(A) Confocal microscopy images of YFP-βC1 in mesophyll cells of N. benthamiana leaves. YFP-βC1 was transiently expressed in non-silenced control (TRV alone), ATG5 or ATG7 silenced plants. The confocal microscope images of mesophyll cells were taken at 60 hpi. (B) Western blot analyses of YFP-βC1 construct from the same experiments as in (A). Level of the fusion protein, YFP-βC1, was detected with anti-GFP polyclonal antibody. (C) Silencing of either ATG5 or ATG7 enhanced the accumulation of HA-βC1, but not HA-βC1V32A. Each expression constructs were agroinfiltrated into N. benthamiana leaf. At 60 hpi leaf lysates were separated by SDS-PAGE and fusion proteins were detected by anti-GFP or anti-HA antibodies.