Mg2+ regulates many physiological processes and signalling pathways. However, little is known about the mechanisms underlying the organismal balance of Mg2+. Capitalizing on a set of newly generated mouse models, we provide an integrated mechanistic model of the regulation of organismal Mg2+ balance during prenatal development and in adult mice by the ion channel TRPM6. We show that TRPM6 activity in the placenta and yolk sac is essential for embryonic development. In adult mice, TRPM6 is required in the intestine to maintain organismal Mg2+ balance, but is dispensable in the kidney. Trpm6 inactivation in adult mice leads to a shortened lifespan, growth deficit and metabolic alterations indicative of impaired energy balance. Dietary Mg2+ supplementation not only rescues all phenotypes displayed by Trpm6-deficient adult mice, but also may extend the lifespan of wildtype mice. Hence, maintenance of organismal Mg2+ balance by TRPM6 is crucial for prenatal development and survival to adulthood.https://doi.org/10.7554/eLife.20914.001
A balanced diet contains a variety of minerals such as magnesium ions, which are required for many chemical reactions in our body. A shortage of magnesium ions is linked to many diseases and is thought to be especially harmful to babies in the womb and shortly after birth. Magnesium ion deficiency is widespread in human populations and in the US is thought to affect up to 68% of people.
Despite its prominent role in human health, our understanding of how the body maintains the right balance of magnesium ions remains extremely vague. Magnesium ions can enter and leave a cell by passing through specific types of proteins that form channels in the membrane surrounding the cell. There are thought to be around ten types of these magnesium ion channels in human cells, but we do not know what roles any of them perform in the body. One such channel called TRPM6 may be particularly important because mutations in the gene that encodes this channel can cause magnesium ion deficiency in human infants. However, the loss of TRPM6 in mice disrupts how mouse embryos develop, suggesting that our current view on the role that TRPM6 plays in regulating the magnesium ion balance in humans may be too simplistic.
To address this question, Chubanov et al. studied mice with mutations that disrupted the production of TRPM6 in specific tissues only. The experiments show that TRPM6 primarily operates in the placenta and intestine to regulate the balance of magnesium ions in the body. Further experiments show that the loss of TRPM6 in adult mice leads to reduced lifespan, growth defects and poor health by disrupting important biochemical reactions. Supplying the mutant mice with magnesium ion supplements improved their health and could extend lifespans of normal animals.
The findings of Chubanov et al. demonstrate that TRPM6 plays a crucial role in regulating the levels of magnesium ions in mice before birth and into adulthood. The next step is to carry out large-scale experiments to investigate the effects of altering the levels of magnesium ions in human diets.https://doi.org/10.7554/eLife.20914.002
Mg2+ is the most abundant intracellular divalent cation and is essential for the regulation of a broad spectrum of metabolic and signalling pathways (de Baaij et al., 2015). In addition, direct association with Mg2+ fosters the structural integrity of key metabolites (such as ATP), proteins, lipid membranes and nucleic acids (de Baaij et al., 2015) implying that organismal Mg2+ deficiency, a surprisingly common condition in humans (King et al., 2005; Rosanoff et al., 2012), may be especially harmful during prenatal development and early postnatal life, when the production of and the demand for Mg2+-bound metabolites is particularly high. There is growing evidence to suggest that Mg2+ deprivation is accompanied by different types of metabolic, immune, cardiovascular and neurological disorders (de Baaij et al., 2015). However, mainly due to the lack of adequate mammalian genetic models, it still remains unclear whether an imbalance in Mg2+ metabolism is merely associated with or can directly trigger the latter pathophysiological processes. Furthermore, it has recently been shown that cellular Mg2+ fluxes regulate the circadian rhythm and energy balance (Feeney et al., 2016), CGRP-mediated osteogenic differentiation (Zhang et al., 2016) and synaptic plasticity (Palacios-Prado et al., 2014), and that changes in the composition of brain interstitial Mg2+ concentrations participate in the control of the sleep-wake cycle (Ding et al., 2016).
The remarkable recent progress in our understanding of the critical role of Mg2+ in health and disease contrasts with the dearth of knowledge about the mechanisms governing cellular and organismal Mg2+ balance. Approximately 10 plasma membrane Mg2+ channels have been proposed (Quamme, 2010) indicating a high degree of redundancy. However, quite some controversy surrounds the biological role of many of these proteins, and the question whether there is a central gatekeeper responsible for organismal Mg2+ balance has not yet been answered. The kinase-coupled ion channel TRPM7 has been proposed as a ubiquitous, indispensable cellular Mg2+ entry pathway (Schmitz et al., 2003; Chubanov et al., 2004; Ryazanova et al., 2010; Stritt et al., 2016). However, studies with Trpm7 gene-deficient mice failed to confirm a corresponding in vivo role of Trpm7. Thus, constitutive inactivation of Trpm7 in mice entailed early embryonic lethality for as yet unknown reasons (Jin et al., 2008). Furthermore, tissue-specific deletions of Trpm7 in mouse embryos affected morphogenesis of internal organs apparently in a Mg2+-independent manner (Jin et al., 2008, 2012; Sah et al., 2013). More recently, it was suggested that the Mg2+ transporter MagT1 rather than TRPM7 might play a critical role for Mg2+ homeostasis in T lymphocytes (Li et al., 2011) and probably also in the whole embryo (Zhou and Clapham, 2009). Hence, the biological role of TRPM7 requires further clarification.
In the present work, we focussed on the closest TRPM7 relative, TRPM6, because loss-of-function mutations in TRPM6 cause hypomagnesemia (low Mg2+ blood levels) in human infants thought to mainly result from renal Mg2+ wasting (Schlingmann et al., 2002; Walder et al., 2002; Voets et al., 2004). However, deletion of Trpm6 in mice has resulted in neural tube closure defects and embryonic death (Walder et al., 2009) indicating a direct role of TRPM6 in developmental processes and calling into question the simplistic view on the human TRPM6 phenotype.
By integrating systematic phenotyping of Trpm6 gene-modified mice with biochemical analysis, gene expression, metabolomics, and cell biological approaches, we decipher the molecular and organismal roles of TRPM6 in prenatal development and postnatal survival.
To understand the role of Trpm6 in prenatal development, we determined the onset of embryonic lethality in Trpm6 null embryos and investigated the expression pattern of Trpm6 at this stage. Using a mouse strain carrying a gene-trap mutation in Trpm6 (Trpm6βgeo) (Table 1), we found that Trpm6βgeo/βgeo embryos were present at embryonic days (e) 8.5–10.5 (Figure 1A). However, only a few mutants were found between e11.5–12.5 and no Trpm6βgeo/βgeo individuals were viable after e14.5 (Figure 1A). Compared to e9.5 C-shaped Trpm6+/+ individuals, all Trpm6βgeo/βgeo embryos isolated had not turned (S-shaped) and were smaller indicating a developmental retardation after e8.5 (Figure 1B). Consequently, we investigated the expression pattern of Trpm6 in e8.5 fetuses by in situ hybridization (ISH) and found that Trpm6 was specifically expressed in the visceral yolk sac endoderm and extraembryonic chorion (Figure 1C) and that Trpm6 was not detectable in the neural tube (Figure 1—figure supplement 1). Within the placental labyrinth a network of maternal sinusoids are intertwined with fetal blood capillaries, separated by two layers of transporting trophoblast cells, syncytiotrophoblasts I (SynT-I) and II (SynT-II) (Simmons and Cross, 2005; Simmons et al., 2008). At e8.5, morphogenesis of the labyrinth is in the initial stages and SynT-I/SynT-II cell layers are distinguishable (Simmons and Cross, 2005; Simmons et al., 2008). We observed that Trpm6 expression was restricted to SynT-I cells (Figure 1D). In the fully maturated labyrinth at e14.5 Trpm6 mRNA was detected in syncytiotrophoblasts as well (Figure 1E).
Syncytiotrophoblasts and endoderm cells of the yolk sac exchange metabolites between the maternal and fetal blood (Simmons and Cross, 2005). To clarify whether TRPM6 is required for Mg2+ supply by extraembryonic tissues, we used inductively coupled plasma mass spectrometry (ICP-MS) and found that relative magnesium (Mg2+) levels were reduced in the whole e9.5 Trpm6βgeo/βgeo embryos (Figure 1F). Thus, Trpm6 is specifically expressed in the placental labyrinth and the yolk sac at the stage when the Mg2+ deficiency and growth delay of Trpm6-deficient embryos become apparent.
To investigate whether TRPM6 activity in extraembryonic cells underlies the lethality of Trpm6 null embryos, we characterized a mouse strain with a ‘floxed’ (Trpm6fl) allele (Table 1). Cre-mediated excision engendered viable mice heterozygous for the constitutive deletion mutation in Trpm6 (Trpm6Δ17/+). However, we were unable to produce live Trpm6βgeo/Δ17 or Trpm6Δ17/Δ17 offspring, indicating that Trpm6∆17 is a true null mutation (Table 1). The paternally inherited Sox2-Cre transgene drives recombination only in epiblast cells, but not in extraembryonic tissues (Hayashi et al., 2003). Notably, intercrosses of Trpm6Δ17/+;Sox2-Cre males and Trpm6fl/fl females resulted in viable Trpm6Δ17/Δ17 pups at the expected ratio (Table 1). Therefore, the embryonic mortality of Trpm6-deficient mice appears to be caused by the loss of TRPM6 in extraembryonic tissues.
We next studied the impact of a global deletion of Trpm6 postnatally. Examination of Trpm6-deficient (Trpm6Δ17/Δ17;Sox2-Cre) mice at weaning did not reveal conspicuous abnormalities. However, during the follow-up period, we observed the gradual development of pathologies. Thus, Trpm6-deficient mice had a lifespan of no longer than 16 weeks (Figure 2A). Mutants were growth-delayed, and displayed a lighter fur colour and low night-time activity (Figure 2B,C). Weight gain and lean body mass of Trpm6-deficient mice were reduced (Figure 2D,E), as was the muscle fibre area of the gastrocnemius muscle of 12–13 week-old Trpm6-deficient mice indicative of sarcopenia (Figure 2F). Mutant mice displayed kyphosis (Figure 2G) and completely lacked abdominal and subcutaneous fat depots (Figure 2H,I) indicative of catabolic metabolism. However, the total amount of faeces (Figure 2—figure supplement 1A) and the calorimetrically determined faecal energy content, as a measure of energy excretion (Figure 2—figure supplement 1B), were not altered in Trpm6 mutants, ruling out insufficient food intake. Histological analysis of internal organs (Figure 3) showed that Trpm6-deficient mice developed lung emphysema and degeneration of lymphoid organs. Thus, the thymus of mutant mice was rudimentary and the cortex region was not distinguishable. In the spleen of Trpm6-deficient mice, the red pulp was substantially reduced. Hepatocytes of Trpm6-deficient mice were depleted of glycogen granules (Figure 3), corroborating catabolic metabolism. It has been suggested that low serum Mg2+ and TRPM6 function are associated with atherosclerosis in humans (Maier, 2012; Tin et al., 2015). Therefore, we investigated whether such a phenotype would develop in our mouse model as well. However, examination of thoracal aorta showed no signs of atherosclerosis development in mutant mice (Figure 2—figure supplement 2).
