Molecular architecture of the 90S small subunit pre-ribosome
- School of Life Sciences, Tsinghua University, China
- Chinese Academy of Sciences, China
- National Institute of Biological Sciences, China
Abstract
Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately 5 megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. Majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.
Article and author information
Author details
Funding
National Natural Science Foundation of China (91540201; 31430024; 31325007)
- Keqiong Ye
Chinese Academy of Sciences (XDB08010203)
- Keqiong Ye
Ministry of Science and Technology of the People's Republic of China (2014CB84980001)
- Meng-Qiu Dong
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Sun et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 5,269
- views
-
- 1,121
- downloads
-
- 113
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 113
- citations for umbrella DOI https://doi.org/10.7554/eLife.22086
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Molecular architecture of the 90S small subunit pre-ribosome
eLife 6:e22086.
https://doi.org/10.7554/eLife.22086
