(A) A ‘neeps’ plot showing the distribution of log2 SILAC ratios measured in each of the G1, S, G2, and M subpopulations in one representative replicate. The width of each ‘neep’ is proportional to the density, that is the number of proteins. Quartiles are marked by black lines, and interquartile ranges are indicated by shading. B, inset) A schematic showing the cell division cycle and the average position during the cell division cycle for each phase collected, where a newly divided cell is defined as t = 0, and cell division (cytokinesis) is defined as t = 1. B, graph) Regression analysis was performed to produce a best-fit line in the form of an exponential growth model (i.e., y = emx). (C) Ratios of proteins belonging to each of the indicated GO terms were averaged (mean) and visualised using a heatmap. (D) A plot of ratios of individual proteins associated with mitosis, chromatin, and the stress response versus cell cycle stage. (E) A comparison of the ratio of G2 and M vs. asynchronous measured in the elutriation vs. PRIMMUS datasets.