(A) Immunoblot validation of cytoplasmic tyrosine kinases (ABL, BCR-ABL, ITK, SYK, TEC, TYK2, ZAP70, BLK, FGR, FYN, YES, LCK, LYN), serine/threonine kinases (B-RAF, C-RAF) and small GTPase RAS expression and activation in 293T cells. Cells transfected with empty plasmid serve as transfection control. Actin serves as the loading control. Control, untransfected cells. WT, wild-type RAS, V12, active RAS mutant. Two variants (p190 and p210) of oncogenic BCR-ABL are shown. CAAX, active C-RAF variant; V600E, oncogenic B-RAF mutant. (B) Immunoblot analysis of EGR1 protein induction in 293T cells, following 24 hr of expression of the indicated proteins in 293T cells. (C) Compilation of the data generated in (B). Green, expression of given protein induces EGR1; red, no EGR1 protein induction; * kinases that induced EGR1 but were not found autophosphorylated; ¶ kinases which did not induce EGR1 but were found autophosphorylated. Data show a representative experiment for three independent experiments.