Recruitment of inhibition and excitation across mouse visual cortex depends on the hierarchy of interconnecting areas
- Washington University School of Medicine, United States
- Allen Institute for Brain Science, United States
Figures
Figure 1 with 3 supplements
Hierarchy between V1, LM, and PM.
(a) Rostrocaudal series of coronal slices through the left hemisphere showing anterogradely labelled axonal projections (yellow/orange) after V1 was injected with BDA. Retrogradely labelled …
Figure 1—figure supplement 1
Darkfield images of the termination patterns of BDA-labelled axonal projections from V1 to LM, LI, P, POR, AL, PM, RL, AM and A.
Images are taken from the boxed regions shown in 1A. All projections are FF, which target L2-4 more strongly than L1. Scale bar, 0.5 mm.
Figure 1—figure supplement 2
Coronal sections showing anterogradely labelled axons (yellow/orange) from LM to V1, LI, P, POR, AL, PM, RL, AM and A, upon BDA injection into LM.
Calossally projecting neurons (light cyan) are labelled retrogradely after injection of bisbenzimide into the opposite hemisphere. Boxed regions show projections to each of the nine target areas. …
Figure 1—figure supplement 3
Axonal projections (yellow/orange) from PM to V1, LM, LI, P, POR, AL, RL, AM and A, upon BDA injection into PM.
Calossally projecting neurons (light blue, labeled with bisbenzimide) provide landmarks for areal identification (Wang and Burkhalter, 2007). Boxed regions show projections to target areas. Asterisk …
Figure 2 with 2 supplements
Subcellular ChR2-assisted mapping of V1→PM connections to L2/3 PV and Pyr cells.
(a) Coronal slices showing injection (left) and target (right) sites two weeks after the injection of AAV2/1.CAG.ChR2-Venus.WPRE.SV40 into V1. Scale bar, 500 µm. Select target areas indicated in …
Figure 2—figure supplement 1
V1→PM pathway in a PV-tdT mouse.
(a−e) Image of coronal section two weeks after the injection of AAV2/1.CAG.ChR2-Venus.WPRE.SV40 into V1. Slice includes areas PM, AL, and V1. PV cells in red (a,c), ChR2-Venus-expressing axons in …
Figure 2—figure supplement 2
Paired recordings of excitation-dependent, PV cell-mediated inhibition of Pyr cells.
(a) Coronal section through V1 and PM of a PV-Cre × Ai9 mouse in which PV cells express tdT (red). Scale bar, 200 µm. Inset: Higher magnification of the boxed region shows a high density of …
Figure 3 with 1 supplement
Lower I/E balance in PM→V1 pathway.
(a) Coronal slices showing AAV2/1.CAG.ChR2-Venus (green) injection in PM (top) and axonal labelling in target areas (bottom) of a PV-tdT (red) mouse. Scale bar, 1 mm. (b) EPSCssCRACM in a pair of …
Figure 3—figure supplement 1
Analyses of inputs to L2/3 PV and Pyr neurons in the reciprocal pathways between V1 and PM.
(a) The EPSC per 75 × 75 µm pixel, calculated as pA/µm (2), is larger in PV than in Pyr cells in FFV1→PM. (b) Individual PV cells receive interareal FFV1→PM input over a larger area than Pyr cells …
Figure 4 with 2 supplements
Interareal recruitment of L2/3 PV cells depends on the pathway and the hierarchical distance between areas.
(a) EPSCssCRACM in a L2/3 PV (left) and Pyr (right) cell in the FFLM→PM pathway. (b) sCRACM map of EPSCs in 5a with reconstructed neuron positions. (c) Scatter plot of all PV-Pyr cell pairs in the …
Figure 4—figure supplement 1
Stronger input to L1 in the PM-to-LM than in the LM-to-PM pathway.
(a) Normalized heat maps of average EPSCs in L2/3 PV cells in the FFLM→PM and FBPM→LM pathways. EPSC values normalized to peak pixel in each panel. (b) Interareal input to L1 is stronger for PV …
Figure 4—figure supplement 2
Comparisons of strengths, extent, and rise times of inputs in L2/3 pathways.
(a) Total EPSC per row plotted against row position for L2/3 PV and Pyr cells in the FFV1→PM, FFLM→PM, FBPM→LM and FBPM→V1 pathways. Strongest EPSCs in both cell types are observed in the LM→PM …
Figure 5
FF input to L5 neurons.
(a) FFV1→PM EPSCssCRACM in a pair of neighboring L5 PV (left) and Pyr (right) cells. (b) Heat map of EPSCs in 6a superimposed with respective biocytin-filled L5 neurons. (c) Scatter plot, as …
Figure 6
FB input to L5 neurons.
(a) FBPM→LM EPSCssCRACMin a pair of neighboring L5 PV (left) and Pyr (right) cells. (b) Heat map of EPSCs from 7a superimposed with the respective biocytin-filled L5 neurons. (c) Scatter plot of all …
