(A) Mirrored barplot of the mean normalized RNAseq expression values for the OR genes in male (dark blue, top) and female (light blue, bottom) B6 animals (n = 3). (B) Scatter plot for the same data, with the Spearman’s correlation (rho) indicating a strong correlation. The red line is the 1:1 diagonal. Significantly differentially expressed (DE) OR genes are represented in blue, non-DE genes are in black. (C) Same as in (A) but with the average B6 expression in blue (both males and females, n = 6) compared to the corresponding 129 expression values in yellow (n = 3). (D) Corresponding scatter plot, with the significant DE genes in green. (E) Same as in (A) but comparing the B6 expression in blue (n = 6) to the CAST abundances in red (n = 3). (F) Corresponding scatter plot, with DE genes in purple. (G) Venn diagram illustrating the intersection of DE OR genes between the pairwise comparisons of the three strains. (H) An example of an OR gene, Olfr6, that is DE in all strain comparisons. (I) Representative in situ hybridizations (ISH) on coronal slices of B6 and 129 MOEs for two OR genes, Olfr31 and Olfr736, that are DE between these strains. (J) The quantification of OSNs expressing each OR gene in B6 (blue) and 129 (yellow) are plotted alongside the corresponding RNAseq counts. The log2 fold-changes between the strains are indicated. (K) Fold-change between the strains obtained from ISH data (x-axis) or RNAseq counts (y-axis) for four DE OR genes expressed higher in B6 (blue), four expressed higher in 129 (yellow) and one expressed at equivalent levels in both strains (grey); these include Olfr31 and Olfr736. The line is the linear regression and the Spearman’s coefficient (rho) indicates a strong correlation between OSN and RNAseq counts. See also Figure 2—figure supplement 1.