(A) Metagene analysis of ribosome profiling data. Average ribosome read density profiles over well-expressed genes, aligned at their start codon and stop codons. Data from uninfected, 2, 4 and 8 hpi samples, and harringtonine pretreated 8 hpi sample. (B) Ribosome occupancies following treatments (illustrated on the left) with cycloheximide (CHX, top panels), and harringtonine (Harr, bottom panels) of the Influenza NS1 genomic locus at 8 hpi. Data from the plus strand (dark blue) and the minus strand (light blue) is presented. (C) Ribosome footprints profile of one human gene (ISG15) at 8 hpi. (D) Meta gene analysis from start codon for footprints originating from viral mRNAs. Data from 2, 4 and 8 hr of IAV infected and 8 hpi-harringtonine treated cells. (E) Fragment length organization similarity score (FLOSS) analysis for human mRNAs and for viral reads originating from the plus and minus strands.