1. Biochemistry and Chemical Biology
  2. Microbiology and Infectious Disease
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Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens

  1. Qing Yan
  2. Benjamin Philmus
  3. Jeff H Chang
  4. Joyce E Loper  Is a corresponding author
  1. Oregon State University, United States
Research Article
  • Cited 20
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Cite this article as: eLife 2017;6:e22835 doi: 10.7554/eLife.22835

Abstract

Metabolic co-regulation between biosynthetic pathways for secondary metabolites is common in microbes and can play an important role in microbial interactions. Here, we describe a novel mechanism of metabolic co-regulation in which an intermediate in one pathway is converted into signals that activate a second pathway. Our study focused on the co-regulation of 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin, two antimicrobial metabolites produced by the soil bacterium Pseudomonas protegens. We show that an intermediate in DAPG biosynthesis, phloroglucinol, is transformed by a halogenase encoded in the pyoluteorin gene cluster into mono- and di-chlorinated phloroglucinols. The chlorinated phloroglucinols function as intra- and inter-cellular signals that induce the expression of pyoluteorin biosynthetic genes, pyoluteorin production, and pyoluteorin-mediated inhibition of the plant-pathogenic bacterium Erwinia amylovora. This metabolic co-regulation provides a strategy for P. protegens to optimize the deployment of secondary metabolites with distinct roles in cooperative and competitive microbial interactions.

Article and author information

Author details

  1. Qing Yan

    Department of Botany and Plant Pathology, Oregon State University, Corvallis, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Benjamin Philmus

    Department of Pharmaceutical Sciences, Oregon State University, Corvallis, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Jeff H Chang

    Department of Botany and Plant Pathology, Oregon State University, Corvallis, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Joyce E Loper

    Department of Botany and Plant Pathology, Oregon State University, Corvallis, United States
    For correspondence
    joyce.loper@oregonstate.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3501-5969

Funding

National Institute of Food and Agriculture (NRI 2011-67019-30192)

  • Jeff H Chang
  • Joyce E Loper

Oregon State University (Research startup funds)

  • Benjamin Philmus

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Jon Clardy, Harvard Medical School, United States

Publication history

  1. Received: October 31, 2016
  2. Accepted: February 16, 2017
  3. Accepted Manuscript published: March 6, 2017 (version 1)
  4. Version of Record published: April 18, 2017 (version 2)

Copyright

© 2017, Yan et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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