(A) Field sign maps from six mice, illustrating differences between mouse lines and individual mice. The mouse line and duration of imaging are indicated on each map. Scale bar 0.5 mm. (B) Mean field sign maps for 4 Emx1-Ai96 and 10 Emx1-Ai93 mice, from 30–75 min of imaging. (C) Mean of the Emx1-Ai96 and Emx1-Ai93 field sign maps in panel B, with borders and area labels. (D) Map of variance of the visual field sign. Variance was calculated from visual field sign maps from 14 mice, after alignment as described for calculation of the mean field sign map. Whiter areas denote higher variance. Area borders are overlaid in white. (E) The probability of mapping different visual areas with GCaMP6 fluorescence in awake mice. Blue and red bars denote areas with negative and positive field signs, respectively. Results were derived from 14 mice (4 Emx1-Ai96, 10 Emx1-Ai93). Mouse numbers: V1 14/14, LM 14/14, LI 10/10, AL 14/14, LLA 10/13, RL 14/14, RLL 6/14, AM 13/14, PM 14/14, MMA 14/14, MMP 14/14, M 5/9, P 14/14, POR 1/3, where, for each area, the denominator indicates the number of mice in which the area was visible within the cranial window, determined manually. (F) Mean ± SEM fluorescence change for each visual area, derived from the △F/F spectral power. For each map power was normalized to that in V1. S1 region was drawn manually towards the anterior extent of the cranial window. Mouse numbers: LM 14, LI 10, AL 14, LLA 10, RL 14, RLL 5, AM 12, PM 14, MMA 12, MMP 12, M 5, P 14, S1 14; from 14 mice (4 Emx1-Ai96, 10 Emx1-Ai93).