Synaptic plasticity through activation of GluA3-containing AMPA-receptors
- The Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, The Netherlands
Figures
Figure 1 with 2 supplements
cAMP increases currents of extrasynaptic GluA3-containing AMPARs.
(A) Experimental setup, example traces and averages of AMPAR amplitudes in response to AMPA puffs onto outside-out patches from WT CA1 cell bodies without (black; n=15) or with (gray; n=24) cAMP in …
Figure 1—figure supplement 1
Blocking phosphodiesterase activity is not sufficient to induce significant AMPAR potentiation.
Amplitudes of outside-out evoked recordings (from SEP-GluA3-positive neurons) in response to AMPA puffs in control condition (n=7) or in the presence of IBMX (n=3) (t-test, p=0.4). Error bars …
Figure 1—figure supplement 2
SEP-GluA3 fluorescence is pH-sensitive.
Example images and average fluorescence intensity of SEP-infected GluA3-KO at the edge of cell bodies (top: n=3) or at apical dendrites (bottom: n=2) visualized with 2-photon laser scanning …
Figure 2
cAMP increases the open-channel probability of GluA3-containing AMPARs.
(A, B) Single channel recordings under cell-attached configuration showing example traces, open probability, fraction of time spent in each open state, average conductance and conductances of each …
Figure 3 with 3 supplements
The cAMP-driven postsynaptic potentiation depends on GluA3.
(A) Example traces and average AMPA/NMDA EPSC ratios of WT neurons with (grey; n=13) or without (black; n=9) forskolin, and GluA3-KO neurons with (blue; n=8) or without (dark blue; n=8) forskolin. …
Figure 3—figure supplement 1
Forskolin increases postsynaptic AMPAR currents.
Evoked EPSC amplitude and quantal content (1/CV2) of AMPAR EPSCs, NMDAR EPSC, and AMPA/NMDA EPSC ratio upon stimulation of Schaffer collateral axons (as seen in Figure 3A) of WT CA1 neurons with …
Figure 3—figure supplement 2
Postsynaptically applied cAMP causes a GluA3-dependent synaptic potentiation.
Example traces, mEPSC frequencies and mEPSC amplitudes in the presence of IBMX of WT neurons with (n=10) or without (n=11) cAMP and of GluA3-KO neurons with (n=8) or without (n=8) cAMP in recording …
Figure 3—figure supplement 3
Sindbis viral GFP-GluA3 expression predominantly traffic into CA1 synapses in configuration of GluA2/3 heteromers.
Example traces and average rectification indices ([I-60mV – I0mV) / (I+40mV – I0mV]) were determined in uninfected (n=8) or GFP-GluA3 infected (n=8) CA1 neurons from GluA3-KO organotypic slices in …
Figure 4 with 1 supplement
cAMP does not affect GluA2/3 mobility or trafficking to synapses.
(A) Example image of a FRAP experiment: a spine from a CA1 neuron transfected with SEP-GluA3 + tdTomato obtained with two-photon microscopy immediately before, and 2, 5, 10, 20 and 30 min after …
Figure 4—figure supplement 1
Controls in FRAP experiment.
(A) Fast fluorescence recovery of cytoplasmic TdTomato signal in photobleached spines. Left: tdTomato signal of spines transfected with SEP-GluA1 + tdTomato in the absence (dark blue; n=8) and the …
Figure 5 with 3 supplements
PKA and Epac activation are not sufficient to activate GluA2/3-plasticity.
(A) Example traces, mEPSC recordings of WT neurons with (n=12) or without (n=13) PKA agonist N002 in the recording pipette. N002 increased mEPSC frequency (t-test, p=0.004) and mEPSC amplitude …
Figure 5—figure supplement 1
PKA-inhibitor KT5720 prevents the forskolin driven increase in mEPSC amplitude, but not frequency.
Untreated wild-type CA1 neurons (n=7), neurons incubated with forskolin (n=8), with PKA inhibitor KT5720 (n=10), preincubated with KT5720 prior to forskolin (n=10). KT5720 blocked the …
Figure 5—figure supplement 2
Cyclic AMP does not directly activate GluA2/3-receptor complexes.
Conductance (left; paired t-test, p=0.4) and open probability (right; paired t-test, p=0.5) of somatic inside-out recordings from GluA1-KO CA1 neurons with S-AMPA (100 μM) in the recording pipette …
Figure 5—figure supplement 3
Farnesyltransferase antagonist Salirasib, a non-selective Ras inhibitor, partly blocks cAMP-driven synaptic potentiation.
Example traces, mEPSC frequencies and mEPSC amplitudes of untreated CA1 neurons (n=19), neurons incubated with forskolin (n=14), with Salirasib (n=18), preincubated with salirasib prior to forskolin …
Figure 6 with 2 supplements
cAMP activates GluA2/3-plasticity through a PKA- and Ras-dependent signaling pathway.
(A–B) Miniature EPSC recordings with anti-Ras IgG or control IgG in recording pipette from CA1 neurons in brain slices acutely isolated from mature wild type and GluA1-KO mice. After ten minutes …
Figure 6—figure supplement 1
The infusion of anti-Ras IgG or control IgG did not affect basal mEPSC frequency or amplitude.
Recordings as shown in Figure 6 were made in acute slices from WT (IgG: n=7; RasIgG: n=9; IgG/PKI: n=9; RasIgG/PKI: n=7) or GluA1-KO mice (IgG: n=11; RasIgG: n=11; IgG/PKI: n=8; RasIgG/PKI: n=12) in …
Figure 6—figure supplement 2
Frequency distribution of mEPSC events.
Distribution of mEPSCs per 1 pA binned amplitude of recordings shown in Figure 6 (+PKI condition) of (A) wild type neurons perfused with ctrl-IgG (left) or RAS-IgG (right) during baseline (black) …
Figure 7
β–adrenergic activation induces GluA2/3–plasticity.
Brain slices were acutely isolated from mature WT and littermate GluA3-KO mice, or GluA1-KO mice. Example traces, average mEPSC frequencies and amplitudes from CA1 neurons incubated with no drugs, …
Figure 8
NE release triggers the activation of GluA2/3-plasticity.
(A) Example traces and average motion as a change in significant motion pixels (SMPs) (Kopec et al., 2007) of WT injected with saline (n=15) or E (n=15), GluA3-KO injected with saline (n=17) or E …
Author response image 1
mEPSC frequency and amplitude of GluA3-deficient neurons, infected with GFP-GluA3 (blue) or GFP-GluA2Q (yellow) in the absence (even) or presence (blocked bar) of forskolin.
Author response image 2
Incubation of wild-type CA1 neurons with isoproterenol and IBMX increased the mEPSC frequency, but not amplitude.
Additional files
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Source data 1
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- https://doi.org/10.7554/eLife.25462.021
