Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
- Brandeis University, United States
- University of Lausanne, Switzerland
- Harvard University, United States
- University of Miami, United States
Figures
Figure 1
IR93a is expressed in Dorsal Organ Cool Cells (DOCCs) and is required for cool avoidance.
(a) Gene structure of the Ir93a locus; sequences encoding the transmembrane (TM) domains and channel pore are colored. The blue triangle denotes site of MiMIC insertion in Ir93aMI05555, and the …
Figure 2
Cool-responsive calcium and voltage changes in DOCCs require IR93a.
(a) Left: DOCC responses monitored using R11F02>GCaMP6m. DOCC cool-responsive increases in fluorescence are dramatically reduced in Ir93aMI05555, and responses are rescued by expression of a …
Figure 3
IR93a is co-expressed with IR25a and IR40a in sacculus neurons.
(a) Left: schematic of the adult Drosophila antenna, illustrating the location of the sacculus (red) in the interior of this appendage. Right: the sacculus is composed of three main chambers (I, II, …
Figure 4 with 1 supplement
Hygrosensory behavior requires IR93a, IR25a and IR40a.
(a) Schematic of the hygrosensory behavior assays. ~67% to ~96% RH gradients were generated by filling wells with either a saturated solution of ammonium nitrate in water or pure water. ~89% to ~96% …
Figure 4—figure supplement 1
Description of Ir40a mutants and analysis of Gal4-independent transgene expression.
(a) Gene structure and sequence alterations in Ir40a alleles. Regions encoding transmembrane domains (TMs) and pore region are in red. The Ir40a promoter region present in Ir40a-Gal4 is indicated in …
Figure 5
IR-dependent physiological responses to dry air.
(a) Schematic of the Drosophila head (viewed from above) illustrating the projection of IR40a/IR93a/IR25a-expressing neurons (green) (labeled using Ir40a-Gal4 [Silbering et al., 2011]) from the …
Figure 6
The TRP channels Nanchung and Water witch do not mediate IR-dependent dry sensation.
(a) Dry preference assessed on ~67% to ~96% gradient. Asterisks denote statistically different responses from wild type (**p<0.01; Steel with control). wild type, n = 16 assays. nan mutant (nan36a), …
Author response image 1
Ammonia increases the evaporative cooling effect of water.A temperature probe (Almemo Pt100, ZA9030-FS2) was dipped into water or 3% ammonia and immediately removed.
The temperature of the probe was recorded at 1 Hz during 10 s before and 50 s after dipping. The arrowhead indicated the time of dipping into the solution. Traces show temperature changes (mean±SEM; …
