Subcellular localization of ribosomes defines the capacity for local translation. Methods to visualize ribosomes in live multicellular organisms are desirable for mechanistic investigations of the cell biology of ribosomes. Here, we developed an approach using split GFP for tissue-specific visualization of ribosomes in live Caenorhabditis elegans. Ribosomes are detected as puncta in the axons and synaptic terminals of specific neuron types, correlating with ribosome distribution at the ultrastructural level. We found that axonal ribosomes change localization during neuronal development and after axonal injury. Using genetic screens, we showed that the microtubule cytoskeleton and the JIP3 protein UNC-16 exert distinct effects on localization of axonal and somatic ribosomes. Our data demonstrate the utility of tissue-specific visualization of ribosomes in vivo, and provide insight into the mechanisms of active regulation of ribosome localization in neurons.
- Yishi Jin
- Yishi Jin
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Anna Akhmanova, Utrecht University, Netherlands
© 2017, Noma et al.
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