Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila
- University of Virginia, United States
Figures
Figure 1 with 1 supplement
NB subtypes respond differently to dietary amino acid withdrawal.
(A) Experimental protocol to assay NB proliferation during dietary amino acid withdrawal. Freshly hatched (FH) larvae were transferred to a complete diet (yellow). Animals fed for 24 hr to …
Figure 1—figure supplement 1
Dietary nutrients are required for NB proliferation but do not affect NB number.
(A) Box plot of the number of Dpn-positive NBs in individual brain hemispheres at the indicated time points. Numbers in parentheses indicate number of brain hemispheres analyzed. (B) Box plot of …
Figure 2 with 1 supplement
MB NBs proliferate in a PI3-kinase-independent manner during dietary amino acid withdrawal, but not non-MB NBs.
(A–J) Maximum intensity projections of single brain hemispheres, top panel colored overlay with single-channel greyscale image below. Genotypes listed above and molecular markers to the left. Scale …
Figure 2—figure supplement 1
Altered PI3-kinase activity or reduced Alk levels do not affect EdU incorporation in MB NBs or their progeny.
(A) Box plot of the number of EdU-positive MB NBs at 7 d AFW for the indicated genotypes. p-values are 0.17, 0.37, and 0.50, respectively (Student's t-test). Numbers in parentheses indicate number …
Figure 3
Eyeless is expressed in MB NBs.
(A,E,I,M) Maximum intensity projections of single brain hemispheres, single-channel greyscale image in top panel with colored overlay below. Genotypes and developmental stage listed above and …
Figure 4
Eyeless is required for nutrient-independent MB NB proliferation.
(A,C,E) Maximum intensity projections of the four MB NBs (indicated by arrows) from the indicated genotype and time points. Brackets indicate two of the four MB NBs shown at higher magnification in …
Additional files
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Supplementary file 1
Candidate Drosophila metabolic genes regulated by Ey.
To assemble the list of candidate genes, regions 1 kb upstream and 200 bp downstream of annotated Drosophila promoters were searched using a position weight matrix of the Ey-binding site (Punzo et al., 2002). This set was then compared against a pre-compiled background dataset comprised of distribution scores for promoter-binding sites across the genome, allowing a determination of the likelihood of a given site as a putative Ey regulatory element. Candidates were further refined to genes with a known function in metabolism using Gene Ontology terms.
- https://doi.org/10.7554/eLife.26343.014
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Transparent reporting form
- https://doi.org/10.7554/eLife.26343.015