Next, we asked whether the phenotype of Trpm6-deficient mice is caused by Mg2+ deficiency. We employed ICP-MS to compare the concentrations of main elements in serum from controls and Trpm6-deficient littermates. We found that, similar to humans with mutations in the TRPM6 gene (Schlingmann et al., 2002; Walder et al., 2002), Trpm6-deficient mice developed hypomagnesemia (Figure 2J). Serum Mg2+ levels of mutant mice were only 0.58 mM (36% of control value, 1.58 mM), whereas concentrations of other elements were not changed (Figure 2J). A Mg2+-enriched diet is an efficient way to alleviate hypomagnesemia in humans lacking TRPM6 (Schlingmann et al., 2002; Walder et al., 2002). Therefore, we asked whether the phenotypes of Trpm6-deficient mice were caused by Mg2+ deprivation and could be rescued by dietary supplementation. To this end, we changed the regular chow (0.22% Mg2+) of 4 week-old mutant mice and control littermates for a Mg2+ enriched diet (0.75% Mg2+). Notably, none of the Trpm6-deficient mice died during the following 12 weeks of Mg2+ supplementation (Figure 2K). However, returning to regular chow resulted in 100% mortality of mutant mice within the following 13 weeks (Figure 2K). Mg2+ supplemented mutants neither exhibited kyphosis nor lipodystrophy (data not shown). Furthermore, the morphology of the lung, spleen, and thymus of Mg2+ supplemented mutants closely resembled that of control mice (Figure 3). We asked whether dietary Mg2+ supplementation of Trpm6βgeo/+ parents would benefit the survival of Trpm6-deficient offspring. However, similar to a previous study (Walder et al., 2009), we found that this treatment was inefficient.
Trpm6-deficient adult mice phenocopy salient pathologies reported for a set of mouse strains advocated as genetic models of ‘accelerated’ or ‘premature’ aging (Kuro-o et al., 1997; Trifunovic et al., 2004; Kujoth et al., 2005; Varela et al., 2005; Mostoslavsky et al., 2006; Niedernhofer et al., 2006; van der Pluijm et al., 2007; López-Otín et al., 2013). Similar to Trpm6-deficient mice, the latter mutants display short lifespan, growth failure, low physical activity, kyphosis, lung emphysema, sarcopenia, lipodystrophy and degeneration of lymphoid organs. A characteristic feature of these mouse strains is suppression of the somatotropic axis accompanied by induction of xenobiotic detoxification gene networks in the liver (Niedernhofer et al., 2006; van de Ven et al., 2006; van der Pluijm et al., 2007; Schumacher et al., 2008; Garinis et al., 2009; Mariño et al., 2010), interpreted as a defensive organismal response, slowing down growth and metabolism in favor of somatic preservation (López-Otín et al., 2013). We asked whether Trpm6-deficient mice would also display such protective metabolic responses. In fact, we found that serum IGF1 concentrations were reduced in Trpm6-deficient mice as well (Figure 4A). Mutant mice had a lower core body temperature (Figure 4B) and a profoundly reduced urinary content of major urinary proteins (MUPs) (Figure 4C), two known features of supressed IGF1 signalling (Mariño et al., 2010; Bartke et al., 2013). Even though mutant mice showed signs of overall energy shortage, circulating levels of ketone bodies (ß-hydroxybutyrate) were not elevated (Figure 2—figure supplement 1C). When subjected to an oral glucose tolerance test, mutant mice displayed lower peripheral glucose concentrations than controls despite of a similar amount of insulin released, thus reflecting increased insulin sensitivity (Figure 2—figure supplement 1D,E), another hallmark of suppressed IGF1 signalling (Bartke et al., 2013). Notably, body weight and IGF1 serum levels were indistinguishable in Mg2+ supplemented mutant and control mice suggesting that the variations observed in mice maintained on a regular diet were induced by Mg2+ deficiency (Figure 2—figure supplement 1F,G).
Finally, we performed whole-genome profiling of the liver transcriptome (Supplementary file 1). Applying a cut-off value of p≤0.1 for the false discovery rate (FDR), we identified 46 genes up- or down-regulated in the livers of Trpm6-deficient mice (Figure 4—figure supplement 1, Supplementary file 1). The majority of affected transcripts code for solute carrier transporters, cytochrome P450 metabolising enzymes, glutathione S-transferases and proteins metabolizing steroids. As expected, Ingenuity Pathway Analysis (IPA) revealed that the inactivation of Trpm6 is associated with an induction of interconnected gene networks controlling toxicity responses and xenobiotic metabolism governed by nuclear receptors such as retinoid X receptors (RXR), liver X receptor (LXR) and farnesoid X receptor (FXR) (Figure 4D, Supplementary file 2). Hence, Trpm6-deficient mice were characterized by suppression of the somatotropic axis and induction of xenobiotic detoxification responses.
To gain mechanistic insight into the altered energy metabolism of mutant mice, we quantified serum, liver and skeletal muscle levels of 237 metabolites (Supplementary file 3, Figure 4—figure supplement 2). Unexpectedly, alterations in Trpm6-deficient mice (FDR p≤0.1) were restricted to only several metabolites representing mainly long-chain acylcarnitines (AC), phosphatidylcholines (PC), and sphingomyelins (SM) (Figure 4E). These findings, as well as the results of gene array profiling (Figure 4D) suggest that sustained Mg2+ deficiency triggers a specific metabolic response rather than widespread unsystematic changes. In line with this idea, we noted that AC C18:1 and the related AC C18 were consistently increased in tissues of Trpm6-deficient mice (Figure 4F–J), whereas carnitine levels were not changed (Supplementary file 3). In conjunction with lowered concentrations of glucose and unchanged ketogenesis (Figure 2—figure supplement 1C), this constellation is a metabolic ‘signature’ of a frequent inherited human disorder characterized by inefficient β-oxidation of fatty acids due to mitochondrial carnitine palmitoyltransferase II deficiency (Gempel et al., 2002; Bonnefont et al., 2004). AC C18:1 and C18 levels were normalized in serum and tissues obtained from Mg2+ supplemented mutants (Figure 4F–J), implying that metabolic changes of AC were caused by Mg2+ deficiency.
Consequently, we asked whether Mg2+ would specifically affect mitochondrial ATP production (Figure 4K) and maintenance of the mitochondrial membrane potential (MMP) (Figure 4—figure supplement 3A). To address this question, wildtype liver mitochondria were incubated in a buffer containing 3 mM EDTA with or without 1–5 mM Mg2+. Such manipulations had no negative effect on mitochondrial respiration when succinate was offered as an energy source (Figure 4K, Figure 4—figure supplement 3A). In contrast, mitochondria failed to utilize octanoylcarnitine or palmitylcarnitine for ATP production (Figure 4K) and to maintain MMP (Figure 4—figure supplement 3A) in the presence of EDTA. Importantly, ATP production and MMP could be fully rescued by the administration of 3–5 mM Mg2+ (Figure 4K, Figure 4—figure supplement 3A), but not Zn2+ or Ca2+ (Figure 4—figure supplement 3B). Thus, sustained Mg2+ deprivation impairs energy homeostasis resulting in a catabolic metabolism in Trpm6-deficient mice, at least partially due to insufficient mitochondrial utilization of AC.
Next, we investigated the etiology of hypomagnesemia in Trpm6-deficient mice. ~50% of body Mg2+ content is stored in bones, ~30% in muscle tissues and only ~1% in the serum (de Baaij et al., 2015). Using ICP-MS we studied the Mg2+ content in bones (right tibia) and gastrocnemius muscle, and observed that Mg2+ levels in bones of Trpm6 null mice were only 24% of control values (Figure 5A). Furthermore, the Mg2+ content of muscle was also significantly reduced in Trpm6-deficient mice (Figure 5B). Hence, Trpm6-deficient mice develop a severe systemic Mg2+ deficit.
It is generally assumed that the distal convoluted tubule (DCT) of the kidney is critical for whole-body Mg2+ balance (de Baaij et al., 2015). Accordingly, immunostaining of control kidney cryosections with a TRPM6-specific antibody labelled nephron segments resembling DCT (Figure 5C). TRPM6 was not detectable in the kidneys of Trpm6-deficient mice. Surprisingly, mutant mice exhibited substantially reduced urinary Mg2+ excretion (only 14% of control values, Figure 5D), whereas fecal Mg2+ loss was significantly increased (166%, Figure 5E), indicating that mice lacking TRPM6 develop Mg2+ deficiency primarily due to impaired intestinal Mg2+ uptake. Therefore, we studied the expression pattern of Trpm6 in the intestine. Because the TRPM6-specific antibody did not efficiently and specifically detect TRPM6 protein in the intestine, we resorted to ISH (Figure 5—figure supplement 1). Trpm6 transcripts were not detectable in the small intestine, but Trpm6-specific signal was observed in absorptive epithelial cells of the colon (Figure 5—figure supplement 1). The colon of mutant mice was not stained by a Trpm6-specific ISH probe (Figure 5F), consistent with the notion that systemic Mg2+ deficit in Trpm6-deficient mice was primarily caused by a defect of Mg2+ uptake in the colon.
To directly assess the contribution of the kidney versus intestine to the Trpm6 null phenotype, we employed Ksp-Cre (Shao et al., 2002) and Villin1-Cre (Madison et al., 2002) transgenic mice to specifically ablate floxed Trpm6 alleles in renal and intestinal epithelial cells, respectively (Table 1). Surprisingly, conditional Trpm6 inactivation in the kidney neither impacted serum Mg2+ concentration nor urinary Mg2+ excretion, and bone Mg2+ content was only slightly reduced (Figure 5G–J). In contrast, disruption of Trpm6 in the intestine resulted in hypomagnesemia, reduced bone Mg2+ content and lowered urinary Mg2+ excretion (Figure 5K–N), indicating that wildtype kidneys are not able to compensate for the ablation of intestinal TRPM6. Hence, in contrast to current thinking, our findings support the new concept that Trpm6-dependent Mg2+ uptake in the intestine plays an indispensable role for systemic Mg2+ balance.
