Smad4 restricts differentiation to promote expansion of satellite cell derived progenitors during skeletal muscle regeneration
- University of Rochester Medical Center, United States
Figures
Figure 1
Loss of Smad4 expression in aged satellite cells and myogenic progenitors during muscle regeneration.
Representative profiles of SMAD4 protein levels by flow cytometric analysis in SCs and MPs isolated from C57BL6 (A) adult and (B) aged uninjured and 5-day post injured (5dpi) (TA) muscles. (C) …
Figure 2 with 1 supplement
Disruption of Smad4 expression in P7:S4KO satellite cells and myogenic progenitors during muscle regeneration.
Representative profiles of SMAD4 protein levels by flow cytometric analysis in SCs and MPs isolated from adult (A) Ctl and (B) P7:S4KO uninjured and 5dpi TA muscles. (C) Quantification of SMAD4 …
Figure 2—figure supplement 1
Gating of control and P7:S4KO SCs for intracellular protein analysis.
Representative flow cytometry plots of Ctl and P7:S4KO SCs used to examine SMAD4 protein levels in Figure 2.
Figure 3 with 1 supplement
Enhanced terminal myogenic commitment in P7:S4KO satellite cells and myogenic progenitors.
(A) Representative images of Pax7 (red), Myogenin (green), and DAPI (blue) immunofluorescence in FACs-sorted adult Ctl or P7:S4KO 96 hr SC cultures (plated at 4000 cells/well) treated with vehicle, …
Figure 3—figure supplement 1
Modulations in FGFR and FGF expression following SC-specific Smad4 loss in adult mice.
Quantification of (A) Fgf1, (B) Fgf2, (C) Fgf6, (D) Fgfr1, and (E) Fgfr4 expression in FACs-purified adult SCs and MPs cultured for five days. N = 3 mice, *p<0.05 t-test.
Figure 4
Reduced proliferation in P7:S4KO satellite cells and myogenic progenitors.
(A) Representative images of MyoD (green), EdU (grey), and DAPI (blue) immunofluorescence of FACs-sorted adult Ctl or P7:S4KO 72 hr SC cultures pulsed with EdU for the last 4 hr. (B) Quantification …
Figure 5 with 2 supplements
Smad4 disruption induces loss of proliferating and total satellite cell number during muscle regeneration.
(A) Representative images of Pax7 (red), BrdU (green), DAPI (blue) and Laminin (grey) immunofluorescence in adult 5dpi Ctl and P7:S4KO TA muscle sections. (B) Quantification of the proportion of …
Figure 5—figure supplement 1
Smad4 disruption does not induce Cdkn1a, Cdkn1b, or Cdkn2a expression in SCs and MPs sorted from adult regenerating TA muscle.
Quantification of (A) Cdkn1a (p21) (B) Cdkn1b (p27), and (C) Cdkn2a (p16) expression in SCs and MPs FACs-sorted from adult 5dpi Ctl and P7:S4KO TA muscles. N = 3 mice, *p<0.05 t-test.
Figure 5—figure supplement 2
Smad4 disruption does not induce loss of Pax7+ SCs in uninjured TA muscles.
(A) Representative images of Pax7 (red), DAPI (blue) and Laminin (grey) immunofluorescence of adult uninjured Ctl and P7:S4KO TA muscle sections. (B) Quantification of Pax7+ cells in adult uninjured …
Figure 6
Smad4 disruption enhances terminal myogenic commitment during muscle regeneration.
(A) Representative images of active Caspase 3 (aCasp, green), MyoD (red), DAPI (blue), and Laminin (grey) immunofluorescence of adult 5dpi Ctl and P7:S4KO TA muscle sections. MyoD+ cells (white …
Figure 7 with 1 supplement
Smad4 disruption in satellite cells impairs skeletal muscle regeneration.
Representative images of embryonic Myosin Heavy Chain (eMyHC, green), DAPI (blue) and Laminin (grey) immunofluorescence of 5dpi Ctl and P7:S4KO (A) adult and (B) aged TA muscle sections. (C) …
Figure 7—figure supplement 1
Smad4 disruption reduces Smad4 and the SMAD target Id1 expression in adult and aged SCs and MPs sorted from regenerating TA muscle.
Quantification of (A) Smad4, (B) Id1 and (C) Cdkn2a (p16) mRNA levels in SCs and MPs FACs-sorted from 5dpi adult and aged Ctl and P7:S4KO TA muscle sections. N = 3 mice, *p<0.05 ANOVA Fishers test.
Figure 8
Smad4 disruption in satellite cells leads to persistent deficits in skeletal muscle regeneration.
Representative images of H and E-stained uninjured and 14dpi, and Sirius Red-stained 14dpi (A) adult and (B) aged TA muscle sections. Quantification and frequency distribution of (C) uninjured and (D…
