(A) Representative images of CFs fixed after 24 hr stimulations and stained for collagen I (green) and DAPI (blue). Scale bars = 20 μm. An intense perinuclear staining is observed in CFs stimulated either by 8% mechanical strain or by HX. (B) Quantitative fluorescence intensity analyses on Collagen I staining plotted as graph. Collagen I staining is increased by HX, 8% strain or NX conditions. Data collected from one cell donor, two independent experiments, minimum number of experimental replicates n = 4, technical replicates (multiple images per replicate) n = 3. Detected amounts of MMP-2 (C) and MMP-3 (D) in supernatants of CFs. Both MMP-2 and MMP-3 expression is significantly increased in combined HX and mechanical strain stimulation. Protein secretion data collected from one cell donor, two independent experiments, minimum number of experimental replicates n = 4, technical replicates n = 2. White histograms correspond to 0% strain conditions, grey histograms to 2% strain conditions and black histograms to 8% strain conditions. Two-way ANOVA test was performed for all groups. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. One outlier measurement in MMP-3 expression was detected by performing Grubb’s test (α = 0.05; p<0.05) and removed from the analysis.