1. Human Biology and Medicine
  2. Neuroscience
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Localization of spontaneous bursting neuronal activity in the preterm human brain with simultaneous EEG-fMRI

  1. Tomoki Arichi
  2. Kimberley Whitehead
  3. Giovanni Barone
  4. Ronit Pressler
  5. Francesco Padormo
  6. A David Edwards  Is a corresponding author
  7. Lorenzo Fabrizi  Is a corresponding author
  1. King’s College London, United Kingdom
  2. Imperial College London, United Kingdom
  3. University College London, United Kingdom
  4. Catholic University of Sacred Heart, Italy
  5. UCL-Institute of Child Health, United Kingdom
  6. Icahn School of Medicine at Mount Sinai, United States
Short Report
Cite this article as: eLife 2017;6:e27814 doi: 10.7554/eLife.27814
3 figures and 4 additional files

Figures

Delta brushes occur with distinct topographies.

Segments of EEG recordings showing individual examples of delta brushes with the most common topographical distributions (occurred at least three times in a given subject). These rarely involved frontal and midline electrodes which are therefore omitted for illustration clarity. Right and left posterior-temporal delta brushes occurred in 10/10 and 9/10 subjects respectively, while other delta brushes were recorded in no more than two subjects. EEG traces and recording electrodes where delta brush activity was maximal are marked in red. Shaded areas represent the time of occurrence of each event.

https://doi.org/10.7554/eLife.27814.002
Figure 2 with 2 supplements
Localization of posterior-temporal delta brushes.

In a group of 10 preterm infants (35 + 0 weeks PMA, range 32 + 2 to 36 + 2 weeks), right posterior-temporal delta brush activity (blue) was significantly associated with BOLD clusters in the right temporal pole (z50, z55), right superior temporal lobe (x28), and the right insular cortex (z60, z62, z64, z66). Left posterior-temporal delta brush activity (red-yellow) was significantly associated with BOLD clusters in the left posterior insula (z64, z66) and left parietal operculum (z66, z68). Images show the results of a one-sample t-test (p<0.05) performed using permutation testing and corrected for family-wise error overlaid on an age-specific T2-weighted brain atlas (Serag et al., 2012).

https://doi.org/10.7554/eLife.27814.003
Figure 2—figure supplement 1
Example of subject level analysis.

Hemodynamic activity correlated to right posterior-temporal delta brushes in a single infant of 35 + 1 weeks PMA. (a) Right posterior-temporal delta brush occurrence as identified by EEG (dashed vertical lines) was convolved with an age-appropriate hemodynamic response function to generate a general linear model regressor (blue) to fit to the acquired BOLD time series (red) for fMRI analysis. (b) Right posterior-temporal delta brush occurrence was significantly associated with well localized clusters of BOLD activity (red-yellow) in the right temporal pole and superior temporal lobe (z49); the left parietal operculum (z55); the anterior cingulate and right fronto-parietal lobes (z65 and z69). Images show the thresholded z-statistical map with a corrected cluster significance of p<0.05 overlaid on the subject’s T2-weighted image.

https://doi.org/10.7554/eLife.27814.004
Figure 2—figure supplement 2
T2-weighted MR images from two infants in our study sample imaged at 32 + 2 weeks PMA (top row) and 35 + 2 weeks PMA (bottom row).

As described in the literature, the subplate can be clearly seen as an area of high signal intensity (long T2) on the temporal poles bilaterally (red arrows).

https://doi.org/10.7554/eLife.27814.005
Localization of delta brush events in a single preterm infant.

Example of the significant hemodynamic activity correlated to less frequent delta brushes in a single preterm subject at 35 + 6 weeks PMA. (a) The occurrence of bilateral posterior-temporal delta brushes was significantly associated with well localized clusters of BOLD activity (red-yellow) in the bilateral superior temporal lobe and insulae (z55); while (b) bilateral occipital delta brushes were associated with a cluster in the medial occipital region (z33). Images show the thresholded z-statistical map with a corrected cluster significance of p<0.05 overlaid on the subject’s T2-weighted image.

https://doi.org/10.7554/eLife.27814.006

Additional files

Supplementary file 1

Demographic information of the study sample.

https://doi.org/10.7554/eLife.27814.007
Supplementary file 2

Delta brush topographical distributions.

In bold are topographical distributions that occurred at least three times for a given subject and were therefore used in the fMRI first level (individual subject) analysis. Delta brushes were unilateral (right – R or left – L), midline (M) or bilateral (B) frontal (F), central (C), temporal (T), parietal (Pa), posterior-temporal (PT), occipital (O), posterior-temporal occipital (PTO) or involving the posterior quadrant (PQ).

https://doi.org/10.7554/eLife.27814.008
Supplementary file 3

First level fMRI analysis results.

Summary of the spatial location of significant clusters identified in the first level analysis of the EEG-fMRI data for each subject with respect to the explanatory variables included in the model (delta brush topography).

https://doi.org/10.7554/eLife.27814.009
Transparent reporting form
https://doi.org/10.7554/eLife.27814.010

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