(A) Heatmap of global gene expression (with log2 fold change >1.5 and adjusted B-H p-value < 0.05). Numbers denote ID of a specific embryo. (B) Principal Component Analysis (PCA) of the RNA-Seq samples: In vivo embryos (IV, red), Natur-IVF (N, green) and C-IVF (C, blue). Numbers denote ID of specific embryos. (C) Venn diagram with DEGs (Figure 3—source data 1). *, #, § denotes DEGs exclusive for C-IVF, Natur-IVF and In vivo, respectively (Figure 3—source data 2). (D) Heat map of gene expression of key genes associated with embryo development/differentiation, epigenetic reprogramming, cell cycle/cell growth, gene expression and imprinting.