(A) Relative pre-rRNA (47S) levels were detected by RT-qPCR from 3T3-L1 fibroblast and fully differentiated adipocytes stimulated by switching culture medium from 1% BSA in PBS to full growth medium with insulin (Fed). (B) Basal (KRP buffer with 1% BSA) or 45 min stimulated (Fed: high glucose DMEM, 10% FBS, and insulin) pre-rRNA levels were detected by RT-qPCR in primary adipocytes isolated from wild type (WT) and PTRF null (KO) mice. (C) A stimulation time-course of B. (D) Pre-rRNA levels were detected by RT-qPCR from basal or stimulated wild type (WT), PTRF null (KO), PTRF re-transfected PTRF null (KO+PTRF), and Cav-1 re-transfected PTRF null (KO+Cav1) MEFs cells. (E) Relative levels of total proteins, RNA, 18S, and 47S from wild type or PTRF null mice (n = 6) were measured and normalized to cell numbers. *p<0.05, **p<0.01, and ***p<0.001; Student’s test. Error bars indicate SD.