(A) Pdmsr-1:HisCl worms were placed on histamine-containing agar, and sleep was induced by ultraviolet C (UVC) light irradiation. Worms were compared to the wild type strain (N2) on histamine, as well as to worms not exposed to histamine. Gray lines indicate control worms that were not exposed to UVC light, whose quiescence was not affected by activation of HisCl channels. Statistical comparisons were performed using a 2-way repeated measures ANOVA using time and experimental group as factors. Asterisks indicate lowest level of significance in comparisons between Pdmsr-1:HisCl on histamine with each of the other groups in pairwise comparison using Bonferroni correction. *p<0.05, **p<0.005, ***p<0.0005. (B) Total amount of sleep over eight hours from Figure 6A for each individual worm. There was a significant interaction between histamine and HisCl channels such that HisCl channels only increased sleep in the presence of histamine. ***p<0.0005. There were no significant effects found for worms that were not irradiated with UV. NS denotes not significant. (C) Total activity analysis across an eight-hour period. HisCl channels reduced activity only in animals exposed to histamine, and there were no significant effects for worms that were not irradiated with UV. ***p<0.0005. NS denotes not significant. In all three panels, triangles represent animals that expressed Pdmsr-1:HisCl, circles represent animals that do not express Pdmsr-1:HisCl, filled symbols represent animals that have been exposed to histamine, empty symbols represent animals that have not been exposed to histamine, black symbols represent animals that have been exposed to UV irradiation, and gray symbols represent animals that were not exposed to UV irradiation. In panels B and C, statistical significance was assessed with a 2×2 Factorial ANOVA with Bonferroni post-hoc correction.