TRPM6 is invariably co-expressed with the TRPM7 channel and the question as to why TRPM6 function is non-redundant remains central to a mechanistic understanding of the Trpm6 null phenotype. Because Trpm6 expression levels in the epithelial cells of the colon (Figure 5—figure supplement 1) and the kidney (Figure 5C) are highly heterogeneous, we searched for an alternative native cell model to dissect the functional interplay of TRPM6 and TRPM7. Trophoblast stem (TS) cells are widely used to study the transport function of placental trophoblasts (Tanaka et al., 1998; Simmons and Cross, 2005) and, as shown before (Figure 1), this cell type is crucial for the fetal Trpm6 phenotype. Therefore, we derived Trpm6+/+ and Trpm6-deficient (Trpm6βgeo/βgeo) TS cells from e3.5 blastocysts isolated from Trpm6βgeo/+ parents (Figure 6—figure supplement 1A). We also produced Trpm7+/+ and Trpm7-deficient (Trpm7Δ17/Δ17) TS cells (Figure 6—figure supplement 2A) using Trpm7Δ17/+ mice (Jin et al., 2008). As expected, RT-PCR analysis revealed that wildtype TS cells expressed TRPM6 and TRPM7 (Figure 6—figure supplement 1B, Figure 6—figure supplement 2B). Trpm6βgeo/βgeo TS cells were maintained in culture for >40 passages. Furthermore, analysis of DNA content showed that the proportion of polyploidy was similar in Trpm6+/+ and Trpm6βgeo/βgeo TS cells (Figure 6—figure supplement 1C,D), suggesting that inactivation of Trpm6 did not affect the self-renewal of Trpm6βgeo/βgeo stem cells. In contrast, Trpm7Δ17/Δ17 TS cells did not proliferate, unless the cell culture medium was supplemented with additional Mg2+ (Figure 6—figure supplement 2C), supporting the concept that TRPM7 plays a pivotal role in cellular Mg2+ uptake that cannot be maintained by TRPM6 alone (Schmitz et al., 2003; Chubanov et al., 2004; Ryazanova et al., 2010).
TRPM6 and TRPM7 have been suggested as molecular correlates of MgATP- and Mg2+-regulated cation currents responsible for the cellular uptake of divalent cations including Mg2+ (Aarts et al., 2003; Nadler et al., 2001; Schmitz et al., 2003; Chubanov et al., 2004; Voets et al., 2004; Zhang et al., 2014). Patch-clamp analysis showed that Trpm6βgeo/βgeo TS cells display substantially reduced TRPM6/M7-like outward currents at +80 mV (Figure 6A). Due to permeation block by extracellular divalent cations (Nadler et al., 2001; Fleig and Chubanov, 2014), inward currents at physiological membrane potentials were very small (Figure 6A; p≤0.001, t-test). However, exposure of TS cells to a divalent cation free (DVF) solution resulted in large monovalent cation currents (Figure 6B). Under these conditions, mutant TS cells exhibited a comparable reduction of inward (p≤0.01, t-test) as well as outward (p≤0.05, t-test) monovalent currents (Figure 6B).
Cytosolic levels of free Mg2+ ([Mg2+]i) and MgATP ([MgATP]i) have been suggested to exert a negative feedback mechanism on TRPM6/M7 channel activity (Fleig and Chubanov, 2014). We observed (Figure 6C) that currents in Trpm6βgeo/βgeo TS cells were more susceptible to concentration-dependent inhibition by cytosolic MgATP (p≤0.0001, F-test). The calculated IC50 value for Trpm6+/+ was 3.17 mM (Hill (h) slope = −1.81). Currents in Trpm6βgeo/βgeo cells were inhibited by [MgATP]i with an IC50 value of 1.45 mM (h = −1.95; p≤0.0015, F-test). These results suggest that physiological concentrations of [MgATP]i varying between 2–7 mM in mammalian cells (Günther, 2006; Romani, 2011) will affect currents in Trpm6+/+ and Trpm6βgeo/βgeo cells differently. In contrast, we observed no significant differences in [Mg2+]i concentration-response curves for Trpm6+/+ and Trpm6βgeo/βgeo currents (p=0.62, F-test) (Figure 6D). The obtained IC50 value for Trpm6+/+ currents was 0.60 mM (h = −1.14) and was not significantly altered in Trpm6βgeo/βgeo TS cells (0.72 mM, h = −1.18; p=0.22, F-test), suggesting that physiological concentrations (0.3–1 mM, (Günther, 2006; Romani, 2011)) of [Mg2+]i will exert similar effects on ion currents in Trpm6+/+ and Trpm6βgeo/βgeo cells.
Next, we asked whether TRPM6 channel activity would be detectable in the absence of TRPM7. Remarkably, we observed that Trpm7Δ17/Δ17 TS cells completely lacked TRPM6/M7-like currents (Figure 6E,F). These findings cogently support our model (Chubanov et al., 2004) that native TRPM6 primarily functions in close cooperation with TRPM7. TRPM6 facilitates Mg2+ uptake by increasing the amplitude of TRPM7-like currents and relieving TRPM7 from the negative feedback by MgATP (Figure 6G).
Finally, we studied whether Mg2+ deprivation may affect energy metabolism at the cellular level. We chose the genetically tractable human haploid leukaemia cell line (HAP1 cells) (Essletzbichler et al., 2014; Blomen et al., 2015; Wang et al., 2015) as a new model system. CRISPR/Cas9-mediated ablation of the TRPM7 protein in HAP1 cells (Figure 6—figure supplement 3A,B) completely abolished TRPM7-like currents (Figure 6—figure supplement 3C). When cultured in standard medium for 24 hr, TRPM7-deficient cells were characterized by a reduced total cellular Mg2+ content and a Mg2+-dependent proliferation defect (Figure 6—figure supplement 3D,E). In addition, TRPM7-deficient HAP1 cells had reduced intracellular ATP levels (Figure 6—figure supplement 3F). Finally, the respiration rate of TRPM7-deficient HAP1 cells was significantly lower when compared to control cells (Figure 6—figure supplement 3G,H).
Our studies with Trpm6-deficient mice clearly demonstrated that a sustained disruption of Mg2+ homeostasis is detrimental for overall health and eventually reduces the lifespan of affected animals. Conversely, we asked whether a Mg2+-enriched diet might exert a beneficial effect on the lifespan of wildtype mice. Since there are no prior reports on lifespan extension of mice subjected to life-long dietary Mg2+ supplementation (or any other mineral), we performed proof-of-principle experiments to investigate, if such an effect can be observed. To this end, we used only the long-lived B6C3F1 hybrid mouse strain to avoid genotype-specific effects on disease susceptibility observed in longevity experiments with inbred strains (Lipman et al., 1999; Turturro et al., 1999; Mitchell et al., 2016). At this stage, we studied only females because of a larger number of early losses of males due to fighting (Miller et al., 2007). Finally, we studied animals only under pathogen-free conditions, since Mg2+ may elicit a protective effect via the immune system (Brandao et al., 2013; Chaigne-Delalande et al., 2013). Consistent with published reports (Turturro et al., 1999), the mean lifespan of B6C3F1 mice (886 days regarded as 100%) was significantly extended (1100 days, 125%) by dietary restriction (DR) (Figure 7A,B, Table 2). Remarkably, supplementation with three Mg2+ salts (Mg(CH3COO)2, Mg(OH)2 and MgCl2) increased the mean lifespan of mice by approximately 10% (976, 976 and 961 days, respectively). The nutritional CaCl2 administration was without any significant effect (828 days). In contrast to DR, animals supplemented with Mg2+ had a normal or even increased body weight (Figure 7C), ruling out the possibility that high dietary Mg2+ affected the lifespan of mice due to reduced food intake. Hence, opposite to Trpm6-dependent Mg2+ deprivation, dietary Mg2+ supplementation may have a beneficial effect on the lifespan of mice suggestive future large-scale longevity studies with varied conditions and species.
Here, we present a new mechanistic model of the regulation of Mg2+ homeostasis during development and postnatal life of mice. Against current thinking we show in vivo that TRPM6 is not required for embryonic development per se, but primarily operates in placenta and intestine to regulate Mg2+ levels by transcellular transport, while TRPM6 function in the kidney – commonly thought to be essential – is expendable for organismal Mg2+ balance. We demonstrate that ablation of TRPM6 in adult mice leads to reduced lifespan, growth defects and profoundly impaired health of mutant mice due to defective energy metabolism. We also show that dietary Mg2+ supplementation is not only sufficient to prevent all Trpm6 null pathologies, but Mg2+ is the only mineral known so far able to extend the lifespan of wildtype mice.
It has been reported that constitutive Trpm6 inactivation leads to embryonic lethality, resulting in the generally accepted tenet that Trpm6 is required for the development of the embryo proper (Walder et al., 2009). On the contrary, we provide genetic evidence that the embryonic mortality of Trpm6-deficient mice is caused by the loss of TRPM6 activity in placental SynT-I and yolk sac endoderm cells, and that Trpm6-deficient embryos are depleted of Mg2+. Altogether, we postulate that Trpm6 controls the maternal Mg2+ supply to the fetus, and that growth failure and death are secondary phenotypes induced by Mg2+ deprivation. Differences in the role of TRPM6 for embryonic survival of humans and mice may be attributable to different morphologies of the placental exchange interfaces, fetal growth rate, litter size and dietary preferences (Simmons and Cross, 2005). However, at present we cannot exclude that loss-of-function mutations in TRPM6 might be associated with prenatal mortality in humans as well. Pioneering positional cloning studies (Schlingmann et al., 2002; Walder et al., 2002) and follow-up case reports (Schlingmann et al., 2005; Konrad and Schlingmann, 2014) focused on hospitalized human infants selected by the criterion of deleterious Mg2+ deficiency. Hence, additional TRPM6 phenotypes, such as infertility or embryonic death, might have been overlooked. Accordingly, it has recently been shown that single nucleotide polymorphisms in human TRPM6 are associated with a neural tube closure defect, i.e. meningomyelocele (Saraç et al., 2016). Of note, dietary Mg2+ supplementation is common practice of pregnant women (Durlach et al., 2004). Our work provides first mechanistic insight as to how this essential mineral is delivered to the fetus.
There is growing evidence to suggest that Mg2+ deprivation is involved in the development of metabolic, immune, cardiovascular and neurological disorders (de Baaij et al., 2015). However, due to the lack of adequate mammalian genetic models, it is still unclear whether impaired Mg2+ homeostasis can be regarded as the cause or the consequence of the latter pathophysiological processes. Therefore, we studied the impact of TRPM6 deletion on postnatal mice. Trpm6-deficient mice displayed shorter lifespans, failure to thrive, low physical activity, kyphosis, lung emphysema, sarcopenia and degeneration of lymphoid organs. In addition, Trpm6-deficient animals developed signs of catabolic metabolism such as lipodystrophy, increased insulin sensitivity and hypothermia, and showed suppression of the somatotropic axis accompanied by induction of xenobiotic detoxification gene networks in the liver. Altogether, we noted that the complex phenotype of Trpm6-deficient mice mirrors many phenotypic hallmarks of mutant mouse strains that are generally considered genetic models of ‘accelerated aging’ in the scientific literature (López-Otín et al., 2013). Furthermore, our unbiased screen for metabolic pathways dysregulated in Trpm6-deficient mice revealed an error in energy metabolism reminiscent of humans with mutations in the gene coding for mitochondrial carnitine palmitoyltransferase II, the most common inherited disorder of lipid metabolism in adult humans (Bonnefont et al., 2004; Gempel et al., 2002). Interestingly, an early biochemical study revealed that Mg2+ and MgATP could regulate activity of mitochondrial carnitine acyltransferase activity (Saggerson, 1982). Accordingly, in proof-of-concept experiments, we demonstrated that Mg2+ is specifically required for the utilization of acyl carnitines (AC) as an energy source in liver mitochondria. Hence, these results suggest that insufficient mitochondrial utilization of AC represents a plausible mechanism contributing to the pathologies developed by Trpm6-deficient mice. The exact role of Mg2+ in AC metabolism as well as the molecular pathophysiology of carnitine palmitoyltransferase II deficiency is still not completely understood and, being beyond of the scope of the current manuscript, has to be addressed in future studies.
