(A–C) Western blotting for γ-H2AX, H2AX, phosphorylated KAP-1 (p-KAP-1) and KAP-1 in whole cell lysates from (A) wild type (WT) BMDMs after no stimulation (-) or stimulation (+) with LPS, IFN-γ, or …
Southern blot analysis of genomic DNA digested with KpnI and hybridized to the 3’ ATM probe from BMDMs from AtmC/C, Atm-/-and Scid:AtmC/C:Lyz2Cre/+ mice. Arrows indicate the conditional (C) and …
Flow cytometric analysis of BrdU incorporation (y axis) and DNA content (7AAD; x axis) was performed in Lyz2Cre/+ and Scid: AtmC/C: Lyz2Cre/+ BMDMs after L. monocytogenes infection for 16 hr. …
(A) Neutral Comet assay and Olive Tail Moment quantification of BMDMs left untreated (UT) or after infection with L. m. or LPS + IFN-γ for 12 hr. (B) Immunofluorescence for L. m. (green) and γ-H2AX …
(A) Additional representative images of immunofluorescence for L. m. (green) and γ-H2AX (red) in WT BMDMs 9 hr. post-infection. Nuclei are revealed by DAPI (blue). (B) Quantification of γ-H2AX foci …
(A,B) Western blotting for γ-H2AX and H2AX in whole cell lysates from (A) WT or Myd88-/- BMDMs after no infection (-) or infection (+) with L. monoctytogenes (L. m.) for 24 hr. (B) WT BMDMs after no …
(A) Nitrite concentration in culture supernatants collected from WT and Ifnar1-/- BMDMs after no infection (-) or infection (+) with L. m. for 24 hr. in the presence or absence of IFN-γ. Data are a …
Flow cytometric analysis of BrdU incorporation (y axis) and DNA content (7AAD; x axis) was performed in BMDMs left untreated or after stimulation with IFN-β (100 U/mL), bleomycin (1 ug/mL), or …
Western blotting for Mre11, Nbs1, Rad50, Ku70, Ku80, ATM, ATR, DNA-PKcs, and H2AX in untreated (-) WT mouse embryonic fibroblasts (MEFs) or BMDMs after no stimulation (-) or stimulation (+) with …
(A) Heat map showing genes that are significantly up-regulated (≥4 fold, adj. p-value<0.001) in L. m.-infected Lyz2Cre/+and Scid:Atm C/C:Lyz2Cre/+ BMDMs relative to no infection. Red indicates …
Gene expression changes induced by L. monocytogenes infection.
Normalized log2 probe values for genes up-regulated (≥4 fold, adj. p-value<0.001) in L. m.-infected Lyz2Cre/+and Scid:Atm C/C:Lyz2Cre/+ BMDMs relative to uninfected cells. The fold-change between infected Scid:Atm C/C:Lyz2Cre/+-and infected Lyz2Cre/+ cells for each gene is also shown.
(A) IL-1β protein concentration as determined by ELISA in supernatants from uninfected (-) and L. m.-infected (+) Scid:AtmC/C:Lyz2Cre/+ and Lyz2Cre/+ BMDMs 12 and 24 hr. post-infection. Data are …
Flow cytometric analysis of mitochondrial superoxide levels in live BMDM from Lyz2Cre/+, AtmC/C: Lyz2Cre/+, and Scid: AtmC/C: Lyz2Cre/+ mice. Superoxide is detected with the fluorogenic dye MitoSOX …
(A–D) Quantitative RT- PCR analysis of (A) pro Il-1β, (B) Asc, (C) Nlrp3, and (D) Aim2 gene expression in Scid: AtmC/C: Lyz2cre/+and Lyz2cre/+BMDMs infected (+) or not (-) with L. m. Data are a …
(A) IL-18 protein concentration as determined by ELISA in supernatants from uninfected (-) and L. m.-infected WT and Scid BMDMs 12 and 24 hr. post-infection. Data are mean and SEM of technical …
(A,B) Quantitative RT- PCR analysis of (A) Pro Il18 and (B) Il12p40 gene expression in WT and Scid BMDM 12 hr post- L. m. infection. Data are a compilation of three independent experiments and …