(A) Cartoon representation of the RecBCD complex within a transparent surface showing the electron density. The RecB subunit is in orange, RecC in cyan, RecD is in green, and the DNA is in pink, (B) …
(A) An example of an aligned and summed image stack showing particle distribution. (B) Selection of 2D class averages representing different particle views. (C) Final map coloured by local …
(A) Electron density corresponding to the bound DNA fork substrate, (B) Representative regions at the centre of the RecBCD complex to illustrate the quality of the maps, (C) The 2B/SH3 domain of the …
Inset: location of the close ups shown in this figure relative to Figure 1. (A) Binding of the 5’-tail of the DNA fork across the RecD subunit, (B) Close up of the electron density (contoured at 4 σ)…
Insets show location of the zoomed in regions. (A) Conformational changes of the 1A and 2A domains of RecB associated with nucleotide binding (initiation complex (Singleton et al., 2004) shown in …
Surprisingly, only very weak electron density for bound nucleotide is visible at the ATP-binding site of RecD even though ADPNP is bound at the ATP-binding site of the RecB subunit. However, the …
Inset: location of the close ups shown in this figure relative to Figure 1. (A) Contact regions of the SH3 domain (blue) with DNA (pink) and peptide (RecB, orange), (B) Cartoon representation of the …
Inset: location of the close ups shown in this figure. The series of conformational changes from the initiation complex (grey) begins in the top left corner. Supplementary Videos 1–3 show these …
Inset shows location of the zoomed in region. (A) Electron density (contoured at 2.5 σ) showing lack of density for the nuclease blocking helix in the position seen in the initiation complex (grey). …
Refinement of the final model.
Final particles | 74656 |
Resolution (Å) | 3.8 |
CC (whole map) | 0.838 |
CC (around atoms) | 0.875 |
R.m.s. deviations | |
Bond lengths (Å) | 0.01 |
Bond angles (°) | 1.04 |
Ramachandran | |
Favoured (%) | 94.1 |
Outliers | 8/2874 |
Rotamers | |
Favoured (%) | 99.1 |
Outliers | 1/2426 |