Notably, if Trpm6-deficient mice were fed with a high Mg2+ diet, they were viable and displayed normal physical activity, morphology of internal organs and tissue levels of AC, indicating that the phenotypes in Trpm6-deficient mice were triggered by Mg2+ deficiency. Therefore, we studied in more detail how mutant mice develop organismal Mg2+ deprivation. According to current thinking, the active transport of Mg2+ in the kidney, in particular in DCT, determines the final urinary Mg2+ concentration and is central to whole-body Mg2+ balance (de Baaij et al., 2015). Contrary to this model, we demonstrate that global inactivation of Trpm6 leads to Mg2+ deficiency due to a defect in intestinal Mg2+ uptake. To interrogate the renal role of TRPM6, we conditionally inactivated Trpm6 in the kidney and, surprisingly, did not observe any changes in serum Mg2+ levels. In contrast, intestine-specific disruption of Trpm6 resulted in hypomagnesemia indicating that wildtype kidneys were not able to counterbalance the ablation of TRPM6 in the intestine. Taken together, our findings lend strong support to the new concept that Trpm6-dependent Mg2+ uptake by the colon plays an indispensable role for systemic Mg2+ balance in mice. However, we assume that renal TRPM6 activity may have an important role under conditions of insufficient dietary Mg2+ intake, for instance, during prolonged fasting periods.
Currently there are two suggested mechanistic models of hypomagnesemia in humans carrying mutations in TRPM6. A pioneering study of patients with congenital hypomagnesemia anticipated that Mg2+ malabsorption in the intestine plays a key role in organismal Mg2+ deprivation (Friedman et al., 1967; Milla et al., 1979). More recently, it was reported that in affected humans renal Mg2+ loss plays a key role (Schlingmann et al., 2002; Walder et al., 2002). The results obtained with Trpm6-deficient mice are well compatible with the former model. However, we cannot exclude physiological differences between humans and mice, an important issue to address in future studies. In conclusion, our findings support the idea that TRPM6 is a central gatekeeper of organismal Mg2+ balance in mammals and that this role cannot be compensated for by any other Mg2+ channel and transporter.
What is the molecular mechanism underlying insufficient Mg2+ intake in Trpm6-deficient mice? TRPM6 and its close homolog TRPM7 have been invoked as molecular correlates of cation currents responsible for Mg2+ entry into cells. In order to circumvent the limitations and pitfalls imposed by overexpression of recombinant proteins, we employed TS cells to compare the roles of native TRPM6 and TRPM7. We found that stem cells express both TRPM6 and TRPM7, thus mimicking the in vivo situation. Inactivation of Trpm6 did not affect the self-renewal of TS cells. In contrast, Trpm7-deficient cells were not able to proliferate unless the cell culture medium was supplemented with additional Mg2+, supporting the idea that TRPM7 plays a non-redundant role in cellular Mg2+ uptake (Schmitz et al., 2003; Chubanov et al., 2004; Ryazanova et al., 2010). We further showed that wildtype TS cells exhibited [Mg2+]i- and [MgATP]i-sensitive divalent cation currents, and that inactivation of Trpm6 reduced current amplitudes. In contrast, deletion of TRPM7 caused complete ablation of these currents. Remarkably, ion currents in Trpm6 deficient TS cells still expressing TRPM7 were substantially more sensitive to intracellular MgATP when compared to TRPM6/M7 co-expressing cells. Thus, in a native environment the presence of TRPM6 reduces the sensitivity of TRPM6/M7-like currents to inhibition by intracellular MgATP. It has recently been reported that the TRPM6/M7 heteromer is completely insensitive to MgATP after heterologous expression in HEK 293 cells (Zhang et al., 2014). Thus, native TRPM6/M7 currents do not fully recapitulate the latter findings obtained in a heterologous expression system and further studies are required to clarify these discrepancies. Nevertheless, our results are concordant with our model (Chubanov et al., 2004) that native TRPM6 functions primarily as a subunit of heteromeric TRPM6/M7 complexes by increasing current amplitudes and relieving TRPM7 from inhibition by [MgATP]i (Figure 6G). Such facilitated Mg2+ entry is most probably not required for any cell autonomous function, but is necessary for epithelial Mg2+ transport and the maintenance of serum Mg2+ levels.
Inadequate nutritional Mg2+ intake is commonplace in Western societies (in up to 68% of the US population [King et al., 2005]). In addition, a growing percentage of the population is exposed to drug-induced forms of hypomagnesemia (de Baaij et al., 2015). Consequently, we asked whether wildtype mice would benefit from extra Mg2+ supplementation. Our proof-of-concept experiments suggest that life-long Mg2+ supplementation may extend the lifespan of mice. This idea is concordant with the recent observation that Mg2+acting alone or in conjunction with dietary calorie restriction counteracts lifespan-shortening effects of RNA-DNA hybrid (R loop) accumulation in yeasts and human HeLa cells (Abraham et al., 2016). Hence, large-scale investigations of nutritional Mg2+ adjustments and their impact on health- and lifespan of different species should be enlightening in this regard.
Experiments involving animals were done in accordance with the EU Animal Welfare Act and were approved by the local councils on animal care (permit No 55.2-1-54-2532-134-13 from the Government of Oberbayern, Germany, and permit No 2347-15-2014 from the State Ministry of Brandenburg, Germany).
A mouse line carrying a gene-trap mutation in Trpm6 (129S5/SvEvBrd-C57BL/6) (Woudenberg-Vrenken et al., 2011) was obtained from the Texas Institute for Genomic Medicine (stock No. TG0020; RRID:IMSR_TAC:tf0834). In the mutant allele (Trpm6βgeo), exons 2 and 3 of Trpm6 were replaced by a βgeo reporter sequence (βgeo). The βgeo cassette includes a splice acceptor site sequence (SP), a bacterial β-galactosidase marker (LacZ), an internal ribosome entry site (IRES) and a neomycin resistance sequence (NeoR). Consequently, the Trpm6βneo allele drives the expression of an aberrant transcript encoding only exon 1 of Trpm6 spliced to βgeo. In order to obtain the Trpm6βgeo allele in a C57BL/6J genetic background, Trpm6βgeo/+ 129S5/SvEvBrd-C57BL/6 mice were backcrossed with C57BL/6J mice for six generations. Mice were genotyped using PCR analysis of genomic DNA isolated from tail fragments. DNA was extracted and purified using the GenElute mammalian genomic DNA isolation kit (Sigma-Aldrich). DNA samples were examined by PCR using a set of allele-specific oligonucleotides (Metabion) and REDTaq DNA polymerase (Sigma-Aldrich). Trpm6βgeo allele was analyzed using primers Trpm6βgeo-Forward 5’-GCGTTGGCTACCCGTGAT-3’ and Trpm6βgeo-Reverse 5’-CTGATAAGGAAGGCTGCTCTAAG-3’ with PCR settings: 94°C 3', 94°C 30'', 55°C 30'', 72°C 1', 40 cycles, 72°C 5'. The amplified PCR product (367 bp) was visualised by standard agarose gel electrophoresis and confirmed by sequencing.
A mouse line (C57BL/6) carrying a conditional mutation in Trpm6 was generated by Taconic Artemis (Köln, Germany). Mouse genomic fragments corresponding to the targeted Trpm6 segment were obtained from the C57BL/6J RPCIB-731 BAC library. The mutant locus contained two intronic LoxP sites flanking exon 17 of Trpm6. In addition, the targeting construct contained thymidine kinase (TK) and neomycin resistance (NeoR) markers allowing for negative and positive selection of mutant ES clones. NeoR was flanked by additional FRT recombination sites allowing for the subsequent deletion of NeoR sequences using Flp recombinase. BAC fragments, recombination sites, NeoR and TK were assembled in the targeting vector using an ET-cloning approach. The final targeting construct was confirmed by sequencing. C57BL/6N Tac embryonic stem (ES) cells were grown on mitotically inactive mouse embryonic fibroblasts (MEF) in DMEM (high glucose) containing 20% FBS (PAN) and 1200 µg/mL leukemia inhibitory factor (Millipore). The authentication of ES cells was performed by Taconic Artemis and no further authentication was performed by the authors. 107ES cells were electroporated (Biorad Gene Pulser) with 30 μg linearized targeting vector. G418 (200 μg/mL) selection was started on day 2 and counter-selection with gancyclovir (2 μM) was started on day 5 after electroporation. ES clones obtained were examined for homologous recombination and single integration by Southern blotting (SB) using two probes located in 3’ and 5’ arms of the targeting construct and one additional probe located in NeoR. Sequences of the targeting vector, probes and SB images are available upon request. Mutant ES cells (clone 3339-AG-1) were injected into blastocysts isolated from uteri of 3.5 days post coitum (dpc) BALB/c females. The injected blastocysts were transferred to uteri of 2.5 dpc pseudopregnant females. Chimerism of the offspring obtained (G0) was evaluated by coat colour contribution (white/black). Highly chimeric G0 males were crossed with C57BL/6 females carrying a ubiquitously expressed Flp transgene to delete the NeoR cassette from the mutant locus. Germline transmission in G1 offspring was identified by the presence of black coat colour followed by PCR analysis of genomic DNA isolated from tail fragments.
Deletion of exon 17 using Cre recombinase results in a frame-shift in the Trpm6 transcript. The efficiency of Cre-mediated deletion of the floxed exon 17 (referred to as Trpm6Δ17 allele) was examined using females ubiquitously expressing the Sox2-Cre transgene. The maternally inherited Sox2-Cre transgene allows for efficient recombination of LoxP-flanked genomic sequences in the single-cell embryo (Hayashi et al., 2003). This approach was employed to produce Trpm6Δ17/+ individuals (Table 1). Mice were genotyped using PCR analysis of genomic DNA isolated from the tail fragments as described above for Trpm6βgeo/+ mice. Trpm6+ allele was analyzed using primers Trpm6+-Forward 5’-AGAGACGTGCAGTGTAGGACAGAG-3’ and Trpm6+-Reverse 5’-ACGGCACACAGAAAACACCAG-3’ with PCR settings: 94°C 3', 94°C 30'', 64°C 30'', 72°C 1', 40 cycles, 72°C 5' (PCR product 549 bp). Trpm6fl allele was studied using primers Trpm6fl-Forward 5’-GCAAATACAAGCAACACCTCC-3’ and Trpm6fl-Reverse 5’-GAAGTTCCTATTCCGAAGTTCC-3’ with PCR settings: 94°C 3', 94°C 30'', 53°C 30'', 72°C 1', 40 cycles, 72°C 5' (PCR product 368 bp). Trpm6Δ17 mutation was determined using primers Trpm6Δ17-Forward 5’-TGTCTTCCATGTTGCTACGA-3’ and Trpm6Δ17-Reverse 5’-CTTCCGGTCCACAGTTCAT-3’ with PCR settings: 94°C 3', 94°C 30'', 53°C 30'', 72°C 1', 40 cycles, 72°C 5' (PCR product 362 bp). Amplified PCR products were confirmed by sequencing.
