Functional double dissociation within the entorhinal cortex for visual scene-dependent choice behavior
- Seoul National University, Korea
Figures
Figure 1
Bilateral cannula implantations in the LEC and MEC within the same rat and histological results.
(A) Custom-made 3D-printed cannula complex. (B) Representative photomicrographs showing the cannula tracks in the LEC (upper left) and MEC (upper right) from the same rat. The numbers denote the relative positions from bregma (mm). (C) Locations of cannula tips of all rats used in the SSC task (n = 8). (D) Locations of cannula tips of all rats used in the SNSC task (n = 9). For LEC cannula locations, red circles indicate medially placed cannula positions in the LEC and blue circles indicate laterally placed cannula positions. The numbers besides the sections denote relative positions of the sections from bregma (mm).
Figure 2
Visual scene-based and tactile cue-based spatial choice tasks.
(A) Visual scene stimuli that were used in the SSC task. The two scenes were associated with the left turn, and the other two scenes were associated with the right turn. (B) Post-surgical performance in the SSC task. The MEC-MUS group exhibited significant performance deficits compared to the EC-aCSF and LEC-MUS groups. (C) Performance in the tactile cue-based choice task in the T-maze.
-
Figure 2—source data 1
(SSC).
Source data for performance in the SSC task (Figure 2B).
- https://doi.org/10.7554/eLife.21543.004
-
Figure 2—source data 2
(Tactile).
Source data for performance in the tactile-based spatial choice task (Figure 2C).
- https://doi.org/10.7554/eLife.21543.005
Figure 3
Visual scene-dependent nonspatial memory task and behavioral performance.
(A) Visual scene stimuli and their associated choice responses in the SNSC task. (B) Post-surgical performance in the SNSC task. The LEC-MUS condition induced significant performance deficits compared to the EC-aCSF condition. **p<0.01, ***p<0.0001. (C) Rats with the LEC cannulae implanted more medially showed bigger deficits in performance than those with more lateral LEC cannulae, whereas those rats were not different from each other when aCSF was injected.
-
Figure 3—source data 1
(SNSC).
Source data for performance in the SNSC task (Figure 3B and C).
- https://doi.org/10.7554/eLife.21543.008
Figure 4
Double dissociation between the LEC and MEC in the SSC and SNSC tasks.
Ordinate represents the amount of performance difference between aCSF and MUS conditions. *p<0.05, **p<0.001.
Figure 5
Illustration of the object-based choice task and post-surgical performance.
(A) In the task, the rats were trained to respond either by digging or pushing the jar based on the magnetic object cue attached to the jar. (B) Post-surgical performance on the task. No significant differences were found between animals in either drug condition.
-
Figure 5—source data 1
(Object).
Source data for performance in the object memory task (Figure 5B).
- https://doi.org/10.7554/eLife.21543.012
Videos
Video 1
SSC task.
Normal performance of rats in the SSC task. Sample trials, each associated with zebra stripes, bamboos, mountains, or pebbles scene are shown in the video.
Video 2
SNSC task.
Performance of rats when injected with aCSF in the EC versus MUS in the LEC in the SNSC task. Scenes are not shown in the video.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Functional double dissociation within the entorhinal cortex for visual scene-dependent choice behavior
eLife 6:e21543.
https://doi.org/10.7554/eLife.21543
