1. Neuroscience

Drosophila larval to pupal switch under nutrient stress requires IP3R/Ca2+ signalling in glutamatergic interneurons

  1. Siddharth Jayakumar
  2. Shlesha Richhariya
  3. O Venkateswara Reddy
  4. Michael J Texada
  5. Gaiti Hasan  Is a corresponding author
  1. Tata Institute of Fundamental Research, India
  2. Janelia Research Campus, Howard Hughes Medical Institute, United States
https://doi.org/10.7554/eLife.17495
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  1. Siddharth Jayakumar
  2. Shlesha Richhariya
  3. O Venkateswara Reddy
  4. Michael J Texada
  5. Gaiti Hasan
(2016)
Drosophila larval to pupal switch under nutrient stress requires IP3R/Ca2+ signalling in glutamatergic interneurons
eLife 5:e17495.
https://doi.org/10.7554/eLife.17495
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Abstract

Neuronal circuits are known to integrate nutritional information, but the identity of the circuit components is not completely understood. Amino acids are a class of nutrients that are vital for the growth and function of an organism. Here, we report a neuronal circuit that allows Drosophila larvae to overcome amino acid deprivation and pupariate. We find that nutrient stress is sensed by the class IV multidendritic cholinergic neurons. Through live calcium imaging experiments, we show that these cholinergic stimuli are conveyed to glutamatergic neurons in the ventral ganglion through mAChR. We further show that IP3R-dependent calcium transients in the glutamatergic neurons convey this signal to downstream medial neurosecretory cells (mNSCs). The circuit ultimately converges at the ring gland and regulates expression of ecdysteroid biosynthetic genes. Activity in this circuit is thus likely to be an adaptation that provides a layer of regulation to help surpass nutritional stress during development.

Article and author information

Author details

  1. Siddharth Jayakumar

    National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
    Competing interests
    The authors declare that no competing interests exist.

  2. Shlesha Richhariya

    National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
    Competing interests
    The authors declare that no competing interests exist.

  3. O Venkateswara Reddy

    National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
    Competing interests
    The authors declare that no competing interests exist.

  4. Michael J Texada

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2479-1241

  5. Gaiti Hasan

    National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
    For correspondence
    gaiti@ncbs.res.in
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7194-383X

Funding

National Centre for Biological Sciences (Core funding)

  • Gaiti Hasan

Council of Scientific and Industrial Research (Graduate fellowship)

  • Siddharth Jayakumar

National Centre for Biological Sciences (Graduate fellowship)

  • Shlesha Richhariya

Howard Hughes Medical Institute

  • Gaiti Hasan

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Leslie C Griffith, Brandeis University, United States

Version history

  1. Received: May 4, 2016
  2. Accepted: August 4, 2016
  3. Accepted Manuscript published: August 5, 2016 (version 1)
  4. Accepted Manuscript updated: August 8, 2016 (version 2)
  5. Version of Record published: August 22, 2016 (version 3)

Copyright

© 2016, Jayakumar et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Siddharth Jayakumar
  2. Shlesha Richhariya
  3. O Venkateswara Reddy
  4. Michael J Texada
  5. Gaiti Hasan
(2016)
Drosophila larval to pupal switch under nutrient stress requires IP3R/Ca2+ signalling in glutamatergic interneurons
eLife 5:e17495.
https://doi.org/10.7554/eLife.17495

Share this article

https://doi.org/10.7554/eLife.17495

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