1. Neuroscience
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Microtubule-dependent ribosome localization in C. elegans neurons

  1. Kentaro Noma
  2. Alexandr Goncharov
  3. Mark H Ellisman
  4. Yishi Jin  Is a corresponding author
  1. University of California, San Diego, United States
Research Article
  • Cited 19
  • Views 4,665
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Cite this article as: eLife 2017;6:e26376 doi: 10.7554/eLife.26376

Abstract

Subcellular localization of ribosomes defines the capacity for local translation. Methods to visualize ribosomes in live multicellular organisms are desirable for mechanistic investigations of the cell biology of ribosomes. Here, we developed an approach using split GFP for tissue-specific visualization of ribosomes in live Caenorhabditis elegans. Ribosomes are detected as puncta in the axons and synaptic terminals of specific neuron types, correlating with ribosome distribution at the ultrastructural level. We found that axonal ribosomes change localization during neuronal development and after axonal injury. Using genetic screens, we showed that the microtubule cytoskeleton and the JIP3 protein UNC-16 exert distinct effects on localization of axonal and somatic ribosomes. Our data demonstrate the utility of tissue-specific visualization of ribosomes in vivo, and provide insight into the mechanisms of active regulation of ribosome localization in neurons.

Article and author information

Author details

  1. Kentaro Noma

    Division of Biological Sciences, Neurobiology Section, University of California, San Diego, San Diego, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Alexandr Goncharov

    Division of Biological Sciences, Neurobiology Section, University of California, San Diego, San Diego, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Mark H Ellisman

    National Center for Research in Biological Systems, University of California, San Diego, San Diego, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Yishi Jin

    Division of Biological Sciences, Neurobiology Section, University of California, San Diego, San Diego, United States
    For correspondence
    yijin@ucsd.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9371-9860

Funding

Howard Hughes Medical Institute

  • Yishi Jin

National Institutes of Health (R01 035546)

  • Yishi Jin

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Anna Akhmanova, Utrecht University, Netherlands

Publication history

  1. Received: February 28, 2017
  2. Accepted: August 1, 2017
  3. Accepted Manuscript published: August 2, 2017 (version 1)
  4. Version of Record published: August 16, 2017 (version 2)

Copyright

© 2017, Noma et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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