(a) Schematic representation of experimental procedure: lethally irradiated CD45.2+ recipients mice were co-injected with sorted scAT-LSK and total BM cells isolated respectively from CD45.1 and CD45.2 expressing donor mice, and were then fed a normal Chow (NC) or a high fat diet (HFD) for 12 weeks. Metabolic profile was investigated in chimeric mice by (b) body weight, (c) scAT weight, (d and insert) IPGTT and AUC, (e) quantification of fasted insulin and (f) scAT-glucose uptake (ng/mg.min). (g) Quantification of ATM identified as F4/80+/MHCII+ in the scAT. (h) Expression of genes encoding for inflammatory cytokines analyzed by qRT-PCR in the scAT and expressed as a percent of control values obtained in NC mice. (i–n) Flow cytometry was performed on SVF and BM cells from NC and HFD mice to identify CD45.1+ populations. Representative histograms of CD45.1+ cells in the scAT (i), and the BM (j). (k) Total chimerism in the scAT, pgAT, liver, muscles and BM expressed as percent of total CD45+ cells. (l) Representative dot plots of flow cytometry analysis showing CD11c+/F4/80+/MHCII+ cells in a CD45.1+ cell population gated on singlet live cells. Quantification of scAT-LSK-derived (m) and BM-derived (n) pro-inflammatory (MHCII+/F4/80+/CD11c+) and anti-inflammatory scATM populations (MHCII+/F4/80+/CD206+) expressed in absolute numbers. (o) Correlation between CD45.1+ ATM content (expressed in % of SVF) and AUC. Results are expressed as mean ± sem of 4 to 26 individual animals in control (white symbols) and HFD (grey symbols) groups. Comparisons between groups were made with the nonparametric Mann-Whitney test. *p<0.05; **p<0.01; ***p<0.001.