(A) Schematic of timeline for mating, sensory experience, and recording. (B) Inhibitory synaptic inputs recorded in voltage-clamped MCs from naïve, sensory-exposed, and mated mice. (C,D) Mating substantially increases mIPSC frequency. Left, cumulative interval distributions; mated < naïve and exposed groups, p=0.002 and 0.001 respectively. Right, mean frequency (F = 5.88; Fc = 3.20; p=0.005 for mated vs. naïve; ANOVA with post-hoc Tukey test; n = 18, 17, and 15 cells in 5, 5, and 6 mice respectively). (E,F) The mean amplitude of mIPSCs was not significantly changed by imprinting (F = 1.74; Fc = 3.20; p=0.19; ANOVA with post-hoc Tukey test), although distributions were significantly shifted towards smaller values in the mated vs. naïve and sensory-exposed groups (p=0.00007 and 3 × 10−7 respectively. (G) Example traces showing spontaneous EPSPs in GCs from naïve, sensory-exposed and mated mice. Rasters indicate synaptic events used for analysis. (H,I) Mating increased mean sEPSP frequency relative to both naïve and sensory-exposed animals (F = 6.64; Fc = 3.14; p=0.00037 and 0.038 for mated vs. exposed and naïve mice respectively; ANOVA with post hoc Tukey test; n = 17, 19, and 30 cells in 5, 9, and 12 mice). Interval distributions were significantly smaller for mated vs. exposed and naïve animals (p=1×10−11 and 0.0008 respectively). (J,K) Mating also increased mean sEPSP amplitude in mated vs. naïve animals. Left, cumulative distribution; right, mean amplitude (F = 3.56; Fc = 3.14; p=0.037 for naïve vs. mated, ANOVA with post hoc Tukey test). Amplitude distributions were larger for mated vs. naïve mice (p=0.04). NS, not significant; *p<0.05; **p<0.001.