Loss of foxo rescues stem cell aging in Drosophila germ line
- University of Washington, United States
Abstract
Aging stem cells lose the capacity to properly respond to injury and regenerate their residing tissues. Here, we utilized the ability of Drosophila melanogaster germline stem cells (GSCs) to survive exposure to low doses of ionizing radiation (IR) as a model of adult stem cell injury and identified a regeneration defect in aging GSCs: while aging GSCs survive exposure to IR, they fail to reenter the cell cycle and regenerate the germline in a timely manner. Mechanistically, we identify foxo and mTOR homologue, Tor as important regulators of GSC quiescence following exposure to ionizing radiation. foxo is required for entry in quiescence, while Tor is essential for cell cycle reentry. Importantly, we further show that the lack of regeneration in aging germ line stem cells after IR can be rescued by loss of foxo.
Article and author information
Author details
Hannele Ruohola-Baker
Funding
National Institute of General Medical Sciences (R01-GM084947)
- Filippo Artoni
- Rebecca Kreipke
- Ondina Palmeira
- Connor Dixon
- Zachary Goldberg
National Institute on Aging (Genetic Approaches to Aging Training Grant)
- Rebecca Kreipke
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Artoni et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Loss of foxo rescues stem cell aging in Drosophila germ line
eLife 6:e27842.
https://doi.org/10.7554/eLife.27842