Transgenic mice expressing Cre recombinase under the control of the mouse Sox2 promoter (Sox2-Cre mice, C57BL/6J; RRID:IMSR_HAR:3359) (Hayashi et al., 2003), Kidney-specific/Cadherin16 promoter (Ksp-Cre mice, C57BL/6J; RRID:MGI:4452131) (Shao et al., 2002) and Villin1 promoter (Villin1-Cre mice, C57BL/6J; RRID:MGI:3581405) (Madison et al., 2002) were obtained from the Jackson Laboratory (Sacramento, CA, USA, stock No. 008454, 012237, and 004586 respectively). To conditionally inactivate Trpm6, Sox2-Cre, Ksp-Cre and Villin1-Cre mice were crossed with Trpm6Δ17/+ mice to produce Trpm6Δ17/+;Sox2-Cre, Trpm6Δ17/+;Ksp-Cre, and Trpm6Δ17/+;Villin1-Cre males. Next, these males were bred with Trpm6fl/fl females to generate offspring with a global, kidney and intestine-restricted disruption of Trpm6 as outlined in Table 1. Inheritance of Sox2-Cre, Ksp-Cre and Villin1-Cre transgenes was determined by PCR analysis of tail DNA at conditions described previously (Madison et al., 2002; Shao et al., 2002; Hayashi et al., 2003).
A mouse line carrying a conditional mutation in Trpm7 (Trpm7fl/flmice, 129 S6/SvEvTac; RRID:IMSR_JAX:018784) was kindly provided by David Clapham, Harvard Medical School, Boston, USA. The mutant locus contains two intronic LoxP sites flanking exon 17 of the Trpm7 gene. Deletion of exon 17 results in a frame-shift mutation (Trpm7Δ17 allele) and subsequent disruption of TRPM7. To produce Trpm7Δ17/+ mice, Trpm7fl/fl females were crossed with Sox2-Cre males and the resulting offspring were genotyped by PCR as described previously (Jin et al., 2008). The generated Trpm7Δ17/+ strain was maintained by intercross of Trpm7Δ17/+ parents.
Mice were kept in individually ventilated polycarbonate cages (IVC System, Tecniplast, Germany). Cages were changed weekly and were on a 12 hr light/dark cycle with artificial lighting. Temperature and relative humidity were 22 ± 1°C and 50 ± 5%, respectively. Breeding animals were maintained on a multigrain chow Ssniff M-Z (Ssniff GmbH, Germany) and drinking water containing 20.1 mg/l Mg2+ and 81.9 mg/l Ca2+ (ad libitum). Litters were weaned at three weeks of age, genotyped and desired littermates were housed in cages as described above except that a ‘maintenance’ chow Sniff R/M-H (Ssniff GmbH, Germany) was used. Both Ssniff M-Z and Ssniff R/M-H chows contained 0.22% Mg2+ and 1.0% Ca2+. In dietary Mg2+ supplementation experiments, 4 week-old Trpm6-deficient and control mice were fed Ssniff R/M-H chow containing 0.75% Mg2+ (Ssniff GmbH, Germany).
Mice were weighed, killed and blood samples were collected by a cardiac puncture technique. Blood samples were incubated 30 min at RT, clots were removed by centrifugation (Heraeus Pico 17, 3500 rpm for 30 min at RT) and the resulting serum samples were stored at −80°C. Internal organs were removed, flash frozen in liquid nitrogen and stored at −80°C. Right tibias were dissected, cleaned from muscle tissues, dried overnight at 70°C and kept at RT. To determine urinary and fecal excretion rates of main minerals, mice were maintained for 24 hr in individual metabolic cages (Acme Metal Products, USA) under housing conditions as described above and supplied ad libitum with drinking water and chow. After 24 hr, the urine and feces produced were collected. Urine samples were stored at −80°C. Feces specimen were dried overnight at 70°C and stored at −20°C.
Food and water intake were recorded with an automated Drinking and Feeding Monitor system (TSE Systems, Germany) with food baskets connected to weight sensors. An adaptation period of 2 days was followed by 3 days of data collection. Cumulative food intake and locomotor activity (based on infrared beams, InfraMot-Activity System, TSE Systems, Germany) were recorded. The core body temperature was measured using a rectal probe as reported previously (Braun et al., 2009). In vivo analysis of body lean mass was performed in conscious, restrained mice by nuclear magnetic resonance (EchoMRITM-100H; EchoMRI LLC, USA) weekly and before metabolic measurements. The energy content in feces was determined by bomb calorimetry (IKA C5003; IKA Werke, Staufen, Germany).
Assessment of atherosclerosis lesions was performed as described previously (van der Vorst et al., 2015).
Serum concentrations of IGF1 were analysed by a Mouse/Rat IGF-I ELISA kit (R and D-Systems). MUPs content as assessed by SDS-PAGE gel electrophoresis of 1 µl urine samples from individual mice. ß-Hydroxybutyrate was measured by a commercially available assay kit (Sigma-Aldrich). For a glucose-tolerance test, mice were fasted for 6 hr, followed by an oral gavage of glucose (2 mg/g body weight). Blood samples were collected via the tail vein and blood glucose levels were measured using a contour glucometer (Bayer, Germany). Plasma levels of insulin were measured using a commercially available ELISA kit (Alpco).
Tissues were fixed overnight at 4°C in 4% paraformaldehyde (PFA), dehydrated through a series of ethanol washes, cleared in three changes of xylene and embedded in paraffin. Tissue sections (5 μm) were cut by RM2125 RTS microtome (Leica Microsystems, Germany), mounted on Superfrost Plus slides (Menzel-Gläser), and dried at 70°C for 1 hr. Tissue sections were dewaxed in xylene, rehydrated through a series of ethanol washes to deionized water. Slides were incubated in Mayers hematoxylin solution (Carl Roth) for 10 min at RT followed by incubation in 0.5% Eosin Y solution (Carl Roth) for 13 min at RT. The colour reaction was stopped by deionized water, slides were dehydrated in ethanol, cleared in xylene and mounted using mounting medium (Carl Roth). Slides were examined using an Olympus CX41 microscope and Cell Imaging software (Olympus, Germany).
Polyclonal TRPM6-specific antibodies were raised by immunization of rabbits with H2N-CERDKNRSSLEDHTRL-COOH peptide coupled via the N-terminus to keyhole limpet hemocyanin (KLH) and purified by peptide affinity chromatography (Eurogentec, Belgium). Whole kidneys were dissected from eight week-old mice and embedded in Jung tissue freezing medium (Leica Microsystems, Germany). 10 μm cryosections were produced by a CM 3050S cryotom (Leica Microsystems, Germany), mounted on Superfrost Plus slides (Menzel-Gläser), air-dried for 20 min and fixed in 2% (w/v) paraformaldehyde in PBS (pH 7.4) for 20 min at RT. After washing in PBS (2×10 min), sections were blocked with 5% goat serum/0.5% Triton X100 (Sigma-Aldrich) in PBS for 2 hr at RT. The rabbit anti-TRPM6 antibody (1 μg/ml in 5% goat serum/0.5% Triton X100/PBS) was applied overnight at 4°C. Afterwards sections were washed in PBS (3×10 min, RT) and a goat anti-rabbit antibody conjugated with Alexa 488 (Life Technologies, Darmstadt, Germany; 1 μg/ml in 5% goat serum/PBS) was applied for 1 hr at RT. After washing in PBS (3×10 min, RT), sections were embedded in Dako Mounting Fluid (Dako Cytomation). Differential interference contrast and confocal images were obtained with a confocal laser scanning microscope LSM 540 META (Carl Zeiss, Germany). We used a Plan-Apochromat x63/1.4 oil objective, the 488 nm excitation wavelength of an argon laser, and a 505–570 nm band-pass filter. Acquired DIC and confocal images were analysed using the LSM 540 META software (Carl Zeiss, Germany).
cDNA templates for the production of cRNA Trpm6 probes were produced by PCR with the following 2 sets of primers: Probe-1-Forward: 5’-aattaaccctcactaaagggGAGAGGAGGCCACAGTCAAG-3’; Probe-1-Reverse: 5’-taatacgactcactatagggGCTCAAAGACGATGTCACGA-3’; Probe-2-Forward: 5’-aattaaccctcactaaagggCCTGTCAAAGAAGAAGAGGAA-3’; Probe-2-Reverse: 5’-taatacgactcactatagggAGAAAAGACTTCACAATG-3’. Primers contained T7 (reverse primer lower case) or T3 (forward primer lower case) RNA polymerase sites. PCR products were gel purified (Qiagen Gel Extraction Kit) and sequence verified (ARGF). Digoxigenin (DIG) labelled cRNA probes were synthesized according the manufacturer’s instructions (Roche, 10x DIG RNA labelling kit). Both probe sets produced similar results on n = 5 placentas at each gestational time point examined, and n = 3 WT kidney or intestinal samples. Gcm1 and SynA riboprobes were described previously (Dawson et al., 2012). Preparation of tissue sections and ISH procedures were performed as previously described (Dawson et al., 2012). Slides were imaged by an Aperio slide scanner and analysed using ImageScope software.
Microarray data were deposited in NCBI Gene Expression Omnibus (GEO) (GSE70457). Liver tissues were collected from 12–13 week-old Trpm6-deficient (Trpm6Δ17/Δ17;Sox2-Cre, n = 3) and control (Trpm6+/fl, n = 4) male littermates, snap-frozen in liquid nitrogen and stored at −80°C. Total RNA was extracted using the GenElute mammalian total RNA purification kit (Sigma-Aldrich). Whole genome profiling was performed using a GeneChip Mouse Gene 1.0 ST Array (Affymetrix) at Source Bioscience (Berlin, Germany). Biotinylated single-stranded DNA was prepared according to the standard Affymetrix protocol (Whole Transcript Expression arrays) from 100 ng total RNA using the WT terminal labelling kit. 2.5 µg of fragmented and labelled ssDNA were hybridized for 16–18 hr at 45°C. GeneChips were washed and stained in an Affymetrix Fluidics Station 450. GeneChips were scanned using the Affymetrix GeneChip Scanner 3000. Processing of the array data, including quality assessment, background correction, normalization and summarization was performed with the Affymetrix Expression Console (version 1.4.0). All statistical analyses were carried out with the statistical computing environment R (version 3.1.2, www.R-project.org). Differential expression analysis was performed with the R package limma (version 3.22.4) (Ritchie et al., 2015). p-values were adjusted for multiple testing with the Benjamini-Hochberg method for controlling the false discovery rate (FDR). A heatmap was generated for a group of 46 transcripts differentially expressed at a level of FDR p≤0.1. Analysis of the affected pathways and causal transcriptional regulators was performed by Ingenuity pathway analysis (IPA) environment (www.ingenuity.com, RRID:SCR_008653) using a set of 2443 transcripts changed at p≤0.05 confidence level (t-test).
