(A) qPCR analysis of Ereg, Krt5, Krt14, and Yap expression in E13.5 WT SMGs treated with control siRNA or siRNA targeting Ereg. The average of three SMGs from different litters is shown +S.E.M. [one sample t-test: *p<0.01, **p<0.001, ***p<0.0001]. (B) E13.5 WT SMGs were dissected and cultured for 24 hr in the presence of control siRNA or Epiregulin siRNA and analyzed by phase-contrast and IF for Krt14 (K14, magenta), Krt5 (K5, green), Krt19 (K19, red), TuJ1 (yellow), and F-actin (white, Phalloidin). DAPI was used to mark the nuclei (blue). Scale = 100 μm. All images represent observations made from a minimum of three biological repeats.