(a) A schematic picture depicting flagella and the MT network in the cell body (top panel). Black dots, basal bodies (BB). Thick lines, four stable rootlet microtubule bundles. Thin lines in the … see more
(a) Schematics depicting an open-ended perfusion chamber (left panels) and a diffusion chamber (right panels) for capturing the HA-induced rapid changes. (b) A 10 μl aliquot of cells resuspended in … see more
(a) Concentration-dependent HA effects on MTs in WT cells. WT cells resuspended in 5 mM HA exhibited a bird cage pattern with dynamic comets (top and middle panels). Shrinking MTs were visible in … see more
(a) Static EB1 signals remained at BBs after cells were resuspended in 10 mM pH3 HA for 5 min (left panel). After replacing HA with the HEPES buffer, EB1 signal at the BB area intensified within 1 … see more
(a) The frozen bird cage pattern of fine MTs remains after 5 min HA bath as shown in two representative cells (left panel). After wash, dynamics resumes within 60 s. Notably, comets (panel I and II, … see more
(a) MTs in cells were largely frozen after 5 min 10 mM pH3 HA bath and 3 min in the wash solution, such as 5 mM pH7.4 Na+/HEPES buffer or 5 mM NaCl solution (black arrowheads). In contrast, growing … see more
(a) Reduced abundance and delayed appearance of sporangia with newly divided daughter cells as a function of NaCl concentration in TAP media. Triplicate cell pellets from a typical log-phase culture … see more
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Identical treatments were highlighted with a same gray shade for easy comparison. HA, acetic acid; TAP, Tris-Acetate-Phosphate culture medium.