mTORC1 in AGRP neurons integrates exteroceptive and interoceptive food-related cues in the modulation of adaptive energy expenditure in mice

  1. Luke K Burke
  2. Tamana Darwish
  3. Althea R Cavanaugh
  4. Sam Virtue
  5. Emma Roth
  6. Joanna Morro
  7. Shun-Mei Liu
  8. Jing Xia
  9. Jeffrey W Dalley
  10. Keith Burling
  11. Streamson Chua
  12. Toni Vidal-Puig
  13. Gary J Schwartz
  14. Clémence Blouet  Is a corresponding author
  1. University of Cambridge, United Kingdom
  2. The Albert Einstein College of Medicine, United States
6 figures and 1 additional file

Figures

Figure 1 with 1 supplement
AGRP neurons control iBAT thermogenesis.

iBAT temperature (a), energy expenditure (b), locomotor activity (c), Norepinephrine turnover (n = 8) (d) and phosphorylation levels of lipolytic pathways in the iBAT (d) following chemogenetic …

https://doi.org/10.7554/eLife.22848.003
Figure 1—figure supplement 1
AGRP neurons control iBAT thermogenesis.

Confirmation of successful AAV-targeting using mCherry staining - Scale bar 150 μm (a). Confirmation of increased food intake upon CNO administration (b) iBAT temperature in response to local …

https://doi.org/10.7554/eLife.22848.004
Figure 2 with 1 supplement
Food availability and sensory cues of food availability modulate the AGRP-iBAT circuit.

iBAT temperature (a), energy expenditure (b) and locomotor activity (c) following chemogenetic activation of AGRP neurons in the presence of food (DESIGN 2). iBAT temperature (d), energy expenditure …

https://doi.org/10.7554/eLife.22848.005
Figure 2—figure supplement 1
Food availability and sensory cues of food availability modulate the AGRP-iBAT circuit.

iBAT temperature (a) and energy expenditure (b) following chemogenetic activation of AGRP neurons and exposure to a novel empty container. Data are mean ± SEM.

https://doi.org/10.7554/eLife.22848.006
Figure 3 with 1 supplement
mTOR signaling in AGRP neurons senses endogenous and environmental signals of energy availability, regulates AGRP activity and iBAT thermogenesis.

Activation levels of mTOR in Npy-GFP neurons following a 18 hr fast and 1 hr chow re-feed (a, d, n = 5), and a 48 hr transition from chow to HF diet (b, e, n = 6), and a 18 hr fast followed by a 30 …

https://doi.org/10.7554/eLife.22848.007
Figure 3—figure supplement 1
mTOR signaling in AGRP neurons senses endogenous and environmental signals of energy availability, regulates AGRP activity and iBAT thermogenesis.

iBAT temperature (a) and energy expenditure (b) in mice fasted for 19 hr and refed for 60 min. p-mTOR immunofluorescent staining in mouse hypothalamic brain sections at the rostrocaudal level of the …

https://doi.org/10.7554/eLife.22848.008
Figure 4 with 1 supplement
Metabolic phenotyping of mice expressing constitutively active mutant of mTORC1 effector p70 S6 kinase one in Agrp-IRES-cre (Agrp-CA-S6) or wild-type (WT-CA-S6) littermates.

Food intake (a), body weight gain on high-fat diet (b), energy expenditure (c), RER (d), locomotor activity (e), iBAT temperature (f), iBAT thermogenic gene expression (g), iBAT temperature …

https://doi.org/10.7554/eLife.22848.009
Figure 4—figure supplement 1
Metabolic phenotyping of mice expressing constitutively active mutant of mTORC1 effector p70 S6 kinase one in Agrp-IRES-cre (Agrp-CA-S6) or wild-type (WT-CA-S6) littermates.

Ha (a) and p-rpS6 (b) immunostaining in Agrp-IRES-cre;Npy-GFP mice infected with the pCDH-CMV-FLEX-HA-S6K1-F5a lentivirus. Body weight gain (c) fat mass (d) and glucose tolerance (d) of Agrp-CA-S6 …

https://doi.org/10.7554/eLife.22848.010
Figure 5 with 1 supplement
Metabolic phenotyping of mice expressing dominant negative mutant of mTORC1 effector p70 S6 kinase 1 (S6K1) in Agrp-IRES-cre (Agrp-DN-S6) or wild-type (WT-DN-S6).

Energy expenditure (a), locomotor activity (b), iBAT temperature (c), iBAT thermogenic gene expression (d), body weight gain during initial switch to HF diet (e), food intake (f), body weight loss …

https://doi.org/10.7554/eLife.22848.011
Figure 5—figure supplement 1
Metabolic phenotyping of mice expressing dominant negative mutant of mTORC1 effector p70 S6 kinase 1 (S6K1) in Agrp-IRES-cre(Agrp-DN-S6) or wild-type (WT-DN-S6).

Ha (a) and p-rpS6 (b) immunostaining in Agrp-IRES-cre;Npy-GFP mice infected with the pCDH-CMV-FLEX-HA-S6K1-KR lentivirus. Body weight gain on chow (c) and RER in Agrp-DN-S6 and WT-DN-S6 mice. Data …

https://doi.org/10.7554/eLife.22848.012
Leptin engages mTORC1 signaling in AGRP neurons to regulate thermogenesis.

Expression of p-mTORC1 (a, c) and colocalization of p-rpS6 and p-STAT3 (b, d) in Npy-GFP neurons following systemic leptin treatment. Leptin’s thermogenic effects in WT-CA-S6 and Agrp-CA-S6 (e), and …

https://doi.org/10.7554/eLife.22848.013

Additional files

Supplementary file 1

Real time PCR oligonucleotide sequences.

https://doi.org/10.7554/eLife.22848.014

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