1. Computational and Systems Biology
  2. Microbiology and Infectious Disease
Download icon

The cyanobacterial circadian clock follows midday in vivo and in vitro

  1. Eugene Leypunskiy
  2. Jenny Lin
  3. Haneul Yoo
  4. UnJin Lee
  5. Aaron R Dinner  Is a corresponding author
  6. Michael J Rust  Is a corresponding author
  1. University of Chicago, United States
Research Article
  • Cited 7
  • Views 1,937
  • Annotations
Cite this article as: eLife 2017;6:e23539 doi: 10.7554/eLife.23539

Abstract

Circadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run, but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cycles are not well understood. Here we report experimental platforms for driving the cyanobacterial circadian clock both in vivo and in vitro. We find that the phase of the circadian rhythm follows a simple scaling law in light-dark cycles, tracking midday across conditions with variable day length. The core biochemical oscillator comprised of the Kai proteins behaves similarly when driven by metabolic pulses in vitro, indicating that such dynamics are intrinsic to these proteins. We develop a general mathematical framework based on instantaneous transformation of the clock cycle by external cues, and it successfully predicts clock behavior under many cycling environments.

Article and author information

Author details

  1. Eugene Leypunskiy

    Graduate Program in Biophysical Sciences, University of Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Jenny Lin

    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Haneul Yoo

    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. UnJin Lee

    Department of Ecology and Evolution, University of Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Aaron R Dinner

    Graduate Program in Biophysical Sciences, University of Chicago, Chicago, United States
    For correspondence
    dinner@uchicago.edu
    Competing interests
    The authors declare that no competing interests exist.
  6. Michael J Rust

    Graduate Program in Biophysical Sciences, University of Chicago, Chicago, United States
    For correspondence
    mrust@uchicago.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7207-4020

Funding

Pew Charitable Trusts

  • Michael J Rust

National Institute of General Medical Sciences (R01GM107369-01)

  • Michael J Rust

National Science Foundation (PHY-1305542)

  • Aaron R Dinner

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Naama Barkai, Weizmann Institute of Science, Israel

Publication history

  1. Received: November 22, 2016
  2. Accepted: July 6, 2017
  3. Accepted Manuscript published: July 7, 2017 (version 1)
  4. Version of Record published: September 19, 2017 (version 2)

Copyright

© 2017, Leypunskiy et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,937
    Page views
  • 426
    Downloads
  • 7
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Chromosomes and Gene Expression
    2. Computational and Systems Biology
    Andrew N Holding et al.
    Short Report
    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease
    Kevin D'hoe et al.
    Research Article Updated