Neural mechanisms of social learning in the female mouse
- Boston University, United States
- Program in Neuroscience and Behavior, Mount Holyoke College, United States
Figures
Figure 1 with 1 supplement
AOB MCs express robust, slowly emerging self-inhibition.
(A) Schematic of dendrodendritic self-inhibition pathway in MCs. (B) Left, dye-filled MC imaged after recording. S, soma; d, dendritic tufts that integrate sensory inputs; p, recording pipette. …
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Figure 1—source data 1
This spreadsheet contains the initial and final firing rates for the individual neurons shown in Figure 1G.
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
- https://doi.org/10.7554/eLife.25421.004
Figure 1—figure supplement 1
Robust self-inhibition regulates spiking of AOB MCs.
(A) Eliminating self-inhibition by blocking fast synaptic transmission increased the overall firing rate of MCs. (B) Blocking synaptic inhibition also eliminated the barrages of IPSPs that emerged …
Figure 2 with 3 supplements
Imprinting drives synaptic plasticity in both MCs and GCs.
(A) Schematic of timeline for mating, sensory experience, and recording. (B) Inhibitory synaptic inputs recorded in voltage-clamped MCs from naïve, sensory-exposed, and mated mice. (C,D) Mating …
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Figure 2—source data 1
This spreadsheet contains the mean frequency and amplitude data for the individual neurons used to generate the bar plots shown in Figure 2D and F (mitral cell mIPSCs) and 2I and 2K (granule cell mEPSCs).
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
- https://doi.org/10.7554/eLife.25421.007
Figure 2—figure supplement 1
Mating and sensory interactions during pairing.
(A) Video analysis of characteristic post-mating behavior between females and males. Top, mating typically occurred early during the pairing period (red), followed by extensive and repetitive …
Figure 2—figure supplement 2
Pharmacologically isolated inhibitory synaptic currents in MCs.
Top, inward currents recorded from voltage-clamped MCs using high-chloride pipette solution, measured in the presence of TTX, NBQX, and APV. Bottom, miniature currents were completely blocked by …
Figure 2—figure supplement 3
Synaptic effects in GCs are independent of event detection criteria.
(A) Raw data traces with rasters showing synaptic events detected using thresholds of 0.25, 0.45, and 0.65 mV. (B) Mean frequency (left) and cumulative distribution of interevent interval (right) …
Figure 3 with 1 supplement
Synaptic plasticity is uncorrelated with activation during mating.
(A) Arc-GFP labeling of AOB GCs activated by the stud male, visualized with live-tissue 2-photon imaging. Left, naïve control animal; right, mated female. (B) Fluorescence-targeted recordings of …
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Figure 3—source data 1
This spreadsheet contains the mean frequency and amplitude data for the individual neurons used to generate the bar plots shown in Figures 3C, D, I and J, comparing synaptic inputs to GFP(-) and GFP(+) neurons.
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
- https://doi.org/10.7554/eLife.25421.012
Figure 3—figure supplement 1
Mating increases fluorescent labeling in AOB and increases inhibitory synaptic input onto MCs.
(A) Cumulative histogram of Arc-GFP intensity. Mating, but not sensory exposure alone, increases labeling in GCs (p<10−45, Kolmogorov–Smirnov test; n = 14, 9, and 18 slices for naïve, …
Figure 4
Experience alters intrinsic excitability of interneurons.
(A) Representative responses to graded current injection for GCs from naïve, sensory-exposed and mated mice. (B) Current-firing plot shows a shift towards increased excitability of GCs from both …
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Figure 4—source data 1
This spreadsheet contains the resting membrane potential and firing rate data for the individual neurons used to generate the bar plots shown in Figures 4C, D and F.
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
- https://doi.org/10.7554/eLife.25421.015
Figure 5
Mating reduces the responsiveness of MCs to repetitive inputs.
(A) MC firing to an initial current stimulus is similar for naïve and mated females. (B) Firing rate profile averaged across all MCs from naïve and mated animals. (C, D) Mating has no effect initial …
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Figure 5—source data 1
This spreadsheet contains the firing rate and spike count data for mitral cells used to generate the bar plots and average data shown in Figures 5C, D and F.
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
- https://doi.org/10.7554/eLife.25421.017
Figure 6 with 2 supplements
Plasticity in MC responsiveness is specific to mating-activated neurons.
(A) Both GFP(-) and GFP(+) MCs show similar initial responses to current stimuli. (B) Mean firing rate profiles for GFP(+) and GFP(-) MCs in Fos-GFP females after mating (mean ± SEM; n = 7 and 11 …
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Figure 6—source data 1
This spreadsheet contains the firing rate and spike count data for mitral cells used to generate the bar plots and average data shown in Figures 6C, D, F and H.
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
- https://doi.org/10.7554/eLife.25421.019
Figure 6—figure supplement 1
Correlated plasticity and GFP labeling are independent of selection criteria.
(A) Arc-GFP labeling intensity for the GCs in our recording sample (green markers) superimposed on the distribution for all detectable GCs (gray line, normalized exponential fit to imaging data). …
Figure 6—figure supplement 2
Slow attenuation is absent in GFP(+) MCs labeled by sensory exposure alone.
(A) Firing rates are elevated in GFP(+) MCs relative to unlabeled neurons. (B, C) Increased firing rates for initial stimuli in GFP(+) vs. GFP(-) MCs in the absence of mating. Peak rates, 30.5 ± 2.2 …
Figure 7 with 1 supplement
Loss of MC sensitivity results from progressive membrane potential hyperpolarization.
(A) Representative MC responses to repetitive stimulation, showing initial resting potential and onset of firing for each trial. Progressive hyperpolarization was greatly enhanced in mated vs. naïve …
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Figure 7—source data 1
This spreadsheet contains the membrane potential data for mitral cells used to generate the bar plots and average data shown in Figure 7C and D.
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
- https://doi.org/10.7554/eLife.25421.023
Figure 7—figure supplement 1
MC hyperpolarization and attenuation of firing does not depend on persistent synaptic inhibition.
(A) Representative MC membrane potential response after blocking fast synaptic transmission with NBQX, APV, and bicuculline (5, 50, and 10 µM respectively). (B) Decreased MC output over the course …
