(A) Three day sleep profile of CantonS flies, showing baseline day, interaction day and rebound day. At the end of interaction day, flies were mock transferred into a clean, fresh tube. (B) Quantification of rebound at ZT0-3 for the experiment shown in A. (C–H) Inactivation of ppk23 cells during rebound does not rescue the sleep phenotype. Parental control genotypes (C–E) flies carrying either the ppk23 GAL4 insertion (C,D) or the UAS-shiTS insertion (E,F). Experimental flies carrying both (G,H). Red traces indicate the sleep pattern of flies subjected to MF interaction at non-activating temperatures (22°C); on rebound day, temperature was raised to 29°C. Grey lines indicate the sleep profile of mock control flies that underwent the same temperature treatment but did not experience social interaction. (D, F, H) Quantification of sleep rebound during ZT0-3 of rebound day for flies shown in C, E. and G, respectively.