Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

  1. Nidhi Sharma Dey
  2. Parvathy Ramesh
  3. Mayank Chugh
  4. Sudip Mandal
  5. Lolitika Mandal  Is a corresponding author
  1. Indian Institute of Science Education and Research Mohali, India
  2. University of Tuebingen, Germany

Abstract

Drosophila hematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) in Drosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identified Notch expressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship between Notch expressing HSCs and the previously described Domeless expressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).

Article and author information

Author details

  1. Nidhi Sharma Dey

    Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India
    Competing interests
    The authors declare that no competing interests exist.
  2. Parvathy Ramesh

    Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India
    Competing interests
    The authors declare that no competing interests exist.
  3. Mayank Chugh

    Cellular Nanoscience, Center for Plant Molecular Biology, University of Tuebingen, Tübingen, Germany
    Competing interests
    The authors declare that no competing interests exist.
  4. Sudip Mandal

    Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India
    Competing interests
    The authors declare that no competing interests exist.
  5. Lolitika Mandal

    Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India
    For correspondence
    lolitika@iisermohali.ac.in
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7711-6090

Funding

WellcomeTrust DBT Alliance (500124/Z09/Z)

  • Lolitika Mandal

Indian Institute of Science Education and Research Mohali

  • Nidhi Sharma Dey
  • Parvathy Ramesh
  • Mayank Chugh
  • Sudip Mandal
  • Lolitika Mandal

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Yukiko M Yamashita, University of Michigan, United States

Version history

  1. Received: May 30, 2016
  2. Accepted: October 25, 2016
  3. Accepted Manuscript published: October 26, 2016 (version 1)
  4. Accepted Manuscript updated: October 31, 2016 (version 2)
  5. Version of Record published: November 23, 2016 (version 3)
  6. Version of Record updated: November 30, 2016 (version 4)
  7. Version of Record updated: September 11, 2019 (version 5)

Copyright

© 2016, Dey et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 4,582
    Page views
  • 928
    Downloads
  • 35
    Citations

Article citation count generated by polling the highest count across the following sources: Scopus, Crossref, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Nidhi Sharma Dey
  2. Parvathy Ramesh
  3. Mayank Chugh
  4. Sudip Mandal
  5. Lolitika Mandal
(2016)
Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila
eLife 5:e18295.
https://doi.org/10.7554/eLife.18295

Further reading

    1. Stem Cells and Regenerative Medicine
    Lampros Mavrommatis, Hyun-Woo Jeong ... Holm Zaehres
    Research Article

    In vitro culture systems that structurally model human myogenesis and promote PAX7+ myogenic progenitor maturation have not been established. Here we report that human skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm and neuromesodermal progenitors and develop into organized structures reassembling neural plate border and dermomyotome. Culture conditions instigate neural lineage arrest and promote fetal hypaxial myogenesis toward limb axial anatomical identity, with generation of sustainable uncommitted PAX7 myogenic progenitors and fibroadipogenic (PDGFRa+) progenitor populations equivalent to those from the second trimester of human gestation. Single-cell comparison to human fetal and adult myogenic progenitor /satellite cells reveals distinct molecular signatures for non-dividing myogenic progenitors in activated (CD44High/CD98+/MYOD1+) and dormant (PAX7High/FBN1High/SPRY1High) states. Our approach provides a robust 3D in vitro developmental system for investigating muscle tissue morphogenesis and homeostasis.