Serum, liver and gastrocnemius muscle samples were collected from 8–10 week-old control (Trpm6+/fl, n = 8) and Trpm6-deficient (Trpm6Δ17/Δ17;Sox2-Cre, n = 6) male littermates, flash frozen in liquid nitrogen and stored at −80°C. Metabolomic analyses were performed at Biocrates Life Sciences AG (Innsbruck, Austria). Measurements comprised the quantification of 237 metabolites including 41 amino acids/biogenic amines, 40 acylcarnitines (AC), 22 bile acids (BA), 14 lysophosphatidylcholines (LysPC), 77 phosphatidylcholines (PC), 15 sphingomyelins (SM), 17 eicosanoids/prostaglandins and 11 energy metabolism intermediates as outlined in Supplementary file 3. To extract metabolites, tissue samples were treated with corresponding extraction buffers and incubated in a chilled ultrasonic bath for 5 min. Afterwards samples were centrifuged and the supernatant was used for analysis. FIA- and LC-MS/MS measurement techniques were applied as described in detail previously (Pena et al., 2014). All statistical analyses have been applied by using the statistical computing environment R (www.r-project.org). Metabolite measurements containing more than 75% missing values or more than 75% of values below the limit of detection (LOD) across all samples per matrix were removed from analysis. Measured concentrations of metabolites were log2-transformed for moderated statistical tests. Measurements were scaled to µ = 0 mean and unit standard deviation for each biological matrix separately for heatmap visualization. Changes in average metabolite levels between control and mutant individuals were tested using a linear model framework implemented in the R package Limma (Smyth, 2004). Resulting P-values for moderated t-test were corrected for multiple testing by Benjamini and Hochberg approach. A p-value threshold of 0.05 was considered as significant. A Heatmap diagram for metabolites with significant changes was calculated with the R-package Heatmap using ward clustering and Euclidean distance measure and the R-package VennDiagram was applied to calculate a Venn diagram of significantly changed metabolites across sample matrices.
Mouse liver mitochondria were isolated by differential centrifugation from freshly prepared homogenates as previously described (Springer et al., 2002; Schulz et al., 2015). Liver mitochondria were further purified by Percoll density gradient centrifugation (Schulz et al., 2013). Isolated mitochondria were subjected to quantification by the Bradford assay and kept on ice until use. Assessment of the mitochondrial membrane potential Δψm (MMP) was followed by Rh123 fluorescence quenching (Ex. 485 nm, Em. 528 nm) in a 96-well plate reader (BioTek) and quantitatively evaluated by curve analysis as previously described (Schulz et al., 2013) (set threshold slopes were ≥0.67 for start points and ≤0.67 for end points, respectively). In order to exclude fluctuations at measurement start, slope calculations were started after 6 min measurement time with slope values ≤ 1.5. A kit-based assay (ATP Bioluminescence Assay Kit, Roche) was used to analyze the ATP content from cleared lysates after 30 min mitochondrial ATP synthesis at RT, initiated by the addition of 160 µM ADP and stopped at 95°C for 5 min. For both analyses assay buffer composition was 0.2 M sucrose, 10 mM MOPS-Tris, 1 mM Pi and 10 µM EGTA. Respiratory substrates were either succinate (25 mM)/rotenone (2 µM), or DL-octanoylcarnitine (10 µM)/malate (12.5 mM), or DL-palmitoylcarnitine (10 µM)/malate (12.5 mM). Buffers and solutions were essentially Mg²+-free, as determined by ICP-OES (Ciros Vision, SPECTRO Analytical Instruments GmbH) after wet ashing with 65% nitric acid (Zischka et al., 2011). EDTA, Mg2+, Ca2+, or Zn2+ was added at the concentrations indicated in the respective figures.
TS cells were isolated as described (Tanaka et al., 1998), with several modifications (Erlebacher et al., 2004; Natale et al., 2009). 3.5 days post-coitum blastocysts were isolated from Trpm6βgeo/+ parents. Individual blastocysts were incubated in a humidified cell culture incubator (Heraeus, Thermo Fisher Scientific) at 37% and 5% CO2 for 3 days in 12 well plates (Sarstedt) containing 8×104/well irradiated mouse embryonic fibroblasts (MEFs) (Millipore) in RPMI 1640 medium (Life Technologies) supplemented with 20% fetal bovine serum (ES type, Life Technologies), 1 mM sodium pyruvate (cell culture type, Sigma-Aldrich), 100 µM β-mercaptoethanol (Sigma-Aldrich), 50 µg/ml streptomycin and 50 U/ml penicillin (all from Life Technologies), 1.0 µg/ml heparin (cell culture type, Sigma-Aldrich), 25 ng/ml human recombinant FGF4 (R and D systems), 5 ng/ml human recombinant TGF-β1 (R and D systems), 10 ng/ml recombinant activin A (R and D systems) and an additional 10 mM MgCl2. The attached embryos were disaggregated by 0.05% trypsin-EDTA (Life Technologies) and derived cells were further co-cultured with MEFs as described above. The obtained TS cells were propagated and adapted to MEF-free conditions without additional Mg2+ in the culture medium. The authentication of TS cells was based on expression of a trophoblast stem cell marker Esrrb, characteristic morphological appearance and ability to proliferate only in the presence of FGF4 and TGF-β1 (Tanaka et al., 1998; Simmons and Cross, 2005). TS cells were tested negative for mycoplasma contamination using QuickTest kit (Biotool).
Genotypes of TS cells were determined by PCR analysis of genomic DNA using conditions described above for Trpm6βgeo/+ mice. TS cells were further examined by RT-PCR. Total RNA was extracted from TS cell pellets using the GenElute mammalian total RNA purification kit (Sigma-Aldrich). First strand cDNA synthesis was performed by RevertAid H minus reverse transcriptase (Thermo Scientific). PCR was performed using REDTaq DNA polymerase (Sigma-Aldrich) with two primer sets: Trpm6a-Forward 5’-GCTGCCAAATCTGCCACAAT-3’ and Trpm6a-Reverse 5’-TGCCCACAGTCCCATCATCACA-3’ or Trpm6b-Forward 5’-CCAGCTCAAAAGACCCTCACAGATGC-3’ and Trpm6b-Reverse 5’-CACACCACATCTTTTCCGACCAG-3’. The following PCR conditions were used: 94°C 3', 94°C 30'', 56°C 30'', 72°C 1', 35 cycles, 72°C 5'. Amplified PCR products were 651 bp or 586 bp, respectively. Self-renewal of Trpm6βgeo/βgeo TS cells was assessed by determination of DNA content as described previously (Tanaka et al., 1998). Briefly, TS cells were cultured for 3 days, dissociated by trypsin-EDTA and fixed with 40% ice-cold ethanol. Fixed TS cells were incubated in propidium iodide (PI) staining solution (50 μg/ml PI, 0.2 mg/ml RNaseA in PBS, all from Sigma-Aldrich) for 30 min at RT. Stained cells were dissolved in PBS and examined using BD FACSCalibur (BD Biosciences, Germany) and FlowJo software (www.flowjo.com).
Trpm7-deficient and corresponding control TS cells were derived as described above using Trpm7Δ17/+ mice. The obtained Trpm7+/+ and Trpm7Δ17/Δ17 TS cells were propagated and adapted to MEF-free conditions in cell culture medium supplemented with 10 mM MgCl2. Genotypes of TS cells were determined by PCR analysis of genomic DNA using conditions described for Trpm7Δ17/+ mice. The lack of Trpm7 transcripts in Trpm7Δ17/Δ17 TS cells was verified by RT-PCR using primers Trpm7-Forward 5’-AGTAATTCAACCTGCCTCAA-3’ and Trpm7-Reverse 5’-ATGGGTATCTCTTCTGTTATGTT-3’ and the following PCR conditions: 94°C 3', 94°C 30'', 50°C 30'', 72°C 1', 35 cycles, 72°C 5'. The amplified PCR product was 287 bp.
To study growth rates, TS cells of each genotype were seeded in 6-well plates (1×105 cells/well) in cell culture medium containing 10 mM MgCl2. After 24 hr (day 1), the culture medium was replaced with fresh medium either with or without 10 mM MgCl2 and the cells were further cultured for additional 3 days. The cell density was determined at 24 hr intervals using a Neubauer chamber (Marienfeld Superior). To calculate growth rates, the cell number at day 1 was designated as 100%. The experiment was repeated three times and a Student’s t-test was applied to compare the growth rates of control versus mutant cells.
Whole-cell currents were measured using an EPC10 patch-clamp amplifier and PatchMaster software (Harvard Bioscience, Germany). Voltages were corrected for a liquid junction potential of 10 mV. Currents were elicited by a ramp protocol from −100 mV to +100 mV over 50 ms acquired at 0.5 Hz and a holding potential of 0 mV. Inward and outward current amplitudes were extracted at −80 mV and +80 mV and were normalized to cell size as pA/pF. Capacitance was measured using the automated capacitance cancellation function of EPC10. Patch pipettes were made of borosilicate glass (Science Products) and had resistances of 2–3.5 MΩ. The standard extracellular solution contained (in mM): 140 NaCl, 3 CaCl2, 2.8 KCl, 10 HEPES, and 11 glucose (all from Sigma-Aldrich). A divalent-free (DVF) extracellular solution contained (in mM): 140 NaCl, 2.8 KCl, 10 HEPES, 11 glucose and 5 EDTA. TRPM6/M7-like currents were induced by Mg2+-free intracellular solution, containing (in mM): 140 Cs-glutamate, 8 NaCl, 10 EGTA, 5 EDTA and 10 HEPES. All solutions were adjusted to pH 7.2 using a FE20 pH-meter (Mettler Toledo, Germany). The osmolality of all solutions was adjusted to 290 mOsm using Vapro 5520 osmometer (Wescor Inc., USA). Data were compared by an unpaired Student’s t-test.
For Mg2+ ([Mg2+]i) and MgATP ([MgATP]i) dose responses, the intracellular pipette solution contained (in mM): 120 Cs-glutamate, 8 NaCl, 10 HEPES, 2.7 EDTA and various amounts of MgCl2 or MgATP (Sigma-Aldrich). The solutions were adjusted to pH 7.2 and 290 mOsm. Concentrations of MgATP and free Mg2+ were calculated using WebMaxC (maxchelator.stanford.edu). To determine IC50 values, datasets were fitted using a nonlinear (least-squares) regression analysis (GraphPad Prism 6.0 software) and the following equation:
with E being the effect/current at a given concentration C of inhibitor, Emin the minimal effect/current, Emax the maximally achievable effect, IC50 the half-maximal concentration and h the Hill slope factor. Statistical analysis of dose-response curves and IC50 values was performed using the extra sum-of-squares F test with the threshold P value 0.05 (GraphPad Prism 6.0).
