(A) Histograms for each neuron showing the distribution of neurite lengths across all soma-to-tip paths. Neurite path lengths were measured in three-dimensional skeletal reconstructions generated in KNOSSOS from high-resolution confocal image stacks of Lucifer yellow neuronal dye-fills. There were no cell-type-specific differences in neurite length distributions (ANOVA; [F(3,12)=1.48, p=0.2698]; Tukey HSD). Means are indicated by solid lines; medians are indicated with dashed lines; darker shaded regions indicate 25–75% confidence intervals. Neurite diameters were randomly sampled across primary (1°), secondary (2°), tertiary (3°), and terminating (tip) neurites and manually measured using ImageJ. (B) Illustration of 1° branch (red) and individual 2° (cyan) and 3° (white) branches on one neuron (z-projection of confocal micrograph at 60x magnification, neuron pseudo-colored in yellow). (C) Scatter plots of neurite diameters as a function of neurite order within neuron types show decreasing neurite diameters as a function of neurite order. ANOVA results are: [F(3,149)=14.77, p<<0.001; Tukey HSD)], [F(3,152)=67.16, p<<0.001; Tukey HSD)], [F(3,152)=33.92, p<<0.001; Tukey HSD)], [F(3,146)=16.43, p<<0.001; Tukey HSD)], for GM, LG, LP, and PD, respectively. (D) Scatter plot shows all measured diameters, across all cell types, as a function of neurite order. When all cell types are pooled are together, diameter decreases as a function of neurite order (ANOVA [F(3,611)=89.17, p<<0.001]; Tukey HSD; indicated with letters). ANOVA analyses within each neurite order, across cell types, revealed no significant differences across cell types. For C and D pooled mean ± SD within each neurite order indicated with black lines. In A, C, and D neuron types (n = 4 of each) are color-coded as in key.