sNR2BCT for 1 hr, prior to 1 hr NMDA treatment at the indicated concentrations, in the continued presence of the peptides where …
