(A) Mean survival ages of B6C3F1 mice maintained at a control diet (Control, n = 335), under dietary restriction (DR, n = 60), or supplemented by Mg(CH3COO)2 (MgAc, n = 15), Mg(OH)2 (n = 15), MgCl2 (n = 15) and CaCl2 (n = 15) in drinking water as outlined in Table 2. Pooled Mg shows results for all Mg2+ supplemented mice pooled within a common group (n = 45). The obtained survival distributions were analysed by the MATLAB computing environment to calculate mean lifespans and corresponding P-values: ***-p≤0.001; **-p≤0.01; *-p≤0.05; n.s. – not significantly different. Alternatively, survival data of control mice versus individually treated groups were assessed by log-rank test: ###-p≤0.001; ##-p≤0.01; #-p≤0.05; n.s. – not significantly different. (B) Kaplan-Meier survival distributions of B6C3F1 mice maintained on control diet (Control), Mg2+ supplemented groups (MgAc, MgCl2, Mg(OH)2) or mice under dietary restriction (DR). (C) Body weights (mean+/-SEM) of control and nutritionally fortified mice studied in (A). ***-p≤0.001; **-p≤0.01; *-p≤0.05; n.s. – not significantly different (one-way ANOVA). n – number of mice examined.