iBAT temperature (a), energy expenditure (b), locomotor activity (c), Norepinephrine turnover (n = 8) (d) and phosphorylation levels of lipolytic pathways in the iBAT (d) following chemogenetic …
Confirmation of successful AAV-targeting using mCherry staining - Scale bar 150 μm (a). Confirmation of increased food intake upon CNO administration (b) iBAT temperature in response to local …
iBAT temperature (a), energy expenditure (b) and locomotor activity (c) following chemogenetic activation of AGRP neurons in the presence of food (DESIGN 2). iBAT temperature (d), energy expenditure …
iBAT temperature (a) and energy expenditure (b) following chemogenetic activation of AGRP neurons and exposure to a novel empty container. Data are mean ± SEM.
Activation levels of mTOR in Npy-GFP neurons following a 18 hr fast and 1 hr chow re-feed (a, d, n = 5), and a 48 hr transition from chow to HF diet (b, e, n = 6), and a 18 hr fast followed by a 30 …
iBAT temperature (a) and energy expenditure (b) in mice fasted for 19 hr and refed for 60 min. p-mTOR immunofluorescent staining in mouse hypothalamic brain sections at the rostrocaudal level of the …
Food intake (a), body weight gain on high-fat diet (b), energy expenditure (c), RER (d), locomotor activity (e), iBAT temperature (f), iBAT thermogenic gene expression (g), iBAT temperature …
Ha (a) and p-rpS6 (b) immunostaining in Agrp-IRES-cre;Npy-GFP mice infected with the pCDH-CMV-FLEX-HA-S6K1-F5a lentivirus. Body weight gain (c) fat mass (d) and glucose tolerance (d) of Agrp-CA-S6 …
Energy expenditure (a), locomotor activity (b), iBAT temperature (c), iBAT thermogenic gene expression (d), body weight gain during initial switch to HF diet (e), food intake (f), body weight loss …
Ha (a) and p-rpS6 (b) immunostaining in Agrp-IRES-cre;Npy-GFP mice infected with the pCDH-CMV-FLEX-HA-S6K1-KR lentivirus. Body weight gain on chow (c) and RER in Agrp-DN-S6 and WT-DN-S6 mice. Data …
Expression of p-mTORC1 (a, c) and colocalization of p-rpS6 and p-STAT3 (b, d) in Npy-GFP neurons following systemic leptin treatment. Leptin’s thermogenic effects in WT-CA-S6 and Agrp-CA-S6 (e), and …
Real time PCR oligonucleotide sequences.