(A) Overlay of the SNX5-PX:IncE structure with the crystal structure of the PX domain from p40phox bound to phosphatidylinositol 3-phosphate (PDB ID 1H6H) (Bravo et al., 2001). SNX5-PX and IncE are colored green and gold, respectively. The PX domain from p40phox is colored pink and the phosphatidylinositol 3-phosphate is shown as sticks with carbons colored pink. (B) IncE does not affect SNX5 binding to immobilized lipids. Purified SNX5-PX20-180 alone or in complex with IncE108-120 was incubated with membranes containing various phospholipids as described in Methods. SNX5 binding was detected using anti-SNX5 antibody. A blot representative of two independent biological experiments is shown. LPA, lyosophophatidic acid. LPC, lysophosphocholine. PtdIns, phosphatidylinositol. PI(3)P, phosphatidylinositol (3) phosphate. PI(4)P, phosphatidylinositol (4) phosphate. PI(5)P, phosphatidylinositol (5) phosphate. PE, phosphatidylethanolamine. PC, phosphatidylcholine. S1P, sphingosine 1-phosphate. PI(3,4)P2, phosphatidylinositol (3,4) bisphosphate. PI(3,5)P2, phosphatidylinositol (3,5) bisphosphate. PI(4,5)P2, phosphatidylinositol (4,5) bisphosphate. PI(3,4,5)P3, phosphatidylinositol (3,4,5) triphosphate. PA, phosphatidic acid. PS, phosphatidylserine.