(a–d) PSC (red and green) in wild-type, Cora or NrxIV knockdown LGs, (d) quantification of PSC cell numbers. (e–i) Plasmatocyte differentiation (red, white in e’,f’) in LG of control (e,e’,g), Cora knockdown (f,f’), cora (h;cora4/CyO), Nrx (i;Nrx4304/TM6B) heterozygous mutant alleles. PSC specific rescue of Cora (j’) or NrxIV (k’) in cora or NrxIV mutants compared to their respective controls (j,k). (l–p) Crystal Cell differentiation (red; white in l’,m’) in LG of control (l,l’,n), Cora knockdown (m,m’), cora (o;cora4/CyO), Nrx (p;Nrx4304/TM6B) heterozygous mutant alleles. PSC specific rescue of Cora (q’) or NrxIV (r’) in cora or NrxIV mutants compared to their respective controls (q,r). Quantitation of plasmatocyte (s) and crystal cell (t) differentiation for LGs with the phenotype shown in panels e-p as well as for a collection of hypomorphic mutations in SJ (septate junction) components as compared to the w1118 control. (a–c,e,f,j–k’,l,m,q–r’) PSC labeled with GFP (green; collier-Gal4 >UAS GFP) and/or Antennapedia (Red:a-c; white a’-c'). Plasmatocytes labeled with P1 (Red:e,f,g-k’;white:e’,f’), crystal cells labeled with Hnt (Red:l,m,n-r’; white:l’,m’). ***=P < 0.001. Error bars = s.e.m. PSC- specific Cora or NrxIV knockdown and mutant genotypes were compared to respective parental or wild type controls for the statistical analysis with the t-test. Scale Bar: (a–c’,e–r’) 50 μm.