Wildtype parental cells (clone C631) and TRPM7-deficient (clone 10940–04) HAP1 cells were acquired from Horizon Genomics (Vienna, Austria). The authentication of HAP1 cells was performed by Horizon Genomics (Essletzbichler et al., 2014) and no further authentication was performed by the authors. A CRISPR/Cas9 approach was used to introduce a 17 bp (GTGACCATTTTAATCAG) deletion in exon 4 of the human TRPM7 gene resulting in a frame-shift mutation. Genotypes of HAP1 cells were confirmed by PCR amplification of genomic DNA using primers hTRPM7-Forward 5’-TATTTGTATGCACCTTTGTA-3’ and hTRPM7-Reverse 5’-TGTTTTAATCTCACCTTTTT-3’ with PCR parameters: 94°C 3', 94°C 30'', 50°C 30'', 72°C 1', 40 cycles, 72°C 5'. PCR products (364 bp and 347 bp in wild type and mutant clones, respectively) were confirmed by sequencing. HAP1 cells were tested negative for mycoplasma contamination using QuickTest kit (Biotool).
HAP1 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FBS and 100 U/ml penicillin, 100 µg/ml streptomycin and 10 mM MgCl2 (all from Thermo Fisher Scientific). Cells were maintained in a humidified cell culture incubator (Heraeus, Thermo Fisher Scientific) at 37°C and 5% CO2. Western blot analysis was performed as described previously (Nörenberg et al., 2016). Examinations of growth rates and endogenous TRPM7-like currents were conducted as described above for TS cells.
HAP1 cells of each genotype were grown in T175 cm2 flasks (Sarstedt) in 10 mM MgCl2 supplemented cell culture medium as described above. At ~50% confluence the medium was replaced with fresh medium without 10 mM MgCl2 and the cells were cultured for additional 24 hr. Next, cells were washed with PBS, disaggregated by trypsinization and collected in 50 ml plastic tubes (Sarstedt). After centrifugation (3 min, 1000 rpm), the cell pellet was resuspended in 1 ml PBS and passed to a fresh 1.5 ml tube. The cell suspension was centrifuged (3 min, 3500 rpm), supernatant was completely removed and the cell pellet was dried overnight at 70°C. The dried cell pellet was analysed by ICP-MS in ALS Scandinavia (Sweden). The experiment was repeated four times.
HAP1 cells of each genotype were seeded in 96-well plates (white wall/clear bottom, type 3610, Costar) at a density of 5×105 cells/well in the 10 mM MgCl2 supplemented cell culture medium (100 µl/well); eight wells per genotype were used. After 24 hr, the cell culture medium was replaced with fresh regular medium (without additional 10 mM Mg2+) and the cells were cultured for additional 24 hr. To determine cell viability, 10 µl of CCK-8 reagent (Cell Counting Kit-8) was added in four wells per genotype. The plates were incubated for 3 hr in the cell culture incubator (Heraeus, Thermo Fisher Scientific) at 37°C and 5% CO2. Next, 96-well plates were incubated at room temperature (RT) for 30 min and 100 µl of CellTiter-Glo2.0 reagent (Promega) was added to the remaining four wells per genotype. After 10 min incubation at RT, either ATP-induced luminescence or CCK-8 absorbance (450 nm) were determined using a plate reader (FLUOstar Omega, BMG Labtech). CCK-8 absorbance was used to normalize ATP-induced bioluminescence. The normalized bioluminescence of wildtype cells was designated 100%. The experiment was repeated six times.
Oxygen consumption of HAP1 cells was assessed by Oxygraph-2k measurements (Oroboros Instruments GmbH, Austria) as described previously (Pesta and Gnaiger, 2012). Briefly, WT and KO HAP1 cells were maintained in standard medium supplemented by 10 mM MgCl2. The Mg2+ supplemented medium was replaced with regular medium (without additional 10 mM Mg2+) and the cells were cultivated for a further 24 hr. Next, oxygen flux from routine respiration of 1.5×106 cells at 37°C and maximal oxygen flux after stepwise CCCP addition (2 µl steps from 1 mM stock solution) were determined. The oxygen flux was baseline-corrected for non-mitochondrial oxygen consuming processes by the addition of 0.5 µM rotenone (complex I inhibitor, Sigma-Aldrich) and 2.5 µM antimycin A (complex III inhibitor, Sigma-Aldrich).
Mice were raised in a specific pathogen-free facility at Jackson Laboratory Sacramento (Sacramento, CA, USA). The long-lived B6C3F1 hybrid strain (Lipman et al., 1999; Turturro et al., 1999) was used. Specifically, F1 females were derived by crossing C57BL/6J females with C3H/HeJ males. The produced B6C3F1 females were weaned at 3 weeks of age, and afterwards kept in individually ventilated polycarbonate cages (Thoren Caging Systems) on multigrain chow 5 K54 (Purina) containing 0.22% Mg2+ and drinking water containing 0.44 mg/l Mg2+. Five mice were housed per cage and cages were changed every two weeks. The housing rooms were with artificial lighting and 12 hr light/dark cycle (6 am to 6 pm). Temperature and relative humidity in animal rooms were 22 ± 4°C and 50 ± 15%, respectively. At 5 months of age, mice were assigned to control, dietary restricted (DR) or supplemented cohorts as outlined in Table 2. For supplementation experiments, corresponding salts (Sigma-Aldrich) were diluted in drinking water that was administered ad libitum throughout the lifespan of all groups (Table 2). To reduce bias, the supplemented and control groups were examined simultaneously and the study was performed as a blinded trial. Mice were inspected daily. Necropsies of randomly selected dead mice revealed that 58 of 88 control females (66%) developed tumours. 8 of 11 females (73%) from the three Mg2+ supplemented groups also had tumours suggesting that high dietary Mg2+ had no substantial effect on tumor rate at death. Kaplan-Meier survival distributions were computed to illustrate survival times. For statistical analysis, we used MATLAB computing environment and programming language (MathWorks, www.de.mathworks.com), in particular its built-in fast convolution function that allows to find the distribution of the sum of independent random variables, given the distributions of individual variables. To enable statistical comparisons between control and dosed groups, the distribution of the mean lifespan of 15 control mice was found as a convolution of the original distribution of lifespans of all control mice. To calculate P-values of the mean lifespan from the dosed groups relative to the control group, we calculated the probability of the average of 15 control mice showing the same or more extreme (away from control mean) lifespan than the experimentally determined mean lifespan of the dosed mice. A similar technique was used to find P-values for pooled Mg and dietary restriction groups relative to controls. The MATLAB code is available from the Dryad Digital Repository (Chubanov and Gudermann, 2016). In addition, the survival data of control mice versus individual treated groups were assessed by log-rank test using GraphPad Prism software. P-values for both approaches are indicated in Figure 7A.
Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspectsMolecular Aspects of Medicine 25:495–520.https://doi.org/10.1016/j.mam.2004.06.004
Magnesium in man: implications for health and diseasePhysiological Reviews 95:1–46.https://doi.org/10.1152/physrev.00012.2014
New data on the importance of gestational mg deficiencyJournal of the American College of Nutrition 17:116–125.https://doi.org/10.1080/07315724.2004.10719411
Maintenance of mouse trophoblast stem cell proliferation by TGF-beta/activinDevelopmental Biology 275:158–169.https://doi.org/10.1016/j.ydbio.2004.07.032
Screening for carnitine palmitoyltransferase II deficiency by tandem mass spectrometryJournal of Inherited Metabolic Disease 25:17–27.https://doi.org/10.1023/A:1015109127986
Endothelial cells and magnesium: implications in atherosclerosisClinical Science 122:397–407.https://doi.org/10.1042/CS20110506
TRPM7 is a molecular substrate of ATP-evoked P2X7-like currents in tumor cellsThe Journal of General Physiology 147:467–483.https://doi.org/10.1085/jgp.201611595
Molecular identification of ancient and modern mammalian magnesium transportersAmerican Journal of Physiology: Cell Physiology 298:C407–429.https://doi.org/10.1152/ajpcell.00124.2009
Determination of low-abundance elements at ultra-trace levels in urine and serum by inductively coupled plasma-sector field mass spectrometryAnalytical and Bioanalytical Chemistry 380:247–257.https://doi.org/10.1007/s00216-004-2742-7
Application of inductively coupled plasma sector field mass spectrometry for elemental analysis of urineJournal of Trace Elements in Medicine and Biology 14:241–247.https://doi.org/10.1016/S0946-672X(01)80010-9
TRPM7 is essential for Mg(2+) homeostasis in mammalsNature Communications 1:109.https://doi.org/10.1038/ncomms1108
Novel TRPM6 mutations in 21 families with primary hypomagnesemia and secondary hypocalcemiaJournal of the American Society of Nephrology 16:3061–3069.https://doi.org/10.1681/ASN.2004110989
A protocol for the parallel isolation of intact mitochondria from rat liver, kidney, heart, and brainMethods in Molecular Biology 1295:75–86.https://doi.org/10.1007/978-1-4939-2550-6_7
Progressive stages of mitochondrial destruction caused by cell toxic bile saltsBiochimica Et Biophysica Acta - Biomembranes 1828:2121–2133.https://doi.org/10.1016/j.bbamem.2013.05.007
Epithelial-specific cre/lox recombination in the developing kidney and genitourinary tractJournal of the American Society of Nephrology 13:1837–1846.https://doi.org/10.1097/01.ASN.0000016444.90348.50
Linear models and empirical bayes methods for assessing differential expression in microarray experimentsStatistical Applications in Genetics and Molecular Biology 3:1–25.https://doi.org/10.2202/1544-6115.1027
Current Protocols in Human GeneticsGene delivery to muscle, Current Protocols in Human Genetics, New York, John Wiley & Sons, 10.1002/0471142905.hg1304s31.
Growth curves and survival characteristics of the animals used in the biomarkers of aging programThe Journals of Gerontology Series A: Biological Sciences and Medical Sciences 54:B492–501.https://doi.org/10.1093/gerona/54.11.B492
TRPM6 forms the Mg2+ influx channel involved in intestinal and renal Mg2+ absorptionJournal of Biological Chemistry 279:19–25.https://doi.org/10.1074/jbc.M311201200
Mice defective in TRPM6 show embryonic mortality and neural tube defectsHuman Molecular Genetics 18:4367–4375.https://doi.org/10.1093/hmg/ddp392
The TRPM6 kinase domain determines the mg·ATP sensitivity of TRPM7/M6 heteromeric ion channelsJournal of Biological Chemistry 289:5217–5227.https://doi.org/10.1074/jbc.M113.512285
Liver mitochondrial membrane crosslinking and destruction in a rat model of wilson diseaseJournal of Clinical Investigation 121:1508–1518.https://doi.org/10.1172/JCI45401
Richard S LewisReviewing Editor; Stanford University School of Medicine, United States
In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.
Thank you for submitting your article "Epithelial magnesium transport by TRPM6 is essential for prenatal development and adult survival" for consideration by eLife. Your article has been favorably evaluated by Richard Aldrich (Senior Editor) and three reviewers, one of whom, Richard S Lewis (Reviewer #1), is a member of our Board of Reviewing Editors. The following individual involved in review of your submission has agreed to reveal their identity: Thomas Voets (Reviewer #2).
The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.
In this paper, Chubanov et al. provide an extensive analysis of the consequences of TRPM6 deficiency in mice, from embryonic development through adulthood. TRPM6 is considered an important regulator of magnesium transport, based on the severe hypomagnesemia observed in patients lacking functional TRPM6 and on Mg-permeable and -regulated ion channel activity in cells heterologously expressing TRPM6. However, the physiological mechanisms and functions of TRPM6 have been difficult to determine because global deletion of TRPM6 in mice causes embryonic death. The authors have used targeted TRPM6 deletions in mice to reveal that embryonic lethality is caused by TRPM6 deficiency in extraembryonic tissues rather than the embryo itself. By targeting the TRPM6 knockout to the embryo, they create a useful model system with which to study the phenotype of TRPM6 postnatally and in adult mice. A detailed characterization of these mice shows that TRPM6-dependent Mg2+ uptake in the colon but not kidney is essential for maintaining systemic Mg2+ homeostasis, and hypomagnesemia in TRPM6-deficient mice impairs growth, metabolism and lifespan, attributable to the impaired utilization of long-chain fatty acids by mitochondria to make ATP. Finally, dietary Mg2+ supplements, in addition to reversing the multiple effects of TRPM6 deletion, also prolong the lifespan of wild type mice, an intriguing observation that awaits replication in other organisms.
All three reviewers agree that the paper is extensive and significantly extends the understanding of TRPM6's role in Mg2+ homeostasis pre- and postnatally, and that the data are generally of high quality and clearly presented. No major new experiments are required, but some straightforward measurements described below would provide direct evidence needed to strengthen two important mechanistic conclusions.
1) The authors suggest that Mg2+ deprivation in TRPM6- deficient mice impairs ATP levels by preventing the use of acylcarnitine as a substrate. The link between Mg2+, AC metabolism and ATP is only shown for isolated mitochondria and nonphysiological concentrations of [Mg2+]. A simple measurement of ATP or mitochondrial potential (using fluorescent dyes) in intact cells from WT vs. KO mice would provide direct support to strengthen this conclusion.
2) The authors propose that TRPM6 regulates Mg2+ transport by reducing the MgATP-dependent inhibition of heteromeric TRPM6/7 channels. First, the evidence for this rests on whether the inhibition curves in Figure 6B and C are significantly different between WT and KO animals; this should be evaluated. In addition, it is not clear that MgATP in intact cells is in the required range that would differentially affect the current in the WT vs. TRPM6 groups (Figure 6B); either the ATP level should be measured, or a suitable reference provided. Finally, another group (Zhang, Yu et al., 2014) reported that TRPM6 expression by itself produced currents, and TRPM6 and M7 coexpression generated currents that were essentially insensitive to MgATP inhibition. This should be discussed in the context of the results shown in Figure 6, where TRPM7 KO completely eliminated current, and wild type and TRPM6 currents are both inhibited by MgATP.https://doi.org/10.7554/eLife.20914.029
1) The authors suggest that Mg2+ deprivation in TRPM6-deficient mice impairs ATP levels by preventing the use of acylcarnitine as a substrate. The link between Mg2+, AC metabolism and ATP is only shown for isolated mitochondria and nonphysiological concentrations of [Mg2+]. A simple measurement of ATP or mitochondrial potential (using fluorescent dyes) in intact cells from WT vs. KO mice would provide direct support to strengthen this conclusion.
Thank you for this important point. The studies with isolated liver mitochondria were conducted because our metabolic profiling experiments suggested that mitochondrial metabolism of acylcarnitines was affected by sustained Mg2+ deficiency in Trpm6-deficient adult mice. Since freshly isolated mitochondria are metabolically active for a very short time only (a few hours), we had to acutely deplete Mg2+ in the mitochondrial matrix using buffers with low Mg2+ concentrations.
When attempting to recapitulate the phenotype of living Trpm6-deficient mice at the cellular levels, several critical issues need to be considered: First, according to our model Trpm6-mediated Mg2+ uptake is not required for cell autonomous functions, implying that Trpm6-deficient cells (like TS cells, Figure 6 and Figure 6—figure supplement 1) will not develop Mg2+ deficiency and abnormal energy metabolism, because TRPM7 is still functional. Second, Trpm6 is not expressed in tissues, which critically rely on mitochondrial energy production, such as skeletal muscle or liver. Furthermore, during the isolation process of primary cells it is virtually impossible to “clamp” intracellular Mg2+ concentrations at exactly the same levels normally occurring in vivo in Trpm6-deficient mice. Accordingly, in vitro experiments with liver or skeletal muscle cells isolated from Trpm6-deficient mice would not truly reflect in vivo conditions of Trpm6-deficient mice, such as prolonged (several weeks) organismal Mg2+ deficiency.
Considering these limitations, we resorted to an alternative experimental model that allowed us to investigate whether Mg2+ deficiency in isolated cells affects mitochondrial function. We found that CRISPR/Cas9-mediated inactivation of TRPM7 in the genetically tractable HAP1 cell line (human haploid leukaemia cells) results in Mg2+ deficiency and, consequently, a Mg2+-dependent proliferation defect (new Figure 6—figure supplement 3). Remarkably, we observed that TRPM7-deficient HAP1 cells display reduced ATP levels at resting conditions. Finally, we show that the respiration rate of TRPM7-deficient HAP1 cells was markedly supressed when compared to control cells (new Figure 6—figure supplement 3). Taken together, we conclude that Mg2+ deficiency of HAP1 cells recapitulates our key findings in Trpm6-deficient mice. These new findings are now shown in Figure 6—figure supplement 3.
2) The authors propose that TRPM6 regulates Mg2+ transport by reducing the MgATP-dependent inhibition of heteromeric TRPM6/7 channels. First, the evidence for this rests on whether the inhibition curves in Figure 6B and C are significantly different between WT and KO animals; this should be evaluated. In addition, it is not clear that MgATP in intact cells is in the required range that would differentially affect the current in the WT vs. TRPM6 groups (Figure 6B); either the ATP level should be measured, or a suitable reference provided. Finally, another group (Zhang, Yu et al., 2014) reported that TRPM6 expression by itself produced currents, and TRPM6 and M7 coexpression generated currents that were essentially insensitive to MgATP inhibition. This should be discussed in the context of the results shown in Figure 6, where TRPM7 KO completely eliminated current, and wild type and TRPM6 currents are both inhibited by MgATP.
We agree with the referees and introduced several changes in the manuscript to address their concerns. We re-analyzed the inhibition curves for Mg2+ and MgATP using a nonlinear (least-squares) regression fitting and F-test (GraphPad Prism 6.0 software) to address the questions: (i) Is the dose-response dataset of KO cells is statistically different from that in control cells? And (ii) are IC50 values distinct for the inhibitory curves obtained for KO and control cells? This analysis supported our initial conclusion that Mg2+ elicits similar inhibitory effects on the currents in WT and KO cells, whereas the inhibitory dose-response curves for MgATP were statistically different between KO and control cells. This information is now included in the Results (subsection “TRPM6 cooperates with TRPM7 to regulate divalent cation currents”) and Methods (subsection “Isolation and characterization of mouse trophoblast stem (TS) cells”) sections.
As suggested, we referenced the physiological levels of cytosolic MgATP (subsection “TRPM6 cooperates with TRPM7 to regulate divalent cation currents”) to support the notion that intracellular MgATP can differentially affect ion currents in control and KO cells. In addition, we discuss the work of Zhang et al. (Discussion, fifth paragraph) reporting the functional analysis of recombinant TRPM6 and TRPM7 co-transfected at the ratio 1:1 in HEK 293 cells. A key finding of this study was that recombinant TRPM6 offset the sensitivity of the TRPM7/M6 complex to cytosolic MgATP. Assuming that native currents in WT cells are mediated by TRPM7 homomers and TRPM6/M7 heterotetramers, it is imaginable that genetic ablation of the TRPM6/M7 fraction would only partially reduce the inhibitory effect of MgATP on whole-cell currents. Hence, we conclude that the observations of Zhang et al. are compatible with our results. Another finding from Zhang et al. is that overexpression of TRPM6 homomers in HEK 293 cells results in expression of a functional channel only if TRPM6 cDNA was expressed by the pCINeo-IRES-GFP vector, whereas the same cDNA sequence placed in various other expression plasmids did not produce active TRPM6 channels. This feature of TRPM6 cDNA appears to be unique among TRP channels, and as the nature of this observation is not currently understood, some caution is required in interpreting the results. Therefore, we have concentrated on the functional analysis of endogenous TRPM6 channels in primary cells for defining the cellular role of TRPM6 rather than on overexpression data.
Finally, we would like to emphasize that according to our model, TRPM6 regulates Mg2+ uptake by two means: (i) by increasing amplitudes of TRPM7-like currents and (ii) by relieving TRPM7 from the negative feedback by MgATP. To better illustrate the first mechanism, we now include a new Figure 6B, showing measurements of native currents in the absence of external divalent cations. This approach is widely used for a quantitative assessment of otherwise very small inward currents of TRPM7/M6, since external divalent cations elicit a strong permeation block of the channel at physiological membrane potentials. As expected, these experiments showed that current amplitudes recorded in KO cells were substantially lower than in control cells.https://doi.org/10.7554/eLife.20914.030
- Vladimir Chubanov
- Thomas Gudermann
- Emiel PC van der Vorst
- Christian Weber
- Susanna Zierler
- Susanna Zierler
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
We thank Jackson Laboratory Sacramento for the help with whole-life dietary treatments of mice. We thank David Clapham for providing Trpm7fl/fl mice; Ilia Rodushkin for the support in ICP-MS; Marc Freichel and Petra Weißgerber for their help to isolate TS cells; Fabian Bamberg and Mike Notohamiprodjo for the help with X-ray imaging of mice; Sabine Schmitt for the help with high-resolution respirometry. We thank Renate Heilmair and Joanna Zaißerer for technical assistance. VC, SZ and TG were supported by the Deutsche Forschungsgemeinschaft, TRR 152. WJ and AS were supported by the German Federal Ministry of Education and Research (BMBF, DZD). SZ was supported by Marie-Curie Fellowship (REA) FP7-PEOPLE-2012-CIG. EPCV and WC were supported by the Deutsche Forschungsgemeinschaft (SFB1123-A1) and German Centre for Cardiovascular Research (MHA VD1.2).
Animal experimentation: Experiments involving animals were done in accordance with the EU Animal Welfare Act and were approved by the local councils on animal care (permit No 55.2-1-54-2532-134-13 from Government of Oberbayern, Germany, and permit No 2347-15-2014 from State Ministry of Brandenburg, Germany).
- Richard S Lewis, Stanford University School of Medicine, United States
© 2016, Chubanov et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.